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* Provide Required Annual Safety Training for Personnel at the CTRC.

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Presentation on theme: "* Provide Required Annual Safety Training for Personnel at the CTRC."— Presentation transcript:



3 * Provide Required Annual Safety Training for Personnel at the CTRC

4  Personnel and Responsibilities * CTRC Director- Dr Timothy Murphy * CTRC Manager- Dr. Richard Karalus * UB Biosafety Officer- David Pawlowski, Ph.D * UB Radiation Safety Officer- Jeff Slawson * Environmental Programs Manager- Brian Foti * Chemical Hygiene Officer, Hazardous Materials Manager- Anthony Oswald * Environmental Health and Safety Services 829-3301 * Employer (PI) responsibilities * Employee responsibilities * PIs bear full responsibility for safety in their laboratories

5  Fire Safety

6  During a fire  Turn off oxygen, gas, and electrical equipment in the affected area  USE THE STAIRS – NEVER USE THE ELEVATORS  Know primary and secondary evacuation routes

7 * Bunsen Burners/open flame devices should be used only when necessary and should always be attended. * Consider bacticinerators and micro burners as alternatives to Bunsen Burners. * Biosafety Cabinets - open flames are NOT recommended. They can damage the HEPA filter and cause a fire.

8 * Extension cords are not permitted for permanent applications * Space heaters must be equipped with tip over shutoff devices * Daisy Chained power strips are not permitted * Maintain a minimum of 18" between boxes and ceiling

9  Safety Equipment * Fire alarm pull stations * Each stairwell entrance * CRC * Fire extinguishers * Emergency showers * Emergency eye wash stations * AEDs near SW corner of each floor * First aid kits Know locations of safety equipment

10 * Fire alarm pull stations are located by each stair well * Fire alarms will include both strobe and audio alarms * CTRC alarms only alarm floor involved and the adjacent floors * Only alarming floors are required to evacuate unless otherwise advised * Fire Safety

11 * Fire Extinguishers: * Located in most laboratories * Do not obstruct or conceal fire extinguishers * Located in hallways throughout CTRC * Know where your nearest fire extinguisher is

12 * When using remember P.A.S.S. P = Pull the pin A = Aim at base of fire about 8-10ft away S = Squeeze the trigger S = Sweep side to side * “How to Use” instructions can be found on the fire extinguisher label * USE ONLY IF FIRE IS SMALL AND IF YOU HAVE HAD TRAINING ON ITS USE

13 R = R escue R escue people in the immediate area A = A nnounce A nnounce the fire verbally A ctivate the alarm C = C onfine C onfine fire by C losing doors E = E vacuate E vacuate the floor, E xtinguish if a small fire








21  Biosafety levels * BSL-1 * Organisms that do not normally cause human infections * BSL-2 * Organisms that cause human infections of low morbidity/mortality * Potential for aerosol transmission * BSL-3 * Organisms that cause human infections of high morbidity/mortality * Aerosol transmission * BSL-4 * Organisms of extremely high morbidity/mortality for which there are no treatments * Aerosol or unknown transmission

22  General Safety * General laboratory rules * Eating, drinking, smoking or the use of other tobacco products or cosmetics is strictly prohibited * The application or removal of contact lenses is forbidden * Storage of these items in the laboratory is prohibited * Mouth pipetting is strictly prohibited * Open toed shoes are not permitted * Minimize aerosols * Wash Hands

23  Personal Protective Equipment * Should never serve as primary protection * Appropriate for risk * Minimum * Disposable surgical gloves * Nitrile (recommended) * Latex * Eye protection * Splash * UV/other radiation * Lab Coat * Respirators (may require fit testing and medical clearance- contact EH&S) * Particulate (N95, PAPR, P-100) * Chemical cartridge (activated charcoal, chlorine, etc) * Hearing

24 * PPE Rules to Remember  Always check PPE for defects or tears before using  If PPE becomes torn or defective remove and replace  Remove PPE before leaving a contaminated area  Contaminated PPE should be removed and disposed of in biohazard containers  Do not reuse disposable equipment

