1. Creating a heterozygous male Hermaphrodite Parent genotype Male Parent genotype X Male gametes Female gametes Progeny genotype Which progeny are you continuing with? Why? Progeny phenotypes and ratios

2. Hermaphrodite Parent genotype Male Parent genotype X Male gametes Female gametes F1 Progeny genotype and ratios F1 Progeny phenotypes Linkage 1: Cross with Unlinked unc +/+ ; unc/unc

3. Linkage 2: Selfing of hermaphrodite from unlinked cross hermaphrodite Xself cross gametes Progeny genotype Progeny phenotypes and ratios

4. Hermaphrodite Parent genotype Male Parent genotype X Male gametes Female gametes Progeny genotype Progeny phenotypes and ratios Linkage 1: Crossing with Linked unc Which progeny are you continuing with? Why? + unc/ + unc

5. Linkage 2: Selfing of hermaphrodite from linked cross hermaphrodite Xself cross gametes Progeny genotype Progeny phenotypes and ratios

6. Mapping 1: Making the double mutant hermaphrodite Xself cross gametes Progeny genotype Progeny phenotypes and ratios What if the genotype of the parent was + unc/+ unc?

7. Mapping 2: Making heterozygotes for mapping Hermaphrodite Parent genotype Male Parent genotype X Male gametes Female gametes Progeny genotype Progeny phenotypes and ratios Why might you see Dpy Unc progeny?

8. Mapping 3: Test Cross Hermaphrodite Parent genotype Male Parent genotype X Male gametes Female gametes Progeny genotype Progeny phenotypes and ratios Parental Recombinant

9. Symbols to Know for Crosses a//a – a is the allele and // represents the chromatids – there are two for a diploid organism – sometimes written as / a b//a b – two different genes on the same chromosome a//a ; b//b – two different genes on different chromosomes X – symbolizes mating between two different individuals X - symbolizes a self cross – when the hermaphrodite worms fertilize their own eggs