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CTRC Safety Training March 10, 2014

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Presentation on theme: "CTRC Safety Training March 10, 2014"— Presentation transcript:

1 CTRC Safety Training March 10, 2014

2 Purpose Provide Required Annual Safety Training for Personnel at the CTRC

3 Personnel and Responsibilities
CTRC Director- Dr Timothy Murphy CTRC Manager- Dr. Richard Karalus UB Biosafety Officer- David Pawlowski, Ph.D UB Radiation Safety Officer- Jeff Slawson Environmental Programs Manager- Brian Foti Chemical Hygiene Officer, Hazardous Materials Manager- Anthony Oswald Environmental Health and Safety Services Employer (PI) responsibilities Employee responsibilities PIs bear full responsibility for safety in their laboratories

4 Fire Safety

5 Fire Safety and Prevention
During a fire Turn off oxygen, gas, and electrical equipment in the affected area USE THE STAIRS – NEVER USE THE ELEVATORS Know primary and secondary evacuation routes

6 Fire Safety and Prevention
Bunsen Burners/open flame devices should be used only when necessary and should always be attended. Consider bacticinerators and micro burners as alternatives to Bunsen Burners. Biosafety Cabinets - open flames are NOT recommended. They can damage the HEPA filter and cause a fire.

7 Fire Safety and Prevention
Extension cords are not permitted for permanent applications Space heaters must be equipped with tip over shutoff devices Daisy Chained power strips are not permitted Maintain a minimum of 18" between boxes and ceiling

8 Safety Equipment Fire alarm pull stations Fire extinguishers
Each stairwell entrance CRC Fire extinguishers Emergency showers Emergency eye wash stations AEDs near SW corner of each floor First aid kits Know locations of safety equipment

9 Fire Safety Fire alarm pull stations are located by each stair well
Fire alarms will include both strobe and audio alarms CTRC alarms only alarm floor involved and the adjacent floors Only alarming floors are required to evacuate unless otherwise advised

10 Fire Extinguishers Fire Extinguishers: Located in most laboratories
Do not obstruct or conceal fire extinguishers Located in hallways throughout CTRC Know where your nearest fire extinguisher is

11 Fire Extinguishers P = Pull the pin
When using remember P.A.S.S. P = Pull the pin A = Aim at base of fire about 8-10ft away S = Squeeze the trigger S = Sweep side to side “How to Use” instructions can be found on the fire extinguisher label USE ONLY IF FIRE IS SMALL AND IF YOU HAVE HAD TRAINING ON ITS USE

12 Fire: Remember R.A.C.E. R = Rescue Rescue people in the A = Announce
immediate area A = Announce Announce the fire verbally Activate the alarm C = Confine Confine fire by Closing doors E = Evacuate Evacuate the floor, Extinguish if a small fire

13 Evacuation-Fifth Floor

14 Evacuation-Sixth Floor

15 Evacuation-Seventh Floor

16 Evacuation-Eighth Floor

17 Section #3 Ground Floor Evacuation

18 Sub-basement Level Evacuation

19 General Laboratory Safety

20 Biosafety levels BSL-1 BSL-2 BSL-3 BSL-4
Organisms that do not normally cause human infections BSL-2 Organisms that cause human infections of low morbidity/mortality Potential for aerosol transmission BSL-3 Organisms that cause human infections of high morbidity/mortality Aerosol transmission BSL-4 Organisms of extremely high morbidity/mortality for which there are no treatments Aerosol or unknown transmission

21 General Safety General laboratory rules Minimize aerosols Wash Hands
Eating, drinking, smoking or the use of other tobacco products or cosmetics is strictly prohibited The application or removal of contact lenses is forbidden Storage of these items in the laboratory is prohibited Mouth pipetting is strictly prohibited Open toed shoes are not permitted Minimize aerosols Wash Hands

22 Personal Protective Equipment
Should never serve as primary protection Appropriate for risk Minimum Disposable surgical gloves Nitrile (recommended) Latex Eye protection Splash UV/other radiation Lab Coat Respirators (may require fit testing and medical clearance- contact EH&S) Particulate (N95, PAPR, P-100) Chemical cartridge (activated charcoal, chlorine, etc) Hearing

23 PPE Rules to Remember Always check PPE for defects or tears before using If PPE becomes torn or defective remove and replace Remove PPE before leaving a contaminated area Contaminated PPE should be removed and disposed of in biohazard containers Do not reuse disposable equipment

