1. © Mibelle Biochemistry, Switzerland 2007 Trimoist 24h hydration and protection

2. © Mibelle Biochemistry, Switzerland 2007 Concept of the Moisture Balance Theory water lamellar lipids humectants Trimoist water NMF skin Brick and mortar model Hydrolipid film Protecting film

3. © Mibelle Biochemistry, Switzerland 2007 Structure of the Skin protecting film lamellar lipid barrier humectants

4. © Mibelle Biochemistry, Switzerland 2007 Structure of the Skin Aqueous space Lipids Stratum corneumLipid layerIntercellular space Ceramides Phospholipids Triglycerides Fatty acids Cholesterol Corneocytes

5. © Mibelle Biochemistry, Switzerland 2007 Protein-Glycation frying, baking, roasting Maillard-Reaction Non-enzymatic interaction of sugars and proteins at high temperatures or over a long time Brown color skin Protein glycation Collagen and elastin react with sugars -> AGEs (advanced glycation endproducts) Loss of elasticty, dull and wrinkled skin

6. © Mibelle Biochemistry, Switzerland 2007 Result of Skin Glycation Irreversible cross-links between adjacent protein molecules Cross-linking significantly contributes to the stiffening and loss of elasticity found in aged tissues

7. © Mibelle Biochemistry, Switzerland 2007 Collagen Carnosine Sugar Collagen fibre Cross-linked and stiffened collagen With Carnosine: Collagen fibers are protected and stay elastic Formation of AGE's

8. © Mibelle Biochemistry, Switzerland 2007 Glycation and Diabetes People with diabetes, who have high glucose levels, show accelerated aging. Results already in young people: stiffening connective tissue (collagen) hardened arteries clouded eyes loss of nerve function less efficient kidneys

9. © Mibelle Biochemistry, Switzerland 2007 Formation of AGEs + C OO (CHOH) 4 CH 2 OH NH 2 NH CH (CHOH) 4 CH 2 OH NH CH 2 (CHOH) 3 C O CH 2 OH glucose proteinSchiff base Amadori product N N + Lysine Lysine N N N + N H Arginine Lysine CHNHC O (CH 2 ) 4 NH CH 2 COOH advanced glycation end products CMLpentosidine MOLD

10. © Mibelle Biochemistry, Switzerland 2007 Anti-Glycation Carnosine naturally occurring a peptide-like compound with a free amino group reacts with the aldehyde or ketone group of reducing sugars Competitive inhibitor of AGE's

11. © Mibelle Biochemistry, Switzerland 2007 Carnosine Prevents Formation of MDA UV-A Lipidperoxidation Malondialdehyde (MDA) Protein carbonylation, AGE formation Carnosine

12. © Mibelle Biochemistry, Switzerland 2007 Active factors in Trimoist 1 Protecting film CM-GlucanProtecting film, improves skin elasticity, stimulates self protecting capacity of the skin 2 Lamellar lipid structur Stearyl lactate Cetyl alcohol Oleic acid triglyceride Phytosterols Vitamin EAcetate Form a basic lamellar lipid structure in water Enhance the barrier function of the lamellar lipid layer 3 Humectants Glycerin Lactate Bind high levels of water + Anti-Glycation CarnosineReacts with reducting sugars and thus inhibits formation of AGEs

13. © Mibelle Biochemistry, Switzerland 2007 Increase in Skin Hydration 0.00 4.00 8.00 12.00 16.00 20.00 after 3 daysafter 5 days Increase in skin hydration (%) S-054 5% Hygroplex5% Trimoist

14. © Mibelle Biochemistry, Switzerland 2007 Increase in Skin Firmness 0.00 5.00 10.00 15.00 20.00 25.00 30.00 35.00 after 3 daysafter 5 days Increase in skin firmness (%) 5% Hygroplex5% Trimoist S-054

15. © Mibelle Biochemistry, Switzerland 2007 Increase in Skin Smoothness 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 after 3 daysafter 5 days Increase in skin smoothness (%) S-054 5% Hygroplex5% Trimoist

16. © Mibelle Biochemistry, Switzerland 2007 Increase in Skin Hydration 0 2 4 6 8 10 12 14 16 18 7 days14 days Increase in skin hydration [%] Hydrogel + 1% Trimoist Hydrogel + 2% Trimoist Hydrogel + 5% Trimoist Hydrogel + 5% Hygroplex S-058

17. © Mibelle Biochemistry, Switzerland 2007 24 h hydration effect 20 25 30 35 40 45 50 55 60 65 024681224 hoursafterapplication moisture [a. u.] untreated emulsionwith2% Trimoist * * * * * * p<0.05versusuntreated 20 25 30 35 40 45 50 55 60 65 024681224 afterapplication moisture [a. u.] untreated emulsionwith2% Trimoist * * * * * * p<0.05versusuntreated 20 25 30 35 40 45 50 55 60 65 024681224 afterapplication moisture [a. u.] untreated emulsionwith2% Trimoist * * * * * * p<0.05versusuntreated 20 25 30 35 40 45 50 55 60 65 024681224 afterapplication moisture [a. u.] untreated emulsionwith2% Trimoist * * * * * * * p<0.05versusuntreated S-063

18. © Mibelle Biochemistry, Switzerland 2007 Benefits for R & D Staff 1. Trimoist is an active suitable for a great variety of cosmetic topics: - Moisturizing - Anti-Aging - Firmness - Protection - Anti-Glycation 2. Trimoist in an active containing a large number of up-to date ingredients: - lamellar lipids - NMF/Humectants - Protecting Polysaccharide - Phytosterols - Dipeptide (Carnosine)

19. © Mibelle Biochemistry, Switzerland 2007 Benefits for R & D Staff 3. Trimoist in an active documented with a large number of dermatological studies : - Skin Hydration - Skin Elasticity - Skin Smoothing - Film Protection - Anti-Glycation - Skin Regeneration - Antioxidant Status - Protection against oxidative stress

20. © Mibelle Biochemistry, Switzerland 2007 Résumé Trimoist- the 3-step moisturizing system supports all three elements of the skin's own moisture balance system: 1. Protecting film CM-Glucan enhances the skin's own defence system and builds a superior protecting film. 2. Lamellar Lipid barrier Skin-like Lamellar Lipids enhance the barrier function of the lipid layer in the epidermis to boost the moisture barrier. 3. Humectants Glycerin and Lactate mimic the Natural Moisturizing Factor (NMF) of the skin to provide an instant burst of hydration.  Carnosine prevents the cross-linking and stiffening of collagen by glycation - the technical term for the process that leads to a loss of skin-elasticity

21. © Mibelle Biochemistry, Switzerland 2007 Trimoist … keeps skin at its best: softer, smoother, even-toned, visibly less lined. Hydrate and protect your skin with the 3-Step Moisturizing System of Trimoist…

22. © Mibelle Biochemistry, Switzerland 2007 0 1 2 3 4 5 6 7 Squalene peroxides [units CL] nt 0%0,04%0,2% CM-Glucan in o/w emulsions nt UV-A 10 J/cm 2 CM-Glucan: Photo Protection

23. © Mibelle Biochemistry, Switzerland 2007 CM-Glucan: Film Protection 0% 0.1% 0.4% 0.04% 0% 0 20 40 60 80 Protection relative to untreated skin [%] hydrogelo/wemulsion CM-Glucan Skin humidity