25  Engineering Controls (Facilities and Equipment that enhance safety) * HVAC negative pressure * Fume hoods * Use with volatile chemicals or non-infectious substances that pose an aerosol risk * Toxic powders Note: The use of biological agents in a fume hood is prohibited. * Use Biosafety cabinets with BSL-2 (or higher) agents where an aerosol hazard exists * Electrical Protection, * GFI * Sound cabinets * sonicators

26  Biosafety Cabinets * General Operation * Types * Class I- no longer used * Class II type A/B3- 70% recirculation, 30% exhaust into room (type A); or thimble connected to building HVAC- negative pressure plenum (type B3) * Class II type B1- Cabinet air is 40% recirculated, hard ducted to HVAC, can be used with minute amounts of chemicals. * Class II type B2- 100% exhaust can be used with small amounts of chemicals, plenum is totally under negative pressure. * Class III- glovebox * Must be certified at least annually- PI responsibility

27  Do not store chemicals or equipment in fume hoods  Use appropriate PPE  Use with sash in proper position  Check for proper airflow before using (e.g., “tissue on sash alarm”)  Report any diminished airflow to Building Manager (888-4730)

28 * The Lab supervisor/PI is required to provide lab specific safety training to staff working in their in their lab(s) * Laboratory Specific training should supplement general training on laboratory specific hazards and safety procedures * Additional specific training (radiation safety, animal handling, etc.) may also be required NOTE: The PI is responsible for safety in his/her laboratory


30 * Your Exposure Potential  Laboratory accidents  Sharps  Spills  [Animal exposure (LAF Occupational Exposure Medical Plan)] *  Handling of human (animal * ) samples  Handling of any waste products  First aid administration  Post-accident cleanup  Janitorial or maintenance work * Animal tissues and fluids are not included in the official OSHA BloodBorne Pathogen Standard, but pose similar risks and are thus included in this discussion

31  Common Bloodborne Pathogens * Hepatitis B(HBV) * Hepatitis C(HCV) * Human Immunodeficiency Virus (HIV)

32  Potentially Infectious Substances * Human (and Animal * ) * Blood * Skin and tissue * Cell cultures * Saliva * Vomit * Urine * Semen and vaginal secretions * Any bodily fluid or substance

33  Treat all blood and bodily fluids and samples as if they are contaminated  Proper cleanup and decontamination  Dispose of contaminated materials in the proper biohazard containers  Use of proper PPE

34  Wash hands immediately after removing PPE and before leaving laboratory  Use a soft antibacterial soap  Do not use bleach  A hand sanitizer can be used, but wash with soap and water as soon as possible.

35 * Vaccinations * Hepatitis B * Animal Handling* * (LAF Occupational Exposure Medical Plan) * Exotic agents

36  OSHA requirement: Each lab should have a written, lab specific Exposure Control Plan (template available at EH&S website)  An exposure is a specific incident of contact with potentially infectious bodily fluid  If there are no infiltrations of mucous membranes or open skin surfaces, it is not considered an occupational exposure  Report all accidents involving blood or bodily fluids to your superior and UB EH&S  Post-exposure medical evaluations must be offered (personal physician or clinic)

37  Confidential medical evaluation  Document route of exposure  Identify source individual  Test source individual’s blood (with individual’s consent)  Provide results to exposed employee  File C2 workers accident form with NYS Workers Compensation Board (1-866- 396-8314)

38  Strongly endorsed by medical communities  Offered to all employees working with bloodborne hazards- must be documented  Provided at no cost to employees  Declination form

39  Wear appropriate PPE  When decontaminating surfaces use appropriate disinfectant  Cover contaminated area with disinfectant, allow appropriate contact time, and wipe up  Dispose of all wipes in biohazard containers

40 * Know the proper decontaminant and proper usage for each agent Common Examples: * Bleach (sodium hypochlorite) * 1:10 dilution 5.25% household bleach (5,000 ppm free chlorine) * 30 minute exposure time * 2 week shelf life (diluted) * 6 month shelf life (undiluted) * Corrosive to metal * Ethanol * 70% * Rapidly bactericidal * Noncorrosive * Flammable * Not sporicidal * Unable to kill hydrophilic viruses * May increase latex permeability to viruses

