Presentation on theme: "INDIA /BRAZIL / SOUTH AFRICA TRI-LATERAL"— Presentation transcript:
1 INDIA /BRAZIL / SOUTH AFRICA TRI-LATERAL COLLABORATIVE PROGRAM ON SCIENCE AND TECHNOLOGYII Forum IBSA on Malaria, Teresina – PI, BR, March, 7th - 8th, 2006Hernando A del Portillo, PhD Departamento de ParasitologiaInstituto de Ciências Biomédicas Universidade de São Paulo
5 Giemsa-staining Malaria Diagnosis Brazil 1960-2005
6 External Evaluation of Giemsa-staining diagnosis in reference laboratories from the State of Pará Dra. Marinete PóvoaState of Pará more than cases/200511 participating laboratoriesFive different reference slides: P. falciparum, P. vivax, mixed infection, P. vivax + T. cruzi, negative control73% of the labs diagnosed correctly all slides, remaining 27% miss-diagnosed 1 slide.None of the labs identified T. cruzi.5 labs have adequate training; 4 labs require re-training and 2 labs require capacitance.
7 Multi-Center Assesstment of Immuno-chromatographic Methods Coordinated: Prof. Cor Jésus Fernandes Fontes
8 Giemsa Staining Total No of Patients : 539 Resuts given by Manaus, Porto Velho, Macapá e Cuiabá.Giemsa StainingFVNTotal87289OPTIMALV*10449333346Total91113335539*=Positive results of other humana malaria parasite species,here in considered positive forP. vivaxSensitivityP.falciparum= 95,6%P.vivax= 92,0Specificity= 99,5%= 100,0%Overall sensitivity for malaria diagnosis= 93,6%Overall specificity for malaria diagnosis= 99,4%
12 NESTED-PCREvaluation of negativation and detection of three human malaria parasite species: Marinete PóvoaBlood bank surveys Ricardo MachadoMF Ferreira-Da-CruzMixed Infections Graça AlecrimAsymptomatics LH Pereira da Silva
13 Nested-PCR and Malaria Diagnosis at FMT/AM Nested-PCR detected 14.5% of mixed infections as opossed to 1,8% detected by Giemsa staining
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