1. 2014 Protocol Sheets/Estrus Synchronization Planner

2. Products Make sure to give the correct injection on the day specified in the protocol Within product category, all products are equally effective Use at label dose Follow BQA guidelines for all injections GnRH PG Cystorelin ®, Factrel ®, Fertagyl ®, OvaCyst ® Estrumate ®, In-Synch ®, Lutalyse ®, ProstaMate ®, estroPLAN ®

4. Heifers– Heat Detection 1 Shot PG MGA ® -PG 12 PG 05 Heat detect & AI 7-day CIDR ® -PG 7 13 CIDR ® 0 PG Heat detect & AI MGA PG 114 3339 Heat detect & AI … 19 d … treatment day

5. Heifers – Heat Detect & TAI Select Synch + CIDR ® & TAI Heat detect and AI day 7 to 10 and TAI all non- responders 72 - 84 hr after PG with GnRH at TAI. MGA ® -PG & TAI Heat detect and AI day 33 to 36 and TAI all non- responders 72 - 84 hr after PG with GnRH at TAI. 710 CIDR ® 0 GnRHPG Heat detect & AI MGA PG 114 3336 Heat detect & AI … 19 d … treatment day GnRH AI.. 72 - 84 hr.. GnRH AI.. 72 - 84 hr.. CIDR PG 0143033 … 16 d … treatment day GnRH AI.70 – 74 hr 14-day CIDR ® -PG Heat detect and AI day 30 to 33 and TAI all non- responders 72 hr after PG with GnRH at TAI. Heat detect & AI

6. Heifers

7. Heifers– Short-Term TAI Protocols 7-day CO-Synch + CIDR ® Perform TAI at 54 ± 2 hr after PG with GnRH at TAI. 79 CIDR ® 0 GnRHPG treatment day GnRH AI.. 54 ± 2 hr.. 5 PG GnRH 0 8 AI CIDR ® …..60 ± 4 hr ….. treatment day PG 5-day CO-Synch + CIDR ® Perform TAI at 60 ± 4 hr after CIDR removal with GnRH at TAI. Two injections of PG 8 ± 2 hr apart are required for this protocol. …8±2 hr…

8. Heifers– Long-Term TAI Protocols MGA ® -PG Perform TAI at 72 ± 2 hr after PG with GnRH at TAI. MGA PG 1143336 … 19 d … treatment day GnRH AI. 72 ± 2 hr. CIDR PG 0143033 … 16 d … treatment day GnRH AI. 66 ± 2 hr. 14-day CIDR ® -PG Perform TAI at 66 ± 2 hr after PG with GnRH at TAI.

10. Cows – Heat Detection Select Synch 713 PG GnRH 06 Heat detect & AI Select Synch + CIDR ® 7 13 CIDR ® 0 GnRHPG Heat detect & AI treatment day

11. 9 PG Heat detect & AI 12 PG 6-day CIDR ® GnRH 30 PG Heat detect & AI Heat detect and AI days 0 to 3. Administer CIDR to non-responders and heat detect and AI days 9 to 12. Protocol may be used in heifers. CIDR ® treatment day Cows – Heat Detection

12. Cows – Heat Detect & TAI Select Synch & TAI Select Synch + CIDR ® & TAI Heat detect and AI day 6 to 10 and TAI all non-responders 72 – 84 hr after PG with GnRH at TAI. Heat detect and AI day 7 to 10 and TAI all non-responders 72 - 84 hr after PG with GnRH at TAI. 710 PG GnRH 06 Heat detect & AI 710 CIDR ® 0 GnRHPG Heat detect & AI treatment day GnRH AI.. 72 - 84 hr.. GnRH AI.. 72 - 84 hr..

13. PG 6-day CIDR ® & TAI Heat detect and AI days 0 to 3. Administer CIDR to non-responders and heat detect and AI days 9 to 12. TAI non-responders 72 - 84 hr after CIDR removal with GnRH at AI Protocol may be used in heifers. 9 PG Heat detect & AI 12 GnRH 30 PG Heat detect & AI CIDR ® treatment day GnRH AI.. 72 - 84 hr.. Cows – Heat Detect & TAI

14. Cows – Fixed-Time AI (TAI) CO-Synch + CIDR ® Perform TAI at 60 to 66 hr after PG with GnRH at TAI. 710 CIDR ® 0 GnRHPG treatment day GnRH AI.. 60 to 66 hr.. First version on recommended protocol sheets

15. 7-day CO-Synch + CIDR ® - Cows 7 PG GnRH 0 10 GnRH AI CIDR ® Perform TAI at 60 - 66 hr after PG with GnRH at TAI...60 - 66 hr.. treatment day 5 PG GnRH 0 8 AI CIDR ® …..72 ± 2 hr ….. treatment day PG 5-day CO-Synch + CIDR ® Perform TAI at 72 ± 2 hr after 1 st PG with GnRH at TAI. Two injections of PG 8 ± 2 hr apart are required for this protocol. …8±2 hr…

16. 5 PG GnRH 0 8 AI CIDR ® …..72 ± 2 hr ….. treatment day PG PG 5-day CO-Synch + CIDR ® Perform TAI at 66 ± 2 hr after 1 st PG with GnRH at TAI. Two injections of PG 8 ± 2 hr apart are required for this protocol. …8±2 hr… PG for Bos Indicus cows only

17. Features Recommended systems for cows & heifers Select systems by type –Heat detect & AI systems –Heat detect & cleanup AI systems –Fixed-Timed AI Systems List of daily activities Generates Barn Calendar Cost per AI pregnancy Support materials http://iowabeefcenter.org/estrus_synch.html Now free download updated 2014 version

18. www.estrussync.com

19. For iphone and androids

20. Net gain or loss from fixed-timed AI Rodgers et al., 2012

21. AI Cowculator Inputs Bull Maintenance cost Bull purchase price Useful life of bull Salvage value Interest rate Number of natural service bulls Number of bulls for clean up after AI Number of cows Expected weaning wt. Price of weaned calf AI labor cost AI facilities & equipment Synchronization products cost Semen cost Technician cost