41 * Operation * 121 o C for a minimum of 20 minutes. * Bags should be no more than 2/3 full * Bags should not be completely sealed during autoclaving * Bags should be placed in a container capable of containing any contents that may leak from them * Validation * An indicator must be present in each load (autoclave tape, steam strip, spore test)

42 * Required on each research lab door * Quick reference in case of an emergency or an issue concerning safety * Fillable order form available on EH&S site: http://www.facilities- EHSForms http://www.facilities- EHSForms * Reviewed and updated annually or whenever a significant change takes place

43 * Report any suspicious individuals immediately to Kaleida Security (859- 2196) * Report any lost, missing, or stolen hazardous materials (biologicals, chemicals, radioactive materials, etc.) to EH&S (829-3301) * Report any lost, stolen, or found keys, or any failures of the security doors immediately to Kaleida Security and the CTRC Manager (888-4730)

44 Security is only as strong as the occupants wish it to be * No tailgating (one swipe card = one person in) * Doors should not be propped open * If someone looks out of place, ask if they need assistance * Do not leave valuables in the open * Lock doors when rooms are vacant


46 * Occupational Safety and Health Administration (OSHA) [Workplace] * New York State Department of Labor (DOL) [private firms] * Public Employee Safety and Health Bureau (PESH)- NYS employees * National Fire Protection Association (NFPA) [Fire Protection and Storage]

47 * Environmental Protection Agency (EPA) * New York State Department of Environmental Conservation (NYSDEC) * Department of Transportation (DOT) Hazardous waste generators must comply with regulations enforced by these agencies:

48 * Flash point 100-200°F) * Fire/Explosion Hazard * Keep Sparks and Flames Away * Examples * Acetone, Ethanol, Methanol

49 * Release Large Amounts of Energy * React Violently with Water or Air * React with Other Chemicals to Produce Toxic Gases * Rapid Pressure Build-up/Explosion Potential * Unstable/Readily Undergoes Change * Examples: * Calcium Hydride, Sodium Metal, and Organic Peroxides

50 * Acids or Alkalis (Bases) * Destructive to Tissue * Generates Heat During Reactions * Examples: * Hydrochloric Acid, Potassium Hydroxide, Hydrofluoric acid

51 * Allergic Reaction * Repeated exposure may worsen reaction * Individuals React Differently! * Severity Depends on Sensitivity, Potency, Concentration, and Duration * Examples: * Poison Ivy, Chromic Acid, Nickel

52 * Inhalation * Skin Contact * Dermal Absorption * Mucosal surfaces * Breaks in the Skin * Ingestion * Injection (Sharps)

53 * Hepatotoxins - Liver * Nephrotoxins - Kidney * Lungs * Teratogen - Reproductive Toxins * Mutagen - Cellular * Blood and Lymph System * Immune System * Neurotoxins- Nervous System

54 * Eye discomfort * Breathing difficulty * Dizziness * Headache * Nausea * Vomiting * Skin irritation

55 * Acute * Short-term Exposure * Immediate or slightly delayed health effects * Chronic * Long-term Exposure * Delayed effects


57  Risk assessment  Recognize hazards  Evaluate and minimize risks  Control hazards Hazard Category 1 FrequencyCatastrophicCriticalMarginalNegligible Frequent13713 Probable25916 Occasional461118 Remote8101419 Improbable12151720 Hazard Risk IndexReview Criteria 1-5Unacceptable 6-9Undesirable 10-17Acceptable with Review by Biosafety Officer 18-20Acceptable without review

58 * Signs * Labels * Tags * Training * Plan ahead * Experience

59 * Chemical Inventory (required) * Information on Hazards * MSDS (required in every lab) * Note that MSDS’ are being transitioned to Safety Data Sheets (SDSs) from June-December 2015 * MSDs ≠ SDSs * Internet * Literature

60 * Cannot take an MSDS and call it an SDS! * 16 specific sections, must be in order * Sections 12-15 not being enforced * Include Tox/Disposal/Transport/Reg. Info * Outside OSHA jurisdiction * May be paper or electronic * Provide in English or other languages