24 (Facilities and Equipment that enhance safety)
Engineering Controls (Facilities and Equipment that enhance safety) HVAC negative pressure Fume hoods Use with volatile chemicals or non-infectious substances that pose an aerosol risk Toxic powders Note: The use of biological agents in a fume hood is prohibited. Use Biosafety cabinets with BSL-2 (or higher) agents where an aerosol hazard exists Electrical Protection, GFI Sound cabinets sonicators

25 Biosafety Cabinets General Operation Types Class I- no longer used Class II type A/B3- 70% recirculation, 30% exhaust into room (type A); or thimble connected to building HVAC- negative pressure plenum (type B3) Class II type B1- Cabinet air is 40% recirculated, hard ducted to HVAC, can be used with minute amounts of chemicals. Class II type B2- 100% exhaust can be used with small amounts of chemicals, plenum is totally under negative pressure. Class III- glovebox Must be certified at least annually- PI responsibility

26 Fume Hoods Do not store chemicals or equipment in fume hoods
Use appropriate PPE Use with sash in proper position Check for proper airflow before using (e.g., “tissue on sash alarm”) Report any diminished airflow to Building Manager ( )

27 Lab Specific Safety Training
The Lab supervisor/PI is required to provide lab specific safety training to staff working in their in their lab(s) Laboratory Specific training should supplement general training on laboratory specific hazards and safety procedures Additional specific training (radiation safety, animal handling, etc.) may also be required NOTE: The PI is responsible for safety in his/her laboratory

28 OSHA BloodBorne Pathogen Standard

29 Your Exposure Potential
Laboratory accidents Sharps Spills [Animal exposure (LAF Occupational Exposure Medical Plan)]* Handling of human (animal*) samples Handling of any waste products First aid administration Post-accident cleanup Janitorial or maintenance work *Animal tissues and fluids are not included in the official OSHA BloodBorne Pathogen Standard, but pose similar risks and are thus included in this discussion

30 Common Bloodborne Pathogens
Hepatitis B(HBV) Hepatitis C(HCV) Human Immunodeficiency Virus (HIV)

31 Potentially Infectious Substances
Human (and Animal*) Blood Skin and tissue Cell cultures Saliva Vomit Urine Semen and vaginal secretions Any bodily fluid or substance

32 Universal Precautions
Treat all blood and bodily fluids and samples as if they are contaminated Proper cleanup and decontamination Dispose of contaminated materials in the proper biohazard containers Use of proper PPE

33 Hand Washing Wash hands immediately after removing PPE and before leaving laboratory Use a soft antibacterial soap Do not use bleach A hand sanitizer can be used, but wash with soap and water as soon as possible.

34 Medical Program Vaccinations Animal Handling*
Hepatitis B Animal Handling* (LAF Occupational Exposure Medical Plan) Exotic agents

35 Exposure Incident OSHA requirement: Each lab should have a written, lab specific Exposure Control Plan (template available at EH&S website) An exposure is a specific incident of contact with potentially infectious bodily fluid If there are no infiltrations of mucous membranes or open skin surfaces, it is not considered an occupational exposure Report all accidents involving blood or bodily fluids to your superior and UB EH&S Post-exposure medical evaluations must be offered (personal physician or clinic)

36 Post-Exposure Evaluation
Confidential medical evaluation Document route of exposure Identify source individual Test source individual’s blood (with individual’s consent) Provide results to exposed employee File C2 workers accident form with NYS Workers Compensation Board ( )

37 Hepatitis B Vaccination
Strongly endorsed by medical communities Offered to all employees working with bloodborne hazards- must be documented Provided at no cost to employees Declination form

38 Decontamination Wear appropriate PPE
When decontaminating surfaces use appropriate disinfectant Cover contaminated area with disinfectant, allow appropriate contact time, and wipe up Dispose of all wipes in biohazard containers

39 Chemical Decontamination
Know the proper decontaminant and proper usage for each agent Common Examples: Bleach (sodium hypochlorite) 1:10 dilution 5.25% household bleach (5,000 ppm free chlorine) 30 minute exposure time 2 week shelf life (diluted) 6 month shelf life (undiluted) Corrosive to metal Ethanol 70% Rapidly bactericidal Noncorrosive Flammable Not sporicidal Unable to kill hydrophilic viruses May increase latex permeability to viruses

