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Study of Influence of Salts Additives to Mixed Reverse Micelles via Reverse Microemulsion Chromatography and UV- visible spectroscopy Manuchar Gvaramia,

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Presentation on theme: "Study of Influence of Salts Additives to Mixed Reverse Micelles via Reverse Microemulsion Chromatography and UV- visible spectroscopy Manuchar Gvaramia,"— Presentation transcript:

1 Study of Influence of Salts Additives to Mixed Reverse Micelles via Reverse Microemulsion Chromatography and UV- visible spectroscopy Manuchar Gvaramia, Postgraduate student, Department of Chemistry, Faculty of Exact and Natural Sciences, Tbilisi University, Georgia

2 Reverse micelle Direct micelle

3 Mixed microemulsions on the basis of nonionic and anionic surfactants

4 Dependence of retention factors of solutes on the water/surfactant ratio

5

6 Water,Brij-30, butanol and hexane with ratio (v/v.%): 0.3/2.8/4.6/92.3

7 Water,Brij-30, butanol and hexane with ratio (v/v.%): 0.7/2.8/4.6/91.9

8 Diagram of retention factors of solutes at w=2( ) and w=3 (  ) in the presence of sodium acetate(0.025M)

9

10 Diagram of retention factors of solutes at w=2() and w=3(  ) in the presence of potassium perchlotate(0.025)

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12 Diagram of retention factors of solutes at the presense of sodium acetate () and potassium perchlorate(  ) W=2

13 O-nitroaniline as molecular probe

14 Determination of binding constants of o- nitroaniline (o-NA) to Brij-30 reverse micelles

15 Absorption spectra of o-NA in hexane/( ♦- 0.0M Brij M AOT, 0.13 ·10 -2 M Brij AOT, 0.91·10 -2 M Brij M AOT, 2.73·10 -2 M Brij M AOT, 11.7·10 -2 M Brij M AOT, 23.4·10 -2 M Brij M AOT) at Brij-30 concentrations. At W 0 =0, [o-NA]=4.5·10 -5 M

16 Variation of λ m of o-NA in reverse micelles as a function of Brij-30 concentration

17 Diagram of K b of o-NA with (Brij-30+AOT) mixed micelles in the following conditions:1) absence of water, 2) presence of water, 3) presence of 0.05M sodium acetate and 4) 0.05M potassium perchlorate

18 a) Diagram of retention factors of solutes in the presence of sodium acetate (■) and potassium perchlorate (□) W=2 b) Diagram of binding constants of o-NA with (Brij-30+AOT) mixed micelles in the presence of 0.05M sodium acetate(■) and 0.05M potassium perchlorate(□) a b

19 Conclusions Binding of o-nitroaniline to reverse micelles is higher in the presence of chaotropic perchlorate ions in comparison with kosmotropic acetate ions. Solubility of the model compounds is lower in the presence of chaotropic anions in the water pools of reverse micelles as compared with kosmotropic acetate ions. Retention factor of the solutes are higher in the presence of kosmotropic acetate ions in the water nanocages of the reverse micelles as compared to perchlorate ions.

20 Conclusions Results may be useful in the investigations of water structure, when it is confined to nanometer-scale cavities, viz. in biological systems.

21 Acknowlegement I thank the Georgian Shota Rustaveli National Science Foundation and Julich Scientific Center for opportunity to participate in this project

22 Thanks for attention

23

24 SO 4 2- > F - > OAc - > Cl - > Br - > NO 3 - > I - > ClO 4 - > SCN -

25 Benzene-1,3-diol resorcinol benzene-1,2,3-triol pyrogallol benzene-1,4-diol hydroquinone

26 1,3-dimethyl-7H-purine-2,6-dione theophylline 1,3,7-trimethyl-1H-purine-2,6(3H,7H)-dione caffeine

27 2,2,2-trichloroethane-1,1-diol Phenobarbital (5-ethyl-5-phenylbarbituric acid or 5-ethyl-5-phenylhexahydropyrimindin-2,4,6-trione)

28 carbamazepine

29 Absorption spectra of o-NA in different solvents:1) water (412), 2) ethanol (406 nm), 3) methanol (405nm) and 4)isopropanol (407 nm)

30 Possible scheme of arrangement of ions in water pools of the reverse micelle


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