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Determination of LD50 of Phenobarbital Lab # 6
Outline Phenobarbital: MOA, SE LD 50 Determination of phenobarbital LD50
Phenobarbital Barbiturates group Antiepileptic Sedative-hypnotic
MOA Potentiates the synaptic inhibition through its action on GABA A receptors
SE Sedation Megaloblastic anemia Hypersensitivity reactions Osteomalacia In overdose, it causes coma and respiratory & circulatory failure
LD 50 The median lethal dose that kills 50% of animal population
Determination of LD50 of Phenobarbital Drugs: –Phenobarbital (2%) Instruments: –Cages. –1 ml syringes Animals: 48 mice
LD 50 Calculation Graphical method Arithmetical method (Karber’s method)
Graphical method GroupDose mg/kg Log doseDead% DeadCorrected % Probit 1330 2300 3280 4250 5220 6120 Correction formulas: For the 0% dead=100(0.25/n). For the 100% dead=100 [(n-0.25)/n] where n= number of animals in each group
Graphical method LOG DOSE Probit
Karber’s Method GroupDose (mg/kg)Dose difference (mg/kg) = a DeadMean b Product a*b 1330-8- 23003077.5225 32802056120 42503034120 5220301260 612010000.560 T= 585 - LD 50 = 330 – (585/8) =256.88 mg/kg
CNS depressants CNS depressants
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