1. Innovative Solution to Control Biofilm in Drains
Zep Biofilm Drain Purge A + B

2. Pathogens Reside in Biofilm/Challenges
Biofilms are natural habitat for microorganisms
Microorganisms in biofilms express resistance (1000 times the dose of antimicrobials required)
Sessile vs. planktonic (floating) organisms:
Sessile microorganisms embedded in a gelatinous matrix, anchored to a surface by polymeric sugars (polysaccharides)
Control of planktonic organisms does not necessarily mean control of biofilm (false sense of security)
PLANKTONIC
SESSILE 2

3. Issues In The Control of Biofilm
Biofilms more likely to form in wet, inaccessible areas and on corroded surfaces
Polymer matrix strongly adheres to surfaces
Conventional disinfectants and cleaners have limited effectiveness against biofilm
Not sufficient to kill the surface organisms – must remove the reservoir
Manual scrubbing is required (time consuming and is not effective in difficult to reach areas)
Failure to remove means repopulation in 48 hours – normally hours to re-establish
High risk of false negative sampling 3

4. Biofilms in Drains
2004, FSIS/USDA audit of food processing facilities and found 27.8% of drains are positive for Listeria
Drains - consistent food source and wet environment ideal for biofilm formation
Prime source of cross-contamination when drain pathogens are aerosolized 4

5. Bacterial Biofilm Can Move

6. Competitive Drain Programs
Sanitizer rings:
Can be expensive
Treat the water, not the drain
Do not remove biofilms
Liquids:
Do not provide full coverage of drain trunk line surfaces (upper part of the trunk line)
Do not have contact time
Do not remove biofilm
Can upset WWTP (Waste Water Treatment Plant) 6

7. Biofilm in Drains/Drain Trunk Line
Nutrition + Water + Oxygen + Temperature = Microbial growth (Slime/Biofilm)
Drain
Floor
Biofilm
Sanitizer
Solution
Drain Trunk Line

8. About PerQuatTM Technology
Active ingredients:
Quaternary Ammonium chloride 3%
Hydrogen Peroxide 3%
A powerful combination chemistry developed by pairing the negatively charged Perhydroxide ion with a positively charged Quaternary Ammonium compound. 
The two molecules form an intimate ion pair resulting in “PerQuat”, a Hydrogen Peroxide and Quat hybrid with unique cleaning and antimicrobial efficacy. 
This powerful technology can be used in a variety of industrial and public health environments to remove biofilm from the surfaces. 8

9. PerQuatTM Technology Biofilm Drain Purge
Alkaline peroxygen chemistry
Phase transfer catalysis
Hydrolysis of biofilm polymer matrix
Multiple antimicrobial mechanisms
Physical mechanisms
Attacks adhesions that glue biofilm
Collapses and removes biofilm polymer structure

10. The 3 Step Method To Remove Biofilms
PerQuatTM Technology
Step 1
Attack and bombard the biofilm matrix.
Step 2
Penetrate the outer layer and dissolve into the matrix.
Key
Step 3
Destroy and eliminate the biofilm matrix by a unique combination of hydrolysis, oxidation, and solubilization. 10

11. Surface Before and After Biofilm Drain Purge Treatment
PerQuatTM Technology
Surface prior to Biofilm Drain Purge Treatment
Surface after Biofilm Drain Purge Treatment 11

12. Biofilm Treatment with Different Sanitizers
Other products do not effectively remove biofilm
Before Bleach
After Bleach
Before Quat
After Quat
Before Glutaraldehyde
After Glutaraldehyde
PerQuatTM completely removes biofilm
Before Biofilm Drain Purge
After Biofilm Drain Purge 12 12

13. Biofilm Drain Purge Program
Biofilm Drain Purge A Prod. # 2766
Biofilm Drain Purge B Prod. # 2772
PerQuatTM Technology
Only EPA-registered drain program with biofilm claims
Concentrated, easy to use 2-part liquid
Designed to remove and control biofilms and biofouling
Non-acid, non-volatile
Chlorine and phosphate free
Can be flushed into sanitary waste systems

14. Biofilm Drain Purge Application
6 oz. Biofilm Drain Purge A + 6 oz. Biofilm Drain Purge B + 1 gallon of water in a foaming device
Foam Application (20 – 30 sec/drain)
Use any existing foaming equipment or Zep’s portable foaming devices
Required foaming attachment
No rinsing is required 14

15. Required Foaming Attachments
Drain Foam Attachment 7” (Prod. # N16801)
Drain Foam Attachment 12” (Prod. # N16701)
Black Drain Foam Plunger (Prod. # N16901)
Drain Foam Assembly (Prod.# N16601)

16. Foaming Attachments

17. Recommended Foamers 15 gallon rolling foamer product # 796401
2 gallon pump sprayer product # M79401

18. Biofilm Drain Purge Treatment in Drains/Drain Trunk Line
Foam Attachment
Drain
Floor
Biofilm Drain Purge Foam (Full coverage)
Biofilm
Drain Trunk Line

19. Biofilm in Drains/Drain Trunk Line

20. Benefits of Using Biofilm Drain Purge
Destroys and controls microorganisms in the biofilm in the drains
Eliminates fruit flies in the drains
Allows for an easy and quick foam application into drains and down into trunk lines
Allows for disinfection past the trap and a full coverage of the entire trunk line at 360°
No scrubbing is required
20 – 30 seconds/drain
Can be used on walls, floors and ceilings to
remove biofilms 20

21. Biofilm Drain Purge Use Applications
Food and beverage processing plants
Meat (all stages)
Poultry
Dairy
Wine
Beer
Beverage (fruit, soft drinks)
Food service restaurants and supermarkets
Dairy farms
Egg washing, grading, breaking facilities
Hatcheries
Environmental surfaces (walls, floors, ceilings)
Cooling water systems

22. Biofilm Drain Purge Literature
Biofilm Drain Purge Brochure ZDL a
Biofilm Drain Purge DVD on Zep Intranet

23. Questions?