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In vitro evaluation of biomaterials for bone tissue engineering Biomaterials Dept. Materials Science and Technology University of Crete, P.O. Box 2208.

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Presentation on theme: "In vitro evaluation of biomaterials for bone tissue engineering Biomaterials Dept. Materials Science and Technology University of Crete, P.O. Box 2208."— Presentation transcript:

1 In vitro evaluation of biomaterials for bone tissue engineering Biomaterials Dept. Materials Science and Technology University of Crete, P.O. Box 2208 GR- 71303 Heraklio, Crete, Greece mchatzin@materials.uoc.gr Maria Chatzinikolaidou

2 Research activities In vitro biocompatibility study of biomaterials, including cell adhesion, viability, proliferation and morphology of mammalian cell lines and stem cells Investigation of the osteoinductive potential of implantable biomaterials  bone tissue repair Development of immobilization techniques for proteins on biomaterials surfaces Adsorption and desorption studies of proteins on biomaterials surfaces

3 Cell adhesion shown by SEM Cell source: Bone marrow mesenchymal stem cells from donors’ iliac crest Biomaterial: organic-inorganic composite material, structured by two- photon polymerization After 3 days In collaboration with Prof. Maria Vamvakaki, Dr. Maria Farsari and Prof. Eleni Papadaki After 4 hours

4 Cell adhesion and proliferation shown by SEM Cell source: Bone marrow mesenchymal stem cells from donors’ iliac crest Biomaterial: organic-inorganic composite material, structured by two- photon polymerization After 6 days In collaboration with Prof. Maria Vamvakaki, Dr. Maria Farsari and Prof. Eleni Papadaki

5 Cell adhesion shown by SEM Cell source: Bone marrow mesenchymal stem cells from donors’ iliac crest Biomaterial: organic-inorganic composite material – meshes structured by two-photon polymerization After 4 hours In collaboration with Prof. Maria Vamvakaki, Dr. Maria Farsari and Prof. Eleni Papadaki

6 Cell formations during adhesion Extracellular factors and integrin-derived signaling trigger activate actin polymerization at the leading-edge membrane (as an early event) and the formation of focal adhesions (as a later event) Early event Biomaterials: The Intersection of Biology and Materials ScienceJohnna S. Temenoff, Antonios G. Mikos Late event

7 Cell morphology of MC3T3-E1 Visualization of vinculin and actin cytoskeleton by means of confocal microscopy Overlay of double staining with ΤRΙTC-conjugated falloifin and FITC-conjugated anti-vinculin magnification 20x Cell source: mouse pre-osteoblasts MC3T3-E1 Biomaterial: organic-inorganic composite material

8 Cell morphology Visualization of vinculin and actin cytoskeleton by means of confocal microscopy Overlay of double staining with ΤRΙTC-conjugated falloifin and FITC-conjugated anti-vinculin magnification 40x Cell source: mouse pre-osteoblasts MC3T3-E1 Biomaterial: organic-inorganic composite material

9 Acknowledgements Collaborations Prof. Maria Vamvakaki, UoC Dr. Maria Farsari, Prof. Costas Fotakis, IESL-FORTH Prof. Eleni Papadaki, UoC

10 A reservoir for progenitor cells http://anatquest.nlm.nih.gov

11 Organic-inorganic composite material Methacryloxypropyl trimethoxysilane (MAPTMS), (2-dimethylamino)ethyl methacrylate (DMAEMA) and methacrylic acid (MAA) as the polymerizable monomers Zirconium n-propoxide (Zr(OPr) 4, and the trimethoxysilane groups of MAPTMS serve as the inorganic network forming moieties

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