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Initial results from the screening of PROSOPE and Roscoff cultures Florence LE GALL, Héloïse FELMAN, Frédéric PARTENSKY, Nathalie SIMON, Dominique MARIE,

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Presentation on theme: "Initial results from the screening of PROSOPE and Roscoff cultures Florence LE GALL, Héloïse FELMAN, Frédéric PARTENSKY, Nathalie SIMON, Dominique MARIE,"— Presentation transcript:

1 Initial results from the screening of PROSOPE and Roscoff cultures Florence LE GALL, Héloïse FELMAN, Frédéric PARTENSKY, Nathalie SIMON, Dominique MARIE, Daniel VAULOT

2 The first steps of isolation Surface water of ASTAN 2 filtrations on 3µm Enumeration by flow cytometry Culture in liquid medium K/10, K/100, K/10+Si,F/20 Serial dilutions K/10 Culture on plate K medium (0,6%agar)

3 Isolation and purification of microalgae Flow cytometry sorting Serial dilutions 160 precultures (PROSOPE) 38 strains isolated 24 eukaryotes 14 Synechococcus

4 Synechococcus spectra wavelength PROSOPE PUB PEB wavelength PROSOPE 53-4 PEB PUB

5 HPLC analysis and presence of caracteristic pigments PROSOPE culture 3 PROSOPE culture 18 MgDVPPrasViolaNeo Diadchr Chl b Chl a Neo Viola Lut Chl b Chl a ß-car MgDVP : Mg-2,4-divinyl pheoporphyrin a5 Pras : Prasinoxanthin Diadchr : Diadinochrome Neo : Neoxanthin Viola : Violaxanthin Chl b : Chlorophyll b Chl a : Chlorophyll a Lut : Lutein ß-car : ß-carotene Prasinophyceae Chlorophyceae

6 PROSOPE culture 96 ASTAN culture R Chl c2 Chl c3 19HF Fuco Diadino Chl a Chl c3 Chl c2 Fuco Diadino Diato Chl a ß-car 19HF :19-Hexanoyloxyfucoxanthin Fuco : Fucoxanthin Diadino : Diadinoxanthin Chl c2 : Chlorophyll c2 Chl c3 : Chlorophyll c3 Chl a : Chlorophyll a Diato : Diatoxanthin ß-car : ß-carotene Prymnesiophyceae Bacillariophyceae

7 Morphological characterization Optical microscopy Transmission electronic microscopy

8 Scatter Chl fluorescence Ostreo. ocea Bolid.med Bolid.pacifi Ostreo. taur Micro pusi Iso. Galb. E. huxleyi Phaeocystis PRO_3 PRO_22 PRO_5 Peuk Astan PRO_36 PRO_63 Euk Astan (K) PRO_114-2 PRO_18-1 PRO_96 PRO_118-1 PRO_90 Euk Astan (F/2) PRO_140-3 PRO_1 PRO_64 PRO_32 PRO_54 Prymnesiophyceae Bolidophyceae Prasinophyceae Chlorophyceae Prymnesiophyceae Cultures from - Med Sea (PRO) - Roscoff Diatom

9 Summary Prymnesiophyceae : 11 strains Prasinophyceae : 3 strains Chlorophyceae : 1 strain Bacillariophyceae : 1 strain


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