1. Confocal Laser Microscopy at the MHH

2. Laser Microscopy Facility Zentrale Forschungseinrichtung Lasermikroskopie Location: Building I4, Level 01 Contact: Rudolf Bauerfeind Wolfgang Posselt konfokal@mh-hannover.de Tel.: -3914

3. Confocal Laser Microscopy at the MHH Please, visit the web page! http://www.mh-hannover.de/lasermicroscopy.html

4. Confocal Laser Microscopy at the MHH How does a confocal microscope work? focal plane confocal pinhole

5. confocalwide field

7. Confocal Laser Microscopy at the MHH What is the advantage of using a confocal laser scanning microscope? optical sectioning 3D-imageing x-z sectioning better resolution easy multi colour fluorescence advantages of laser scanning bleaching (FRAP, …) ROI scan (photoactivatable fluorescent proteins) Katinka Döhner, Virology, MHH

8. Confocal Laser Microscopy at the MHH Which fluorescent dyes can I use? Available laser lines [nm] 405 442 458 476 488 496 514 543 594 633 MP 710-920 The choice is only limited by the excitation wave length

9. Confocal Laser Microscopy at the MHH Live cell imaging Incubation chambers with control of temperature, humidity, and CO 2 for cells grown on glass In situ microscopy using water immersion long distance objectives Christian Hähnel, Cell Biology

10. Confocal Laser Microscopy at the MHH Microscopes and applications

11. The laser microscopy facility is a S2 laboratory A cell culture with 2 safety hoods and 2 incubators can be used to prepare your experiments. Computer with full versions of the microscope software and 3d-imaging software are available Confocal Laser Microscopy at the MHH Additional facilities

12. Confocal Laser Microscopy at the MHH How can I use the facility? 1.Send an e-mail: konfokal@mh-hannover. 2.We will discuss your project. 3.A training session will instruct you in microscope operation and laser safety. Bring your own samples! 4.You can use the microscope on your own. Reservation are made through WWW. The facility is accessible 24 hr. 5.User will be charged with a user fee: 3-5 Euros per hr for MHH scientists.