40 Autoclave Use Operation Validation 121o C for a minimum of 20 minutes.
Bags should be no more than 2/3 full Bags should not be completely sealed during autoclaving Bags should be placed in a container capable of containing any contents that may leak from them  Validation An indicator must be present in each load (autoclave tape, steam strip, spore test)

41 Laboratory Door Posting
Required on each research lab door Quick reference in case of an emergency or an issue concerning safety Fillable order form available on EH&S site: buffalo.org/Departments/ehs/ EHSForms Reviewed and updated annually or whenever a significant change takes place

42 Lab Security Report any suspicious individuals immediately to Kaleida Security ( ) Report any lost, missing, or stolen hazardous materials (biologicals, chemicals, radioactive materials, etc.) to EH&S ( ) Report any lost, stolen, or found keys, or any failures of the security doors immediately to Kaleida Security and the CTRC Manager ( )

43 Lab Security Security is only as strong as the occupants wish it to be
No tailgating (one swipe card = one person in) Doors should not be propped open If someone looks out of place, ask if they need assistance Do not leave valuables in the open Lock doors when rooms are vacant

44 Chemical Safety

45 Regulatory Agencies for Hazardous Chemicals
Occupational Safety and Health Administration (OSHA) [Workplace] New York State Department of Labor (DOL) [private firms] Public Employee Safety and Health Bureau (PESH)- NYS employees National Fire Protection Association (NFPA) [Fire Protection and Storage]

46 Agencies that Regulate Hazardous Waste
Hazardous waste generators must comply with regulations enforced by these agencies: Environmental Protection Agency (EPA) New York State Department of Environmental Conservation (NYSDEC) Department of Transportation (DOT)

47 Flammable Chemicals Flash point <100°F (Combustible - Flash Point > °F) Fire/Explosion Hazard Keep Sparks and Flames Away Examples Acetone, Ethanol, Methanol

48 Reactive Chemicals Release Large Amounts of Energy
React Violently with Water or Air React with Other Chemicals to Produce Toxic Gases Rapid Pressure Build-up/Explosion Potential Unstable/Readily Undergoes Change Examples: Calcium Hydride, Sodium Metal, and Organic Peroxides

49 Corrosive Chemicals Acids or Alkalis (Bases) Destructive to Tissue
Generates Heat During Reactions Examples: Hydrochloric Acid, Potassium Hydroxide, Hydrofluoric acid

50 Sensitizer Chemicals Allergic Reaction
Repeated exposure may worsen reaction Individuals React Differently! Severity Depends on Sensitivity, Potency, Concentration, and Duration Examples: Poison Ivy, Chromic Acid, Nickel

51 How Chemicals Enter The Body
Inhalation Skin Contact Dermal Absorption Mucosal surfaces Breaks in the Skin Ingestion Injection (Sharps)

52 Target Organs Hepatotoxins - Liver Nephrotoxins - Kidney Lungs
Teratogen - Reproductive Toxins Mutagen - Cellular Blood and Lymph System Immune System Neurotoxins- Nervous System

53 Symptoms of Exposure Eye discomfort Breathing difficulty Dizziness
Headache Nausea Vomiting Skin irritation

54 Acute Vs. Chronic Illness
Short-term Exposure Immediate or slightly delayed health effects Chronic Long-term Exposure Delayed effects

55 The Dose Determines the Poison
ONE YEAR 1 LB. ONE HOUR 1 LB.

56 How to Control Hazards Risk assessment Recognize hazards
Evaluate and minimize risks Control hazards Hazard Category1 Frequency Catastrophic Critical Marginal Negligible Frequent 1 3 7 13 Probable 2 5 9 16 Occasional 4 6 11 18 Remote 8 10 14 19 Improbable 12 15 17 20 Hazard Risk Index Review Criteria 1-5 Unacceptable 6-9 Undesirable 10-17 Acceptable with Review by Biosafety Officer 18-20 Acceptable without review

57 Hazard Awareness Signs Labels Tags Training Plan ahead Experience

58 Hazard Awareness Chemical Inventory (required) Information on Hazards
MSDS (required in every lab) Note that MSDS’ are being transitioned to Safety Data Sheets (SDSs) from June-December 2015 MSDs ≠ SDSs Internet Literature

59 Safety Data Sheets (formerly MSDS’)
Cannot take an MSDS and call it an SDS! 16 specific sections, must be in order Sections not being enforced Include Tox/Disposal/Transport/Reg. Info Outside OSHA jurisdiction May be paper or electronic Provide in English or other languages

