1. Phytophthora ramorum A short course Presented by the California Oak Mortality Task Force Part 3 - Regulations and Management of Phytophthora ramorum Background Photo Credits: Karl Buermeyer, UC Cooperative Extension (forest scenes, lone oak) Stephen Eales, Plant Health and Seed Inspectorate, DEFRA, UK (mountain laurel) Jan Hedberg, Oregon Department of Agriculture (viburnums in nursery) Steve Tjosvold, UC Cooperative Extension (rhododendron)

2. Course Outline Part 2 - Part 2 - Symptom Recognition, Diagnosis, and Sampling Part 1 - Introduction to Phytophthora ramorum and Sudden Oak Death Part 3 - Regulations and Management of Phytophthora ramorum Regulations and Quarantines Regulations and Quarantines Minimizing Spread in Wildlands and Landscapes Minimizing Spread in Wildlands and Landscapes Preventing Disease Transfer between Nurseries Preventing Disease Transfer between Nurseries Preventing Infection from Surrounding Woodlands Preventing Infection from Surrounding Woodlands Preventing Favorable Disease Conditions in the Nursery Preventing Favorable Disease Conditions in the Nursery Preventative Chemical Treatments Preventative Chemical Treatments Review Questions Review Questions

3. Part 3 Regulations and Management of Phytophthora ramorum Regulations and quarantines have been established to minimize the artificial spread of Phytophthora ramorum. Best Management Practices (BMPs) have been developed to aid in disease prevention.

4. In California, County Agricultural Commissioners and the California Department of Food and Agriculture are responsible for enforcing quarantines, and for dealing with nursery detections as well as wildland infestations. The California Department of Forestry and Fire Protection enforces regulations to prevent disease spread during timber operations on private land in infested areas. The USDA Animal and Plant Health Inspection Service (APHIS) regulates interstate commerce, and works with other countries to develop international shipping agreements. Regulations and Quarantines As of April 2004, countries with regulations on the importation of Phytophthora ramorum host material include: Canada, the UK, South Korea, New Zealand, Australia, the Czech Republic, Mexico, Taiwan, the US, the European Union, and others.

5. Since regulations and quarantines vary from region to region and are subject to change, it is best to refer to the following resources for up-to-date regulatory information: California Department of Food and Agriculture: www.cdfa.ca.gov/phpps/pe/sod_survey/ California County Agricultural Commissioners: www.cdfa.ca.gov/exec/cl/countyagmap.htm Oregon Department of Agriculture: www.oda.state.or.us/plant/ppd/path/SOD/ Washington State Department of Agriculture: agr.wa.gov USDA APHIS: www.aphis.usda.gov/ppq/ispm/sod/ Canadian Food Inspection Agency: www.inspection.gc.ca/english/plaveg/protect/dir/d-01-01e.shtml Department for Environment, Food and Rural Affairs (UK): www.defra.gov.uk/planth/pramorum.htm Users in states other than California, Washington, and Oregon should contact their state or county agriculture departments.

6. Minimizing Spread in Wildlands and Landscapes Always know when you are working or recreating in a Phytophthora ramorum-infested area so that proper sanitary measures can be practiced and to assure compliance with state and federal regulations. When in an infested area, especially where conditions are wet, keep vehicles on paved or gravel surfaces if possible. Before leaving the infested area, check yourself, pet(s), vehicle, and equipment to ensure that all mud, soil, organic material, and plant debris (leaves, twigs, etc.) are removed. Lysol , 70% alcohol, or a 10% bleach solution can be used to kill any remaining spores. Keep a cleaning kit (bucket, scrub brush, cleaning solution, & towels) in your vehicle, and use separate tools and footwear in infested areas if possible. As described in Part 1 of this course, under “Biology,” Phytophthora ramorum survives and spreads best under moist, cool conditions. Wet conditions can cause soil and plant material to adhere to vehicles, equipment, and footwear. Whenever possible, it is always best to avoid visiting an infested area, especially during the rainy season.

7. Dead and dying bark hosts rarely infect other trees. If dead or dying diseased trees are felled for hazard reduction, it is best to leave all material on-site when possible. An excellent way to dispose of larger woody material is to use it as firewood on-site. Smaller material can be chipped and used as mulch on-site, or lopped and scattered close to the ground to reduce fire hazard. While commercial composting of infested material is effective at killing the pathogen, homeowner composting is not recommended. If infested material must be removed from the site, it should be carefully covered and taken to an approved disposal site within the regulated area, such as an approved landfill, composting facility, or designated collection site for diseased material. Janice Alexander, UC Cooperative Extension

8. Preventing Disease Transfer between Nurseries To minimize the risk of infested plant material being transported between nurseries, the following BMPs* should be implemented : Confirm that host stock is propagated from on- site materials, or is received from shipping nurseries under compliance agreements. * Nursery BMPs developed by the COMTF Nursery Subcommittee- Karen Suslow, Hines Horticulture, and Gail Raabe, San Mateo Co. Agricultural Commissioner, Co-Chairs

9. Off-load incoming shipments to an area that can be cleaned of leafy debris. Sweep debris from the loading pad and truck, and bag it for disposal. Maintain accurate shipping documentation including product, amount, date, and origin to facilitate trace-backs and trace-forwards. Have trained personnel inspect all incoming host plants, regardless of origin, for Phytophthora ramorum symptoms prior to incorporating them into the production facility. Segregate these plants from other host plants, and periodically inspect them for symptoms over the course of a growing season.

