Presentation is loading. Please wait.

Presentation is loading. Please wait.

Physiology and Biophysics of water and solutes transport

Similar presentations


Presentation on theme: "Physiology and Biophysics of water and solutes transport"— Presentation transcript:

1 Physiology and Biophysics of water and solutes transport
Osvaldo Chara *Departamento de Fisiología y Biofísica (Facultad de Medicina, Universidad de Buenos Aires), Buenos Aires, Argentina. (www.fmed.uba.ar) *Instituto de Física de Líquidos y Sistemas Biológicos (Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de la Plata, Comisión de Investigaciones Científcias de la Provincia de Buenos Aires), La Plata, Argentina. (www.iflysib.unlp.edu.ar) *CienciaNet (www.ciencianet.com.ar) Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

2 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

3 Transport of water and solutes…
Transport of non electrolytes: Fick's laws The cup of coffee of Quilmes coffee sugar Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

4 Transport of water and solutes…
Transport of non electrolytes: Fick's laws The cup of coffee of Quilmes coffee sugar Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

5 Transport of water and solutes…
Transport of non electrolytes: Fick's laws The cup of coffee of Quilmes coffee sugar Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

6 Transport of water and solutes…
Transport of non electrolytes: Fick's laws The cup of coffee of Quilmes coffee sugar Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

7 Transport of water and solutes…
Transport of non electrolytes: Fick's laws What did it happen? Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

8 Transport of water and solutes…
Transport of non electrolytes: Fick's laws The phenomenological explanation Imaginary membrane of area A and length x Js Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

9 Transport of water and solutes…
Transport of non electrolytes: Fick's laws A very beautiful equation! Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

10 Transport of water and solutes…
Transport of non electrolytes: Fick's laws Now let see another phenomenon: How is the solute movement across a biological membrane? Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

11 Transport of water and solutes…
Transport of non electrolytes: Fick's laws Water + solute Water + solute A C1 C2 x Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

12 Transport of water and solutes…
Transport of non electrolytes: Fick's laws Suppose that both, water and solute can move across the membrane we will focus on the solute transport (Be patient: we will go to study water transport in 17 min) Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

13 Transport of water and solutes…
Transport of non electrolytes: Fick's laws Suppose that C1 > C2 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

14 Transport of water and solutes…
Transport of non electrolytes: Fick's laws A C1 C2 x Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

15 Transport of water and solutes…
Transport of non electrolytes: Fick's laws Now, we will measure the transport of solute from one side to another Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

16 Transport of water and solutes…
Transport of non electrolytes: Fick's laws A C1 C2 Js12 Js12 x Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

17 Transport of water and solutes…
Transport of non electrolytes: Fick's laws Which is the highest? (Js12 or Js12) (Remember, C1 > C2) Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

18 Transport of water and solutes…
Transport of non electrolytes: Fick's laws A C1 C2 Js12 Yeah! Js12 x Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

19 Transport of water and solutes…
Transport of non electrolytes: Fick's laws But what is the relation among Js’s? Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

20 Transport of water and solutes…
Transport of non electrolytes: Fick's laws The beautiful expression: Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

21 Transport of water and solutes…
The concept of permeability But we must to know the length x of the membrane. Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

22 Third week: Bioenergetics
The concept of permeability The researchers of physiology and biophysics measure Js/A and C Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

23 Transport of water and solutes…
The concept of permeability Then, to achieve an equality… Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

24 Transport of water and solutes…
The concept of permeability Therefore… Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

25  Transport of water and solutes…
Do you remember an electrical circuit? R Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

26 Transport of water and solutes…
Water + solute Water + solute A C1 C2 x Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

27 Transport of water and solutes…
Suppose that water can move across the membrane Suppose that solute can not move across the membrane we will focus on the water transport (great!) Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

28 Transport of water and solutes…
Suppose that C1 > C2 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

29 Transport of water and solutes…
C1 C2 x Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

30 Transport of water and solutes…
A phenomenological law of osmosis Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

31 Transport of water and solutes…
Apical membrane Basolateral membrane Paracelular path Transcelular path Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

32 Transport of water and solutes…
Lipidic bilayer Difussion - Partition Finkelstein, 1987 Range of Posm < 10-3 cm.s-1 Verkman, 2000 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

33 Transport of water and solutes…
B A Water channels: Aquaporins H 2 N HOOC C 6 1 5 4 3 lumen citoplasma A K P B Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

34 Transport of water and solutes…
Water channels: Aquaporins Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

35 Transport of water and solutes…
Permeability by unit channelFinkelstein, 1987 Water channels: Aquaporins Range of Posm: ( ) cm.s-1 Verkman, 2000 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

36 Transport of water and solutes…
Water channels: Aquaporins AQPs! Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

37 Transport of water and solutes…
Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

38 Transport of water and solutes…
Cotransports Na+-Glutamate (EAAT1) Na+/glucose (SGLT) Na+-K+-2Cl- (NKCC) Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

39 Transport of water and solutes…
¿Cotransporter water-solute? Molecular water pump? Zeuthen 2002; Hamann y col, 2005 Cotransporters versus ¿Local osmosis? Duquette y col, 2001 Range of Posm  10-3 cm.s-1 Hamann y col, 2005 Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

40 Transport of water and solutes…
Transport of electrolytes: Nernst equation electrolytes Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

41 Third week: Bioenergetics
Transport of electrolytes: Nernst equation electrolytes zoom Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

42 Transport of water and solutes…
Transport of electrolytes: Nernst equation one molecule of electrolyte zoom Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

43 Transport of water and solutes…
Transport of electrolytes: Nernst equation frictional force Let see… electrical force Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

44 Transport of water and solutes…
Transport of electrolytes: Nernst equation The Flux Then Homogeneous concentration! Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

45 Transport of water and solutes…
Transport of electrolytes: Nernst equation This is the Nernst-Planck equation The Flux Thanks to Einstein we know that Then Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

46 Transport of water and solutes…
Transport of electrolytes: Nernst equation In equilibrium Then Then Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

47 Transport of water and solutes…
Transport of electrolytes: Nernst equation Then But… The Nernst equation! Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

48 Third week: Bioenergetics
Transport of electrolytes: Nernst equation Ups! The Nernst equation in a physiology book looks different! Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

49 Transport of water and solutes…
Transport of electrolytes: Goldman equation This is again the Nernst-Planck equation Assuming … And … Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

50 Transport of water and solutes…
Transport of electrolytes: Goldman equation Then … Integrating … Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

51 Transport of water and solutes…
Transport of electrolytes: Goldman equation But remember … Therefore … Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara

52 Transport of water and solutes…
Thank you! Osvaldo Chara *Departamento de Fisiología y Biofísica (Facultad de Medicina, Universidad de Buenos Aires), Buenos Aires, Argentina. (www.fmed.uba.ar) *Instituto de Física de Líquidos y Sistemas Biológicos (Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de la Plata, Comisión de Investigaciones Científcias de la Provincia de Buenos Aires), La Plata, Argentina. (www.iflysib.unlp.edu.ar) *CienciaNet (www.ciencianet.com.ar) Fisiología y Biofísica del Transporte de agua y solutos - Osvaldo Chara


Download ppt "Physiology and Biophysics of water and solutes transport"

Similar presentations


Ads by Google