60 SDS Sections Sec. 1: Identification; Sec. 2: Hazard identification;
Sec. 3: Composition/information on ingredients; Sec. 4: First aid measures; Sec. 5: Fire-fighting measures; Sec. 6: Accidental release measures; Sec. 7: Handling and storage; Sec. 8: Exposure control/personal protection; Sec. 9: Physical and chemical properties; Sec. 10: Stability and reactivity; Sec. 11: Toxicological information; Sec. 12*: Ecological information; Sec. 13*: Disposal considerations; Sec. 14*: Transport information; Sec. 15*: Regulatory information; and Sec. 16: Other information, including date of preparation or most recent revision.

61 New Labeling Requirements with HAZCOM Update
HCS Pictograms and Hazards Health Hazard Carcinogen Mutagenicity Reproductive Toxicity Respiratory Sensitizer Target Organ Toxicity Aspiration Toxicity Flame Flammables Pyrophorics Self-Heating Emits Flammable Gas Self-Reactives Organic Peroxides Exclamation Mark Irritant (skin and eye) Skin Sensitizer Acute Toxicity Narcotic Effects Respiratory Tract Irritant Hazardous to Ozone Layer (Non-Mandatory) Gas Cylinder Gases Under Pressure Corrosion Skin Corrosion/Burns Eye Damage Corrosive to Metals Exploding Bomb Explosives Flame Over Circle Oxidizers Environment (Non-Mandatory) Aquatic Toxicity Skull and Crossbones Acute Toxicity (fatal or toxic)

62 Labels Symbols (Pictograms)
Signal words “Danger” or Warning” – emphasize hazards, level of severity Hazard Statements – standard phrases Precautionary Statements

63 Hazard Control - Administrative Controls
Chemical Hygiene Plan (available from EH&S) Written Policies and SOPs Emergency Procedures After Hours Policy – No Working Alone

64 Safe Chemical Handling
Keep Containers Closed When Not in Use Avoid Contact with Incompatible Materials Only Transfer to Approved Containers Clean Up Spills, Dispose of Waste Properly Label Containers

65 Safe Handling of Flammable Chemicals
Store Chemicals Properly Bond (Ground) All Receiving Containers Store Quantities in Approved Storage Rooms and Cabinets Keep Away from Ignition Sources

66 Hazardous Spills

67 Hazardous Spill Response
Please remember: Do not attempt to clean up any hazardous spill yourself unless you are properly trained and have the capability to do so! Notify staff in the immediate area and the appropriate safety staff and post the area with signs alerting people of the spill Contain spill if possible Biological or Chemical: Evacuate lab Radioactive: Stay at lab doorway Wait for instructions

68 Biological or Chemical Spill
Remove any contaminated clothing or personal protective equipment (PPE)- do not track the spill If necessary, use emergency shower or eyewash Contain spill if possible Each laboratory is responsible for maintaining spill kits that address their specific hazards (biological, acids, bases, formaldehyde and solvents plus general sorbents (Available from EH&S $45, replenish at no cost) Call EH&S at during working hours Call Kaleida Security ( ) after hours Remain near lab for instructions and to provide information when proper response personnel arrive

69 Large or Small Spill? Large Spills Small Spills Response
Greater than 1 liter Mercury greater than amount in a standard thermometer Response Evacuate Area Close doors to prevent people from entering Call for assistance Secure area until proper response personnel arrive Small Spills Remove people from area If anyone requires first aid, see to them first Isolate/secure the spill area Proceed to clean up with spill kit Dispose of as hazardous waste

70 Radioactive Spill Remove any contaminated clothing & PPE. Wash contaminated skin with warm soapy water. Notify staff and post the area of the spill and contact Radiation Safety ( ) Contain and/or shield spill if possible Stay at lab door until monitored for contamination. Note: Additional training is required to work with radioactive materials or radiation generating equipment.