10. If a nursery is located in an area where Phytophthora ramorum is found naturally, there are steps that should be taken to minimize the chance of nursery plants becoming infected. Preventing Infection from Surrounding Woodlands Monitor host plants in the surrounding area for disease symptoms, especially in the spring and summer. If practical, remove host plants within 100 feet of the nursery perimeter. Install diversion berms to prevent soil and water movement from surrounding hillsides during storm events.

11. Place containers/pots on a soil barrier such as gravel, or on raised beds. Monitor irrigation water from sources other than well or municipal water sources to ensure that it is pathogen-free. Overhead irrigation greatly increases the chances of introduction and spread of Phytophthora ramorum, especially in generally infested areas. Use drip irrigation wherever possible. If overhead watering must be used, irrigate in the morning to allow for drying by nightfall.

12. Should inoculum or infected plants enter the nursery, it is important to avoid conditions that may foster the pathogen’s survival and spread. The following precautions are recommended to help safeguard nurseries from internal infestations. Preventing Favorable Disease Conditions in the Nursery Shading and overhead irrigation create cool, humid conditions that, while favorable for some nursery crops, are also favorable for the spread and survival of Phytophthora ramorum. Avoid these conditions where possible. Correct low spots or poor drainage that may accumulate water for extended periods. Check and maintain irrigation systems to avoid leaks and uneven watering.

13. Wounded leaves are the most susceptible to infection. Wherever possible, avoid moving or handling plants during times when environ- mental conditions favor the pathogen. Locate soil and cull piles as far as possible from plants, and cover with clear polyethylene sheets. Dispose of culled plants and plant material as soon as possible. Make sure that all nursery workers are aware of the potential for Phytophthora ramorum infestations, and are familiar with symptoms for early detection.

14. Preventative Chemical Treatments While there is no known cure for Phytophthora ramorum, there is currently a preventative phosphite fungicide treatment available for use in high-risk landscape settings. In California, risk is largely based on proximity to California bay laurels. When treating a tree, the material is injected into the cambium, or sprayed directly on to the lower trunk of bark hosts. Two treatments 6 months apart are required the first year, with yearly treatments thereafter. As the treatment is relatively expensive and time consuming, it is not practical for wildland situations. Doug Schmidt, UC Berkeley

15. There is currently no eradicative treatment for Phytophthora ramorum on foliar hosts. Research is ongoing to find treatments to prevent foliar hosts, particularly ornamentals, from becoming infected and spreading the disease. Karl Buermeyer, UC Cooperative Extension

16. Review Questions 1. What are the two broad categories of measures taken to minimize the spread of Phytophthora ramorum? 1. Regulations and Quarantines 2. Best Management Practices 2. Name 3 levels of commerce that require regulating, and are under different jurisdictions. 1. Within a state (Intrastate) 2. Between states (Interstate) 3. International 3. At what time of year, or under what conditions, are extra precautions necessary when working in Phytophthora ramorum-infested areas? Under moist conditions or during wet times of the year

17. 4. What should always be done before leaving an infested area ? Review Questions, continued Remove host plant material and soil from footwear, vehicles, and equipment 5. What is the best way to dispose of infested plant material? Burn as firewood on the property, or leave it on site 6. List 4 BMPs to prevent Phytophthora ramorum spread between nurseries. 1. Confirm stock source is not infested 2. Inspect incoming host material for symptoms 3. Clean and dispose of debris from shipping area 4. Keep records of all shipping transactions

18. 7. Name 3 sources of infection for nursery plants in Phytophthora ramorum-infested areas. Review Questions, continued 1. Infected host plants around the perimeter 2. Soil and water runoff from hillsides 3. Irrigation water from infested water sources 8. What conditions in a nursery may make its environment more susceptible to Phytophthora ramorum infestations? Shading with overhead irrigation 9. What should be true about a host tree before considering it for preventative phosphite treatment? It should be a high-value tree, at risk of becoming infected

19. For additional information on Phytophthora ramorum and Sudden Oak Death, please visit the California Oak Mortality Task Force website: www.suddenoakdeath.org