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Study of Certain Autoantibodies Production in Visceral Leishmaniasis Professor Dr. Baqur A. Sultan*Dep. of Microbiolgy, College of Medicine, Kufa University.

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Presentation on theme: "Study of Certain Autoantibodies Production in Visceral Leishmaniasis Professor Dr. Baqur A. Sultan*Dep. of Microbiolgy, College of Medicine, Kufa University."— Presentation transcript:

1 Study of Certain Autoantibodies Production in Visceral Leishmaniasis Professor Dr. Baqur A. Sultan*Dep. of Microbiolgy, College of Medicine, Kufa University Assist. Professor Dr. Mohammad A. Al-Faham*Dep. of Microbiolgy, College of Medicine, Baghdad University. Dr. Raad Abdul-Alameer Al-Asady*Dep. of Microbiolgy, College of Medicine, Baghdad University

2 Iraq is an endemic area for visceral leishmaniasis (VL) (kala-azar), which is caused Leishmania donovani. Patients with VL have markedly elevated immunoglobulin levels and associated with existence of autoantibodies against cellular and humoral components. This study aimed to investigate the occurrence of some autoantibodies {anti-cardiolipin antibody (aCL), rheumatoid factor (RF), anti-neutrophil cytoplasmic antibody (ANCA)} in Iraqi children with VL.

3 Patients, Materials & methods: - 135 children serologically proven visceral leishmaniasis (71 males and 64 females), whose ages ranged from 2 month to 12 years, with 36 healthy children were involved in this study. - (aCL) By enzyme-linked immunosorbent assay (ELISA), - (RF) By latex agglutination test (LAT) (ANCA) By IFAT -

4 ACLKALA-AZAR PATIENTS %≤10 U/ml Negative% >10 U/ml Positive No. 641636925<1 Age (year) 7014306201-2 808202102-3 10030033-4 75325144-6 X² = 2.25, df = 4, P = 0.6893 68.82231.21032 Se mir ura l Area 8016204 Rur al 60640410 Urb an X² = 1.45, df = 2, P = 0.4838 7144291862Total Table (1): Sex distribution of aCL seropositivity in VL cases and healthy controls.

5 Table (2): Age and community-based distribution of aCL seropositivity in VL cases. ẠCL %≤10 U/ml Negative%>10 U/m PositiveNo. 752425832Male VL cases 66.72033.31030Female Yates corrected X² = 0.2, df = 1, P = 0.6581 1001200 Male Healthy controls 91.7118.3112Female Yates corrected X² = 0.00, df = 1, P = 1.0000 7144291862Total VL cases 95.8234.2124Total controls Yates corrected X² = 4.85, df = 1, P = 0.0275

6 RHEUMATOID FACTOR (RF) %Negative%PositiveNo. 70.72929.31241Male VL case s 75.72824.3937Female Yates corrected X² = 0.6, df = 1, P = 0.8134 93.3146.7115Male Heal thy cont rols 1001500 Female Yates corrected X² = 6.05, df = 1, P = 0.0139 73.15726.92178Total VL cases 96.7293.3130Total controls Yates corrected X² = 6.05, df = 1, P = 0.0139 Table (3): Sex distribution of RF seropositivity in VL cases and healthy control

7 RHEUMATOID FACTOR (RF)KALA-AZAR PATIENTS %Negative% Pos itiv e No. 61.31938.71231<1 Age (year) 7218287251-2 84.61115.42132-3 10030033-4 10060064-6 X² = 6.4, df =4, P = 0.1715 72.53927.51140 Se mir ural Area 761924625 Rur al 69.2930.8413 Urb an X² = 0.44, df = 2, P = 0.8037 73.15726.92178 Tot al Table (4): Age and community-based distribution of RF seropositivity in VL cases.

8 ANCA PATTERN VL PATIENTSHEALTHY CONTROLS No. (28)%No. (18)% c-ANCA Positive310.700 Negative2589.318100 p-ANCA Positive0000 Negative2810018100 Total ANCA Positive310.700 Negative2589.318100 Table (5): ANCA seropositivity in VL cases and healthy controls according to staining pattern.

9 ANCA %Negative%PositiveNo. 88.91611.1218Male VL case s 909101 Female Yates corrected X² = 0.3, df = 1, P = 0.5847 1009009Male Health y control s 1009009Female 89.32510.7328Total VL cases 1001800 Total controls Yates corrected X² = 0.68, df = 1, P = 0.4046 Table (6): Sex distribution of ANCA seropositivity in VL cases and healthy controls.

10 ANCAKALA-AZAR PATIENTS %Negative%PositiveNo. 87.51412.5216<1 Age (year) 88.9811.1191-2 10020022-3 10010013-4 X² = 0.41, df = 3, P = 0.9371 85.71214.3214Semirural Area 909101 Rural 1004004Urban X² = 0.67, df = 2, P = 0.7146 89.32510.7328Total Table (7): Age and community-based distribution of ANCA seropositivity in VL cases.

11 Figure 1: c-ANCA staining pattern on neutrophil cells (40X).

12 Conclusion: There is a positive correlation between kala-azar and aCL or RF, but there is no correlation between certain age, sex and community of children with VL and aCL or RF. There is no correlation between VL and ANCA in this study.

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