1. Cdc42 Interacting Protein 4 (CIP4), Involvement in Endocytosis and Membrane Protrusion Genti Gjyzeli Kelly Mitok Corey O’Reilly

2. CIP4 Structure FBAR HR1 SH3 HR1 FBAR: Shimada et al., 2007 SH3: Miyamoto et al., unpublished HR1: Kobashigawa et al., 2009

3. CIP4 is involved in Endocytosis FB FBAR domain FBAR and HR1 domains Shimada et al., 2007 Pitts, 2005

4. Membrane Interacting Residues in Endocytosis Extracellular Intracellular Shimada et al., 2007

5. CIP4 in Endocytosis Extracellular Intracellular FBAR: Shimada et al., 2007 SH3: Miyamoto et al., unpublished HR1: Kobashigawa et al., 2009

6. Overexpression of CIP4 MicrotubulesActin Microtubule Overexpression of CIP4 in neurons gives large protrusion Normal Cortical Neuron ActinCIP4 Pictures provided by Dent and Saengsawang

7. CIP4 Location in Growth Cone CIP4Actin Video provided by Dent and Saengsawang

8. CIP4 Location in Growth Cone CIP4Actin Video provided by Dent and Saengsawang

9. Residues that may be important in protrusion Extracellular Intracellular Shimada et al., 2007

10. CIP4 in Protrusion Extracellular Intracellular FBAR: Shimada et al., 2007 SH3: Miyamoto et al., unpublished HR1: Kobashigawa et al., 2009

11. Future Mutation Studies to Determine Important Residues in Protrusion Shimada et al., 2007

12. Future Studies to analyze Potential Phosphoregulation of CIP4 Roberts-Galbraith et al., 2010

13. Diseases Associated with CIP4 Huntington’s Disease Cancer Joshi, 2010

14. Biocore Instructional Materials Learning Goals: – Predict how domains of a protein can influence it’s overall function – Electrostatic interactions of protein with membrane – One protein  one function – Predict protein function from location in cell – Endocytosis Materials: – Jmol tutorial – Cellular landscape – Animation

15. Thanks Michelle Harris Erik Dent Witchuda Saengsawang Tim, Mark, and Margaret at CBM of MSOE The other UW-Madison team for their feedback