61 * Sec. 1: Identification; * Sec. 2: Hazard identification; * Sec. 3: Composition/information on ingredients; * Sec. 4: First aid measures; * Sec. 5: Fire-fighting measures; * Sec. 6: Accidental release measures; * Sec. 7: Handling and storage; * Sec. 8: Exposure control/personal protection; * Sec. 9: Physical and chemical properties; * Sec. 10: Stability and reactivity; * Sec. 11: Toxicological information; * Sec. 12*: Ecological information; * Sec. 13*: Disposal considerations; * Sec. 14*: Transport information; * Sec. 15*: Regulatory information; and * Sec. 16: Other information, including date of preparation or most recent revision.

62 HCS Pictograms and Hazards Health Hazard Carcinogen Mutagenicity Reproductive Toxicity Respiratory Sensitizer Target Organ Toxicity Aspiration Toxicity Flame Flammables Pyrophorics Self-Heating Emits Flammable Gas Self-Reactives Organic Peroxides Exclamation Mark Irritant (skin and eye) Skin Sensitizer Acute Toxicity Narcotic Effects Respiratory Tract Irritant Hazardous to Ozone Layer (Non-Mandatory) Gas Cylinder Gases Under Pressure Corrosion Skin Corrosion/Burns Eye Damage Corrosive to Metals Exploding Bomb Explosives Self-Reactives Organic Peroxides Flame Over Circle Oxidizers Environment (Non-Mandatory) Aquatic Toxicity Skull and Crossbones Acute Toxicity (fatal or toxic)

63 * Symbols (Pictograms) * Signal words “Danger” or Warning” – emphasize hazards, level of severity * Hazard Statements – standard phrases * Precautionary Statements

64 * Chemical Hygiene Plan (available from EH&S) * Written Policies and SOPs * Emergency Procedures * After Hours Policy – No Working Alone

65 * Keep Containers Closed When Not in Use * Avoid Contact with Incompatible Materials * Only Transfer to Approved Containers * Clean Up Spills, Dispose of Waste Properly * Label Containers

66 * Store Chemicals Properly * Bond (Ground) All Receiving Containers * Store Quantities in Approved Storage Rooms and Cabinets * Keep Away from Ignition Sources


68 Please remember: * Do not attempt to clean up any hazardous spill yourself unless you are properly trained and have the capability to do so! * Notify staff in the immediate area and the appropriate safety staff and post the area with signs alerting people of the spill * Contain spill if possible * Biological or Chemical: Evacuate lab Radioactive: Stay at lab doorway * Wait for instructions

69 * Remove any contaminated clothing or personal protective equipment (PPE)- do not track the spill * If necessary, use emergency shower or eyewash * Contain spill if possible * Each laboratory is responsible for maintaining spill kits that address their specific hazards (biological, acids, bases, formaldehyde and solvents plus general sorbents (Available from EH&S $45, replenish at no cost) * Call EH&S at 829-3301 during working hours * Call Kaleida Security (859-2196) after hours * Remain near lab for instructions and to provide information when proper response personnel arrive

70 * Large Spills * Greater than 1 liter * Mercury greater than amount in a standard thermometer * Response * Evacuate Area * Close doors to prevent people from entering * Call for assistance * Secure area until proper response personnel arrive * Small Spills * Remove people from area * If anyone requires first aid, see to them first * Isolate/secure the spill area * Proceed to clean up with spill kit * Dispose of as hazardous waste

71 * Remove any contaminated clothing & PPE. Wash contaminated skin with warm soapy water. * Notify staff and post the area of the spill and contact Radiation Safety (829-3281) * Contain and/or shield spill if possible * Stay at lab door until monitored for contamination. Note: Additional training is required to work with radioactive materials or radiation generating equipment.


