Presentation is loading. Please wait.

Presentation is loading. Please wait.

Physiology and Biophysics of water and solutes transport

Published byCecilia Holland Modified over 9 years ago

Similar presentations

Presentation on theme: "Physiology and Biophysics of water and solutes transport"— Presentation transcript:

1 Physiology and Biophysics of water and solutes transport
Osvaldo Chara *Departamento de Fisiología y Biofísica (Facultad de Medicina, Universidad de Buenos Aires), Buenos Aires, Argentina. ( *Instituto de Física de Líquidos y Sistemas Biológicos (Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de la Plata, Comisión de Investigaciones Científcias de la Provincia de Buenos Aires), La Plata, Argentina. ( *CienciaNet ( Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

2 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

3 Transport of water and solutes…
Transport of non electrolytes: Fick's laws The cup of coffee of Quilmes coffee sugar Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

4 Transport of water and solutes…
Transport of non electrolytes: Fick's laws The cup of coffee of Quilmes coffee sugar Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

5 Transport of water and solutes…
Transport of non electrolytes: Fick's laws The cup of coffee of Quilmes coffee sugar Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

6 Transport of water and solutes…
Transport of non electrolytes: Fick's laws The cup of coffee of Quilmes coffee sugar Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

7 Transport of water and solutes…
Transport of non electrolytes: Fick's laws What did it happen? Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

8 Transport of water and solutes…
Transport of non electrolytes: Fick's laws The phenomenological explanation Imaginary membrane of area A and length x Js Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

9 Transport of water and solutes…
Transport of non electrolytes: Fick's laws A very beautiful equation! Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

10 Transport of water and solutes…
Transport of non electrolytes: Fick's laws Now let see another phenomenon: How is the solute movement across a biological membrane? Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

11 Transport of water and solutes…
Transport of non electrolytes: Fick's laws Water + solute Water + solute A C1 C2 x Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

12 Transport of water and solutes…
Transport of non electrolytes: Fick's laws Suppose that both, water and solute can move across the membrane we will focus on the solute transport (Be patient: we will go to study water transport in 17 min) Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

13 Transport of water and solutes…
Transport of non electrolytes: Fick's laws Suppose that C1 > C2 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

14 Transport of water and solutes…
Transport of non electrolytes: Fick's laws A C1 C2 x Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

15 Transport of water and solutes…
Transport of non electrolytes: Fick's laws Now, we will measure the transport of solute from one side to another Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

16 Transport of water and solutes…
Transport of non electrolytes: Fick's laws A C1 C2 Js12 Js12 x Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

17 Transport of water and solutes…
Transport of non electrolytes: Fick's laws Which is the highest? (Js12 or Js12) (Remember, C1 > C2) Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

18 Transport of water and solutes…
Transport of non electrolytes: Fick's laws A C1 C2 Js12 Yeah! Js12 x Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

19 Transport of water and solutes…
Transport of non electrolytes: Fick's laws But what is the relation among Js’s? Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

20 Transport of water and solutes…
Transport of non electrolytes: Fick's laws The beautiful expression: Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

21 Transport of water and solutes…
The concept of permeability But we must to know the length x of the membrane. Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

22 Third week: Bioenergetics
The concept of permeability The researchers of physiology and biophysics measure Js/A and C Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

23 Transport of water and solutes…
The concept of permeability Then, to achieve an equality… Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

24 Transport of water and solutes…
The concept of permeability Therefore… Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

25  Transport of water and solutes…
Do you remember an electrical circuit? R Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

26 Transport of water and solutes…
Water + solute Water + solute A C1 C2 x Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

27 Transport of water and solutes…
Suppose that water can move across the membrane Suppose that solute can not move across the membrane we will focus on the water transport (great!) Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

28 Transport of water and solutes…
Suppose that C1 > C2 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

29 Transport of water and solutes…
C1 C2 x Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

30 Transport of water and solutes…
A phenomenological law of osmosis Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

31 Transport of water and solutes…
Apical membrane Basolateral membrane Paracelular path Transcelular path Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

32 Transport of water and solutes…
Lipidic bilayer Difussion - Partition Finkelstein, 1987 Range of Posm < 10-3 cm.s-1 Verkman, 2000 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

33 Transport of water and solutes…
B A Water channels: Aquaporins H 2 N HOOC C 6 1 5 4 3 lumen citoplasma A K P B Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

34 Transport of water and solutes…
Water channels: Aquaporins Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

35 Transport of water and solutes…
Permeability by unit channelFinkelstein, 1987 Water channels: Aquaporins Range of Posm: ( ) cm.s-1 Verkman, 2000 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

36 Transport of water and solutes…
Water channels: Aquaporins AQPs! Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

37 Transport of water and solutes…
Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

38 Transport of water and solutes…
Cotransports Na+-Glutamate (EAAT1) Na+/glucose (SGLT) Na+-K+-2Cl- (NKCC) Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

39 Transport of water and solutes…
¿Cotransporter water-solute? Molecular water pump? Zeuthen 2002; Hamann y col, 2005 Cotransporters versus ¿Local osmosis? Duquette y col, 2001 Range of Posm  10-3 cm.s-1 Hamann y col, 2005 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

40 Transport of water and solutes…
Transport of electrolytes: Nernst equation electrolytes Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

41 Third week: Bioenergetics
Transport of electrolytes: Nernst equation electrolytes zoom Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

42 Transport of water and solutes…
Transport of electrolytes: Nernst equation one molecule of electrolyte zoom Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

43 Transport of water and solutes…
Transport of electrolytes: Nernst equation frictional force Let see… electrical force Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

44 Transport of water and solutes…
Transport of electrolytes: Nernst equation The Flux Then Homogeneous concentration! Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

45 Transport of water and solutes…
Transport of electrolytes: Nernst equation This is the Nernst-Planck equation The Flux Thanks to Einstein we know that Then Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

