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Study of Certain Autoantibodies Production in Visceral Leishmaniasis Professor Dr. Baqur A. Sultan*Dep. of Microbiolgy, College of Medicine, Kufa University.

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Presentation on theme: "Study of Certain Autoantibodies Production in Visceral Leishmaniasis Professor Dr. Baqur A. Sultan*Dep. of Microbiolgy, College of Medicine, Kufa University."— Presentation transcript:

1 Study of Certain Autoantibodies Production in Visceral Leishmaniasis Professor Dr. Baqur A. Sultan*Dep. of Microbiolgy, College of Medicine, Kufa University Assist. Professor Dr. Mohammad A. Al-Faham*Dep. of Microbiolgy, College of Medicine, Baghdad University. Dr. Raad Abdul-Alameer Al-Asady*Dep. of Microbiolgy, College of Medicine, Baghdad University

2 Iraq is an endemic area for visceral leishmaniasis (VL) (kala-azar), which is caused Leishmania donovani. Patients with VL have markedly elevated immunoglobulin levels and associated with existence of autoantibodies against cellular and humoral components. This study aimed to investigate the occurrence of some autoantibodies {anti-cardiolipin antibody (aCL), rheumatoid factor (RF), anti-neutrophil cytoplasmic antibody (ANCA)} in Iraqi children with VL.

3 Patients, Materials & methods: children serologically proven visceral leishmaniasis (71 males and 64 females), whose ages ranged from 2 month to 12 years, with 36 healthy children were involved in this study. - (aCL) By enzyme-linked immunosorbent assay (ELISA), - (RF) By latex agglutination test (LAT) (ANCA) By IFAT -

4 ACLKALA-AZAR PATIENTS %≤10 U/ml Negative% >10 U/ml Positive No <1 Age (year) X² = 2.25, df = 4, P = Se mir ura l Area Rur al Urb an X² = 1.45, df = 2, P = Total Table (1): Sex distribution of aCL seropositivity in VL cases and healthy controls.

5 Table (2): Age and community-based distribution of aCL seropositivity in VL cases. ẠCL %≤10 U/ml Negative%>10 U/m PositiveNo Male VL cases Female Yates corrected X² = 0.2, df = 1, P = Male Healthy controls Female Yates corrected X² = 0.00, df = 1, P = Total VL cases Total controls Yates corrected X² = 4.85, df = 1, P =

6 RHEUMATOID FACTOR (RF) %Negative%PositiveNo Male VL case s Female Yates corrected X² = 0.6, df = 1, P = Male Heal thy cont rols Female Yates corrected X² = 6.05, df = 1, P = Total VL cases Total controls Yates corrected X² = 6.05, df = 1, P = Table (3): Sex distribution of RF seropositivity in VL cases and healthy control

7 RHEUMATOID FACTOR (RF)KALA-AZAR PATIENTS %Negative% Pos itiv e No <1 Age (year) X² = 6.4, df =4, P = Se mir ural Area Rur al Urb an X² = 0.44, df = 2, P = Tot al Table (4): Age and community-based distribution of RF seropositivity in VL cases.

8 ANCA PATTERN VL PATIENTSHEALTHY CONTROLS No. (28)%No. (18)% c-ANCA Positive Negative p-ANCA Positive0000 Negative Total ANCA Positive Negative Table (5): ANCA seropositivity in VL cases and healthy controls according to staining pattern.

9 ANCA %Negative%PositiveNo Male VL case s Female Yates corrected X² = 0.3, df = 1, P = Male Health y control s Female Total VL cases Total controls Yates corrected X² = 0.68, df = 1, P = Table (6): Sex distribution of ANCA seropositivity in VL cases and healthy controls.

10 ANCAKALA-AZAR PATIENTS %Negative%PositiveNo <1 Age (year) X² = 0.41, df = 3, P = Semirural Area Rural Urban X² = 0.67, df = 2, P = Total Table (7): Age and community-based distribution of ANCA seropositivity in VL cases.

11 Figure 1: c-ANCA staining pattern on neutrophil cells (40X).

12 Conclusion: There is a positive correlation between kala-azar and aCL or RF, but there is no correlation between certain age, sex and community of children with VL and aCL or RF. There is no correlation between VL and ANCA in this study.

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