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Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 1 Physiology and Biophysics of water and solutes transport *Departamento de Fisiología.

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Presentation on theme: "Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 1 Physiology and Biophysics of water and solutes transport *Departamento de Fisiología."— Presentation transcript:

1 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 1 Physiology and Biophysics of water and solutes transport *Departamento de Fisiología y Biofísica (Facultad de Medicina, Universidad de Buenos Aires), Buenos Aires, Argentina. (www.fmed.uba.ar) *Instituto de Física de Líquidos y Sistemas Biológicos (Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de la Plata, Comisión de Investigaciones Científcias de la Provincia de Buenos Aires), La Plata, Argentina. (www.iflysib.unlp.edu.ar) *CienciaNet (www.ciencianet.com.ar) Osvaldo Chara ochara@fmed.uba.ar ochara@iflysib.unlp.edu.ar

2 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 2

3 3 Transport of water and solutes… Transport of non electrolytes: Fick's laws The cup of coffee of Quilmes coffee sugar

4 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 4 Transport of non electrolytes: Fick's laws sugar coffee Transport of water and solutes… The cup of coffee of Quilmes

5 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 5 Transport of non electrolytes: Fick's laws sugar coffee Transport of water and solutes… The cup of coffee of Quilmes

6 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 6 Transport of non electrolytes: Fick's laws sugar coffee Transport of water and solutes… The cup of coffee of Quilmes

7 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 7 Transport of non electrolytes: Fick's laws What did it happen? Transport of water and solutes…

8 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 8 Transport of non electrolytes: Fick's laws The phenomenological explanation Imaginary membrane of area A and length  x JsJs Transport of water and solutes…

9 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 9 Transport of non electrolytes: Fick's laws A very beautiful equation! Transport of water and solutes…

10 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 10 Transport of non electrolytes: Fick's laws Now let see another phenomenon: How is the solute movement across a biological membrane? Transport of water and solutes…

11 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 11 Transport of non electrolytes: Fick's laws xx A C1C1 C2C2 Water + solute Transport of water and solutes…

12 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 12 Transport of non electrolytes: Fick's laws Suppose that both, water and solute can move across the membrane we will focus on the solute transport (Be patient: we will go to study water transport in 17 min) Transport of water and solutes…

13 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 13 Transport of non electrolytes: Fick's laws Suppose that C 1 > C 2 Transport of water and solutes…

14 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 14 Transport of non electrolytes: Fick's laws xx C2C2 C1C1 A Transport of water and solutes…

15 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 15 Transport of non electrolytes: Fick's laws Now, we will measure the transport of solute from one side to another Transport of water and solutes…

16 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 16 Transport of non electrolytes: Fick's laws xx C2C2 C1C1 A Js12Js12 Js12Js12 Transport of water and solutes…

17 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 17 Transport of non electrolytes: Fick's laws (Remember, C 1 > C 2 ) Which is the highest? ( J s 1  2 or J s 1  2 ) Transport of water and solutes…

18 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 18 Transport of non electrolytes: Fick's laws xx C2C2 C1C1 A Js12Js12 Js12Js12 Yeah! Transport of water and solutes…

19 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 19 Transport of non electrolytes: Fick's laws But what is the relation among J s ’s? Transport of water and solutes…

20 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 20 Transport of non electrolytes: Fick's laws The beautiful expression: Transport of water and solutes…

21 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 21 The concept of permeability But we must to know the length  x of the membrane. Transport of water and solutes…

22 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 22 Third week: Bioenergetics The concept of permeability The researchers of physiology and biophysics measure J s /A and  C

23 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 23 The concept of permeability Then, to achieve an equality… Transport of water and solutes…

24 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 24 The concept of permeability Therefore… Transport of water and solutes…

25 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 25 Do you remember an electrical circuit? Transport of water and solutes…  R

26 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 26 xx A C1C1 C2C2 Water + solute Transport of water and solutes…

27 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 27 Suppose that water can move across the membrane we will focus on the water transport (great!) Suppose that solute can not move across the membrane Transport of water and solutes…