71 Waste Disposal

72 Waste Disposal Procedures
Biological Waste: Stericycle is the waste disposal provider at CTRC- EACH LAB MUST SET UP AN ACCOUNT Line biohazard box with red bag (rm 6068 and 6015) Place all non-sharp biohazardous waste into red bag Use plastic sharps containers for all sharps Place sealed full sharps containers into red bag lined box and indicate sharps on the outer label Seal full boxes with packing tape Place account sticker on outside of box Boxes should not weigh over 50 pounds Boxes can be transported to room 6015 for pickup

73 Sharps Disposal Procedures
Lined multi-ply cardboard box for uncontaminated glass Properly labeled heavy gauge plastic sharps container for contaminated sharps Needles, scalpels, etc. Do not clip or recap needles Put in sharps container in lab Broken glassware Use tongs or broom and shovel to pick up

74 Common Hazardous Waste Myths
“It’s Not a Waste Until I Say It’s a Waste” “I Can Just React It and Pour It Down the Sink” “Training and Records Aren’t a High Priority” “Just put the bottle in the fume hood and take the cap off…” “The solution to pollution is dilution”

75 A Container That Held Any Hazardous Waste Is RCRA Empty If :
RCRA Empty Containers A Container That Held Any Hazardous Waste Is RCRA Empty If : All Wastes Have Been Removed That Can Be Removed For Acutely Hazardous Wastes - The container IS hazardous waste, OR Container has been triple rinsed using an appropriate solvent and rinsate is collected for proper disposal

76 Disposal of Empty Containers
To show that the empty container no longer contains hazardous materials: Remove the Label or Completely Deface It with a Marker or Tape Over the Label, and … Place a “RCRA Empty” Label on the container:

77 US EPA Definition – Hazardous Waste
Is the Material a Hazardous Waste? On a US EPA List or: Fits Hazardous Waste Definition Ignitable Corrosive Toxic Reactive

78 Do not use chemical symbols, abbreviations, or formulas
Waste Labeling Instructions Front Back (Peel and Stick) Do not use chemical symbols, abbreviations, or formulas

79 Waste Disposal Procedures
Hazardous Chemical Waste: Collect waste in appropriately labeled container (labels available from CTRC Manager and EH&S) Containers must be capped Containers undamaged, free of leaks and spills Containers properly stored within secondary containment Incompatible wastes segregated in separate secondary containment Base under containers in good condition Download “Request for Hazardous Waste Disposal” electronic form on UB EH&S website, fill in by hand and fax or a scanned copy to EH&S Questions can be directed to UB EH&S ( )

80 Satellite Accumulation Area Inspection - Perform Weekly
Are containers: Labeled? Capped? Undamaged, free of leaks and spills? Properly stored within secondary containment? Incompatible wastes segregated in separate secondary containment? Base under containers in good condition? Keep Inspection Records for 3 Years

81 Hazardous Waste Guidebook
Hazardous Waste Guidebook Resource is there for you. Use it! Available on line at or from the EH&S office at

82 Waste Disposal Procedures
Radioactive Waste: Collect in appropriate labeled & shielded (i.e., if needed) container Separate containers for: Type (e.g., dry, liquid, scintillation fluid) Nuclide (e.g., H-3, I-125, P-32) Call Radiation Safety ( ) for instructions Note: Additional training is required to work with radioactive materials or radiation generating equipment

83 Disposal of Empty Containers
Under no circumstances may a container labeled with the international radioactive symbol, biohazard symbol or with the words “hazardous waste” be disposed of in the regular trash Label must be removed or defaced

84 Injury Major- Go to BGMC Emergency Department or call Kaleida Security ( ) Minor- Notify supervisor and go to BGMC Emergency Department or personal physician AEDs and first aid kits are located on each floor near kitchenette/conference rooms- know where they are. First aid can only be performed by individuals who have proper training

85 Documentation Biological Hygiene Plan- under revision
Organization and administration General operating procedures General safety procedures Personal protective equipment Emergency response Chemical Hygiene Plan Procedure Specific SOPs Employee Records Employee right to know Privacy

86 Emergency plans Spills Emergency contacts Fire evacuation route
CTRC- Kaleida Security EH&S Fire evacuation route Exposures

87 References UB Environment, Health, and Safety: buffalo.org/Departments/ehs OSHA, EPA, Safety in Academic Chemistry Laboratories (American Chemical Society) Biosafety in Microbiological and Biomedical Laboratories- Fifth edition: American Biological Safety Association: OSHA Bloodborne Pathogen Standard: DS&p_id=10051 APIC guidelines for disinfectant use (Amer. J Inf. Contr., vol 24, No. 4, pp , August 1996) Antiseptics and disinfectants: activity, action, and resistance (Clin Mic Rev, Jan , p. 147–179 Vol. 12, No. 1) Health Canada MSDLs:


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