46 Transport of water and solutes…
Transport of electrolytes: Nernst equation In equilibrium Then Then Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

47 Transport of water and solutes…
Transport of electrolytes: Nernst equation Then But… The Nernst equation! Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

48 Third week: Bioenergetics
Transport of electrolytes: Nernst equation Ups! The Nernst equation in a physiology book looks different! Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

49 Transport of water and solutes…
Transport of electrolytes: Goldman equation This is again the Nernst-Planck equation Assuming … And … Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

50 Transport of water and solutes…
Transport of electrolytes: Goldman equation Then … Integrating … Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

51 Transport of water and solutes…
Transport of electrolytes: Goldman equation But remember … Therefore … Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

52 Transport of water and solutes…
Thank you! Osvaldo Chara *Departamento de Fisiología y Biofísica (Facultad de Medicina, Universidad de Buenos Aires), Buenos Aires, Argentina. ( *Instituto de Física de Líquidos y Sistemas Biológicos (Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de la Plata, Comisión de Investigaciones Científcias de la Provincia de Buenos Aires), La Plata, Argentina. ( *CienciaNet ( Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

Download ppt "Physiology and Biophysics of water and solutes transport"

Similar presentations

Membrane at Rest Selectively Permeable Channels Ion Concentration Differences Nernst Relationship Ion Pumps.

Membrane at Rest Selectively Permeable Channels Ion Concentration Differences Nernst Relationship Ion Pumps.
Chp 4 Transport of Solutes and Water. Review 1- The intracellular and extracellular fluids are similar in osmotic concentration but very different in.

Chp 4 Transport of Solutes and Water. Review 1- The intracellular and extracellular fluids are similar in osmotic concentration but very different in.
7-3: Cell Boundaries Biology 1.

7-3: Cell Boundaries Biology 1.
PHYSIOLOGY 1 Lecture 11 Membrane Potentials. n Objectives: Student should know –1. The basic principals of electricity –2. Membrane channels –3. Electrical-chemical.

PHYSIOLOGY 1 Lecture 11 Membrane Potentials. n Objectives: Student should know –1. The basic principals of electricity –2. Membrane channels –3. Electrical-chemical.
Cell Membrane Transport

Cell Membrane Transport
Movement Through the Membrane Cell Membrane. Cell Membrane… One of the main functions of the cell membrane is to regulate what enters and leaves the cell.

Movement Through the Membrane Cell Membrane. Cell Membrane… One of the main functions of the cell membrane is to regulate what enters and leaves the cell.
Lecture 2 27.11.2012 Diffusion, Osmosis and Osmotic Pressure.

Lecture Diffusion, Osmosis and Osmotic Pressure.
How do Materials move in & out of the Cell? They move across the cell membrane by: Passive Transport (no energy required) or Active Transport (energy required)

How do Materials move in & out of the Cell? They move across the cell membrane by: Passive Transport (no energy required) or Active Transport (energy required)
Cell Physiology Part 3 – Membrane Transport. 9-08-09 Agenda Review Membrane Potentials Membrane Transport –Passive –Active Summary of Membrane Function.

Cell Physiology Part 3 – Membrane Transport Agenda Review Membrane Potentials Membrane Transport –Passive –Active Summary of Membrane Function.
Passive Transport Section 4.1.

Passive Transport Section 4.1.
7:3 Movement Through the Membrane. Cell Membrane Every cell has a cell membrane Regulates what enters and leaves the cell. Provides protection & support.

7:3 Movement Through the Membrane. Cell Membrane Every cell has a cell membrane Regulates what enters and leaves the cell. Provides protection & support.
Cell Membrane.

Cell Membrane.
Announcements:. Last lecture 1.Organization of the nervous system 2.Introduction to the neuron Today – electrical potential 1.Generating membrane potential.

Announcements:. Last lecture 1.Organization of the nervous system 2.Introduction to the neuron Today – electrical potential 1.Generating membrane potential.
Chapter 4 Movement of Molecules Across Cell Diffusion Mediated transport system - faciliated transport - active transoprt Osmosis Endocytosis and exosctosis.

Chapter 4 Movement of Molecules Across Cell Diffusion Mediated transport system - faciliated transport - active transoprt Osmosis Endocytosis and exosctosis.
Cellular Transport Notes. Cell Membrane Outside of cell Inside of cell (cytoplasm) Cell membrane Proteins Protein channel Lipid bilayer Carbohydrate chains.

Cellular Transport Notes. Cell Membrane Outside of cell Inside of cell (cytoplasm) Cell membrane Proteins Protein channel Lipid bilayer Carbohydrate chains.
Micelles as Drug Carriers for Controlled Release

Micelles as Drug Carriers for Controlled Release
Transport of Molecules Across the Membrane

Transport of Molecules Across the Membrane
Transport: Passive and Active. Structure of Cell membranes Fluid not rigid Selectively permeable Made of a phospholipid bilayer Embedded with proteins.

Transport: Passive and Active. Structure of Cell membranes Fluid not rigid Selectively permeable Made of a phospholipid bilayer Embedded with proteins.
Cellular Transport. I. General A. Definition = molecules moving across the cell membrane B. Cell Membrane is selectively permeable (lets some things in,

Cellular Transport. I. General A. Definition = molecules moving across the cell membrane B. Cell Membrane is selectively permeable (lets some things in,
Membrane Transport and the Membrane Potential In Lecture Today: Cell membrane - Mechanisms of transport across the cell membrane: –Diffusion, and rate.

Membrane Transport and the Membrane Potential In Lecture Today: Cell membrane - Mechanisms of transport across the cell membrane: –Diffusion, and rate.

Similar presentations

Ads by Google