28 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 28 Suppose that C 1 > C 2 Transport of water and solutes…

29 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 29 xx C2C2 C1C1 A Transport of water and solutes…

30 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 30 A phenomenological law of osmosis Transport of water and solutes…

31 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 31 Apical membrane Basolateral membrane Paracelular pathTranscelular path Transport of water and solutes…

32 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 32 Difussion - Partition Finkelstein, 1987 Range of P osm < 10 -3 cm.s -1 Verkman, 2000 Lipidic bilayer Transport of water and solutes…

33 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 33 Water channels: Aquaporins BA H 2 N HOOC C C 2 6 15 4 3 lumen citoplasma C C 2 6 15 4 3 2 6 15 4 3 lumen citoplasma lumen citoplasma A C C 2 6 15 4 3 lumen citoplasma A K A N P P A N C C 2 6 15 4 3 2 6 15 4 3 lumen citoplasma lumen citoplasma B K A N P P A N H 2 N HOOC H 2 N K A N P P A N Transport of water and solutes…

34 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 34 Water channels: Aquaporins Transport of water and solutes…

35 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 35 Range of P osm : (10 -3 - 10 -1) cm.s -1 Verkman, 2000 Permeability by unit channel Finkelstein, 1987 Water channels: Aquaporins Transport of water and solutes…

36 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 36 Water channels: Aquaporins AQPs! Transport of water and solutes…

37 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 37 Transport of water and solutes…

38 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 38 Na + -Glutamate (EAAT1) Na + /glucose (SGLT) Na + -K + -2Cl - (NKCC) Cotransports Transport of water and solutes…

39 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 39 versus ¿Cotransporter water-solute? Molecular water pump? Zeuthen 2002; Hamann y col, 2005 ¿Local osmosis? Duquette y col, 2001 Range of P osm  10 -3 cm.s -1 Hamann y col, 2005 Cotransporters Transport of water and solutes…

40 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 40 Transport of electrolytes: Nernst equation electrolytes Transport of water and solutes…

41 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 41 Third week: Bioenergetics electrolytes zoom Transport of electrolytes: Nernst equation

42 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 42 one molecule of electrolyte zoom Transport of electrolytes: Nernst equation Transport of water and solutes…

43 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 43 Let see… frictional force electrical force Transport of electrolytes: Nernst equation Transport of water and solutes…

44 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 44 The Flux Then Homogeneous concentration! Transport of electrolytes: Nernst equation Transport of water and solutes…

45 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 45 The Flux Thanks to Einstein we know that Then Transport of electrolytes: Nernst equation This is the Nernst-Planck equation Transport of water and solutes…

46 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 46 In equilibrium Then Transport of electrolytes: Nernst equation Transport of water and solutes…

47 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 47 Then Transport of electrolytes: Nernst equation But… The Nernst equation! Transport of water and solutes…

48 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 48 Third week: Bioenergetics Transport of electrolytes: Nernst equation Ups! The Nernst equation in a physiology book looks different!

49 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 49 Transport of electrolytes: Goldman equation Assuming … This is again the Nernst-Planck equation And … Transport of water and solutes…

50 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 50 Transport of electrolytes: Goldman equation Then … Integrating … Transport of water and solutes…

51 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 51 Transport of electrolytes: Goldman equation But remember … Therefore … Transport of water and solutes…

52 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara 52 Osvaldo Chara ochara@fmed.uba.ar ochara@iflysib.unlp.edu.ar *Departamento de Fisiología y Biofísica (Facultad de Medicina, Universidad de Buenos Aires), Buenos Aires, Argentina. (www.fmed.uba.ar) *Instituto de Física de Líquidos y Sistemas Biológicos (Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de la Plata, Comisión de Investigaciones Científcias de la Provincia de Buenos Aires), La Plata, Argentina. (www.iflysib.unlp.edu.ar) *CienciaNet (www.ciencianet.com.ar) Thank you! Transport of water and solutes…


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