73 Biological Waste: * Stericycle is the waste disposal provider at CTRC- EACH LAB MUST SET UP AN ACCOUNT red bag * Line biohazard box with red bag (rm 6068 and 6015) * Place all non-sharp biohazardous waste into red bag sharps containers * Use plastic sharps containers for all sharps * Place sealed full sharps containers into red bag lined box and indicate sharps on the outer label * Seal full boxes with packing tape * Place account sticker on outside of box * Boxes should not weigh over 50 pounds * Boxes can be transported to room 6015 for pickup

74  Lined multi-ply cardboard box for uncontaminated glass  Properly labeled heavy gauge plastic sharps container for contaminated sharps  Needles, scalpels, etc.  Do not clip or recap needles  Put in sharps container in lab  Broken glassware  Use tongs or broom and shovel to pick up

75 * “It’s Not a Waste Until I Say It’s a Waste” * “I Can Just React It and Pour It Down the Sink” * “Training and Records Aren’t a High Priority” * “Just put the bottle in the fume hood and take the cap off…” * “The solution to pollution is dilution”

76 * The container IS hazardous waste, OR * Container has been triple rinsed using an appropriate solvent and rinsate is collected for proper disposal A Container That Held Any Hazardous Waste Is RCRA Empty If : All Wastes Have Been Removed That Can Be Removed For Acutely Hazardous Wastes -

77 or  Remove the Label or or  Completely Deface It with a Marker or and …  Tape Over the Label, and …  Place a “RCRA Empty” Label on the container: To show that the empty container no longer contains hazardous materials:

78 * On a US EPA List or: * Fits Hazardous Waste Definition * Ignitable * Corrosive * Toxic * Reactive Is the Material a Hazardous Waste?

79 Front Back (Peel and Stick)

80 Hazardous Chemical Waste:  Collect waste in appropriately labeled container (labels available from CTRC Manager and EH&S)  Containers must be capped  Containers undamaged, free of leaks and spills  Containers properly stored within secondary containment  Incompatible wastes segregated in separate secondary containment  Base under containers in good condition  Download “Request for Hazardous Waste Disposal” electronic form on UB EH&S website, fill in by hand and fax or email a scanned copy to EH&S  Questions can be directed to UB EH&S (829-3301)

81 * Are containers: * Labeled? * Capped? * Undamaged, free of leaks and spills? * Properly stored within secondary containment? * Incompatible wastes segregated in separate secondary containment? * Base under containers in good condition? * Keep Inspection Records for 3 Years


83 Radioactive Waste:  Collect in appropriate labeled & shielded (i.e., if needed) container  Separate containers for:  Type (e.g., dry, liquid, scintillation fluid)  Nuclide (e.g., H-3, I-125, P-32)  Call Radiation Safety (829-3281) for instructions Note: Additional training is required to work with radioactive materials or radiation generating equipment

84 regular trash  Under no circumstances may a container labeled with the international radioactive symbol, biohazard symbol or with the words “hazardous waste” be disposed of in the regular trash  Label must be removed or defaced

85 * Major- Go to BGMC Emergency Department or call Kaleida Security (859- 2196) * Minor- Notify supervisor and go to BGMC Emergency Department or personal physician * AEDs and first aid kits are located on each floor near kitchenette/conference rooms- know where they are. * First aid can only be performed by individuals who have proper training

86 * Biological Hygiene Plan- under revision * Organization and administration * General operating procedures * General safety procedures * Personal protective equipment * Emergency response * Chemical Hygiene Plan * Procedure Specific SOPs * Employee Records * Employee right to know * Privacy

87 * Spills * Emergency contacts * CTRC- Kaleida Security- 859-2196 * EH&S- 829-3301 * Fire evacuation route * Exposures

88 * UB Environment, Health, and Safety: http://www.facilities- * OSHA, * EPA, * Safety in Academic Chemistry Laboratories (American Chemical Society) * Biosafety in Microbiological and Biomedical Laboratories- Fifth edition: * American Biological Safety Association: * OSHA Bloodborne Pathogen Standard: DS&p_id=10051 DS&p_id=10051 * APIC guidelines for disinfectant use (Amer. J Inf. Contr., vol 24, No. 4, pp313-342, August 1996) * Antiseptics and disinfectants: activity, action, and resistance (Clin Mic Rev, Jan. 1999, p. 147–179 Vol. 12, No. 1) * Health Canada MSDLs:

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