1. Darwinian Genomics Csaba Pal Biological Research Center Szeged, Hungary

2. Genomics: Major revolution in the past 10 – 15 years with the rise of high-throughput molecular technologies: New methods for rapid and relatively cheap measurements of biological molecules on a global scale

3. Systematic mapping components, interactions and functional states of the cell Genomics: genome sequencing and annotation Transcriptomics: mRNA levels, mRNA half-lives Proteomics: protein levels, protein – protein interactions, protein modifications Metabolomics: metabolite concentrations Phenomics: creating collections of mutant strains and measuring phenotypes (e.g. cell growth) under various conditions

4. Darwinian genomics: Testing key issues in evolutionary biology Examples: 1)Role of chance and necessity 2)Gradual changes or jumps 3)Extent and evolution of robustness against mutations

5. Darwinian genomics: Testing key issues in evolutionary biology Examples: 1)Role of chance and necessity 2)Gradual changes or jumps 3)Extent and evolution of robustness against mutations

6. Yeast (S. cerevisiae) is an ideal model organism 1)Complete genome sequence/detailed biochemical studies -> network reconstruction 2) Genome-scale computational models -> systems level properties of cellular networks 3) Large-scale mutant libraries -> test predictions of the models 4) Complete genome sequences for ~30 closely related species -> study evolution across species

7. The knock-out paradox High-throughput single gene knock-out studies: no phenotype for most genes in the lab

8. Why keep them during evolution? 1)Keep optimal cellular performance in face of harmful mutations and non-heritable errors 2)Allow cellular growth under wide range of external conditions

9.  compensated by a gene duplicate (genetic redundancy)  compensated by alternative genetic pathways (distributed robustness)  have important functions only under specific environmental conditions (Seemingly) dispensable genes.... Gene A Gene B Gene A

10. Redundancy is only apparent, most genes should have important contribution to survival under special environmental conditions

11. Hillenmeyer et al. Science 2008

12. Compared growth rates of ~ 5000 single gene knock-out strains under >1000 environments 97% of the mutants show slow growth under at least one condition

13.  compensated by a gene duplicate (genetic redundancy)  compensated by alternative genetic pathways (distributed robustness)  have important functions only under specific environmental conditions Are these explanations mutually exclusive? Gene A Gene B Gene A

14. Does the capacity to compensate the impact of gene deletions depend on the environment?

15. A B A B A B A B Environment I. a B A b a b A B a B A b a b A B a B A b a b Environment II.Environment III. Observed gene deletion phenotypes ( viable, lethal): synthetic lethalityno interaction The extent of compensation may depend on nutrient availability

16. Computational tool: Flux Balance Analysis (FBA) 1)Network reconstruction In S. cerevisiae ~1400 biochemical reactions, including transport processes. 2)Application of constraints Specify the nutrients available in the environment (B,E), the key metabolites or biomass constituents (X, Y, Z) essential for survival, presence/absence of genes 3)Find a particular enzymatic flux distribution -> rate of biomass production (fitness) Amino acids Carbohydrates Ribonucleotides Deoxyribonucleotides Lipids Phospholipids Steroles Fatty acids fitness

17. What are the advantages of flux balance analysis? 1)Study large number of genes and environments simultaneously 2)Predictions: a) Changes in enzyme activity as a response to nutrient conditions and genetic deletions b) Impact of gene deletions and gene addition on growth rates 3) Good agreement between experimental studies and model predictions (~90%) Forster et al. 2003 OMICS, Papp et al. Nature 2004

18. Interactions between mutations in metabolic networks A special case: Synthetic lethal genetic interactions A B normal growth lethal (or sick) a – B A b – a – b – Redundant gene duplicates Gene A Gene B Gene A Alternative cellular pathways

19. Model predictions and verification of genetic interactions Using Flux Balance analysis, we simulated all possible single and double gene deletions (~125 000) in the metabolic network under 53 different nutrient conditions  98 gene pairs are synthetic lethal under at least one condition We performed lab experiments to validate them: ΔABΔAB n AΔBAΔB n A/ΔA B/ΔB 2n sporulation

20. Results: 1) 50% of the predictions were correct (only ~ 0.6% expected by chance!) 2) 85% of the interacting gene pairs show condition-dependent synthetic lethality unconditional synthetic lethality

21. Harrison et al. (2007) PNAS 104:2307-2312 An example:

22. Harrison et al. (2007) PNAS 104:2307-2312 An example:

23. Conclusions The metabolic network model can reliably predict (synthetic lethal) genetic interactions. The presence of genetic interactions (and hence the extent of compensation) vary extensively across nutrient conditions.

24. Speculations and potential implications: Experimental design. Different environments should be screened to identify the majority of genetic interactions Functional genomics. Redundancy is more apparent than real. Many seemingly dispensable genes have important physiological role under specific conditions Evolution. Robustness against mutations may not be a directly selected trait, but rather a by-product of evolution of novel metabolic pathways towards new environmental conditions

25. Shortcomings:  The computational model is far from perfect, and ignores many biological details  Only specific genetic interactions have been studied  No systematic experimental screen Harrison et al. (2007) PNAS 104:2307-2312

26. Collaboration with Charles Boone lab 1)Using robotic protocols, they map genetic interactions across the whole yeast genome (~10 7 combinations ) 2)They developed high-throughput protocols to measure fitness at high precision

27. Why study evolution?

28. Evolution of antibiotics resistance: 33 Billion $ annual costs in US

29. Ignoring evolution has serious health consequences

30. Evolutionary Systems Biology Group http://www.brc.hu/sysbiol/ Projects: Analyses of genetic interactions Evolution of antibiotics resistance

31. Interactions between genes are masked by distant gene duplicates Confirmed by creating corresponding triple knock-outs: Overlapping enzymatic activities between duplicates conserved across more than 100 million years of evolution