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Molecular Cell, Vol. 7, 1213, 2001 Cdk1 Triggers Association of RNA Polymerase to Cell Cycle Promoters Only after Recruitment of the Mediator by SBF M.

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Presentation on theme: "Molecular Cell, Vol. 7, 1213, 2001 Cdk1 Triggers Association of RNA Polymerase to Cell Cycle Promoters Only after Recruitment of the Mediator by SBF M."— Presentation transcript:

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2 Molecular Cell, Vol. 7, 1213, 2001 Cdk1 Triggers Association of RNA Polymerase to Cell Cycle Promoters Only after Recruitment of the Mediator by SBF M. P. Cosma, S. Panizza, and K. Nasmyth Research Institute of Molecular Pathology Vienna, Austria

3 A URS1 se pega el FT Mbf (SWI5-SWI6) A URS2 se pega el FT SBF (SWI4-SWI6)

4 SWI5 FT SWI2 (Swi/Snf) remodel. ATP ADA2 SAGA) acetil histonas SWI4 FT Northern blot

5 Destino de la célula hija

6 ChIP-chip

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12 The mediator coactivator complex: functional and physical roles in transcriptional regulation Brian A. Lewis and Danny Reinberg Journal of Cell Science 116, © 2003

13 Principales conformaciones del Mediador humano

14 Estructura del mediador de levadura y del complejo con RNA polimerasa II

15 Correspondencia entre la figura compacta y extendida del mediador

16 Cambios conformacionales en el Mediador humano

17 Posible mecanismo de acción del Mediador

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25 Cromatinización del DNA Br Et revelado con sonda radiactiva; contra la region promotora en un caso y contra la region distal del plasmido en el otro

26 Posicionamiento traslacional nucleosoma

27 Posicionamiento rotacional (1)

28 Promotor de MMTV (1)

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30 m C DNA DNMT m C bind prot HDACs Active chromatin silent chromatin Transc.fact coactivators HATs

31 Marcas activas Marcas represivas H3-K4 mono, di, tri H3-K9 H3-K36 H3-K27 H3-K79 H4-K20

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35 Nco I

36 In vivo post viral infection Las lisinas de las histonas H3 y H4 no se acetilan al azar sino que especificamente: K8 y K12 en H4 y K9 y K14 en H3

37 Cell, Vol. 108, 475–487, 2002, Review: Cooperation between Complexes that Regulate Chromatin Structure and Transcription Geeta J. Narlikar, Hua-Ying Fan,and Robert E. Kingston

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39 Modelo de como la metilacion del DNA puede estar ligada a la H4-K20me3

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44 CDK7-ciclina H TFIIH Ser 5 CDK8-ciclina C Mediador Ser 2 y Ser 5 CDK9-ciclina T P-TEFb (positive transcrip. Elong. Factor) Ser 2 CTD humano 52 (min 28) CTD levadura 25 (min 9) CTD Drosophila 42

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