1. Aedes Mosquito Control Measures

2. Anti Larval Measure s Breeding source reduction is critical activity for Aedes control Treating the breeding source with chemical larvicide as an emergency measure and also for large unused container until it is destroyed or made Aedes proof

3. Application of Temephos Treat the breeding source with chemical larvicide namely Temephos 50% EC at the rate of 1 part per million. Note: The anti larval activity is for 1 week cycle which is based on the mosquito life cycle

4. LarvicideGenus Dilution / preparation of Stock Solution Dosage of Stock Solution Frequency TemephosAedes 2 ml in one litre of water 1 ml per 1 litre of stored water Weekly Temephos Formulation

5. Application of Temephos The stock solution should be applied @ 1 ml / 1 litre of water However, source reduction of mosquito breeding sites like tyre, coconut shells, grinding stones, plastic cups, broken pots etc is the best method of controlling Aedes mosquito breeding.

6. Application of Temephos All the water storage utensils sumps, cisterns etc should be closed tightly in order to prevent mosquito breeding. On an average 0.5ml of Temephos 50% EC is required to treat 250 litres of water sources in every house.

7. Anti Adult Measures Anti adult measures against Aedes is mainly indoor thermal fogging operation utilizing the mixture of 1 part of Pyrethrum 2% extract with 19 parts of diesel. This mixture (solution) is fogged indoors using specialized fog machines.

8. Anti Adult Measures The thermal fogging operation is to be done based on microplan Since the Aedes mosquitoes are active during day time, the thermal fogging operation is also conducted during the day time to achieve complete elimination of these vector mosquitoes

9. Adulticide Commercial Formulation Formulation for application Pyrethrum Extract2% 1:19 – 1 part of 2% Pyrethrum Extract in 19 parts of kerosene / diesel Adulticide Formulation

10. Guidelines on Thermal Fogging Thermal fogging is critical tool available for control Dengue / Chikungunya. The selection of places, time, type of machines, insecticide and formulation plays a pivotal role in the operation

11. Type of Application Type of Fogging Machine to be used Indoor Hand operated mini fog machine Portable shoulder held thermal fogging machine Outdoor Portable shoulder held thermal fogging machine Vehicle Mounted Fogging Machine

12. 1. Indoor fogging has to be done for Aedes mosquito control. 2. Indoor fogging means, fogging the insecticide using portable fogging machine or mini hand fog machine inside the house and in the peri domestic area. Indoor Fogging Guidelines

13. 3. 3. Residents should be educated before the fogging operation to keep the food covered, children and all inmates are kept outside the house. 4. All windows and doors to be closed for at least 15 minutes after the fogging operation to keep the fog inside. Indoor Fogging Guidelines

14. 5.For a single storey building fogging to be done through the front door, for multi storey building the fogging to be carried out from upper floor to the lower floor. 6.On an average 200 ml of Pyrethrum and 4 litre of diesel are mixed for fogging and 1 litre of petrol is required for running the machine to fog 100 houses or 500 population with portable fogging machine.

15. 7. On an average 125 ml of Pyrethrum, 2.5 litre diesel is mixed for fogging and 1 LPG cylinder is required for running the machine to fog 60 houses or 300 population with mini fogging machine. Indoor Fogging Guidelines

16. Outdoor Fogging 1.Outdoor Fogging Operation to be done always against the wind. 2. For vehicle mounted fog machine the vehicle should move at a speed of 6 to 8 kmph. 3. At least one round of the fogging should be carried out within each breeding cycle i.e. 8 to 10 days.

17. 4. On an average 60 litre of pyrethrum, 114 litre diesel are mixed for fogging and 20 litre petrol is required for running the machine fogging 1300 houses or 6500 population with Vehicle Mounted Fogging Machine. Outdoor Fogging

18. Outdoor Fogging Timings For Aedes control fogging has to be done during day time between 8 am to 5 pm preferably Morning – 8 am to 11 am Evening – 3 pm to 5 pm

19. Measures for Assessing the impact of Aedes Control Measures

20. HI = X 100 No. of Houses infested No. of Houses Inspected (>10% - High risk ; <1% - Low risk) House Index

21. Container index The percentage of water holding containers infested with Aedes larvae or pupae No. of positive containers No. of containers Inspected CI = X 100

22. No. of positive containers per 100 inspected houses No. of positive containers No. of Houses inspected BI = X 100 (> 50 % - High risk; < 5% - Low risk ) Breteau Index

23. Number of pupae per house Number of pupae Number of Houses inspected PI = X 100 Pupal Index

24. Tools to be available with every team Torch light Chloroscope Hammer Crow bar Laddle Pamphlets Temephos Fogging machines Bleaching powder