Presentation on theme: "Drosophila as a model system Paul Adler Gilmer245 982-5475."— Presentation transcript:
Drosophila as a model system Paul Adler firstname.lastname@example.org Gilmer245 982-5475
Why is Drosophila a valuable model system? It is an animal – therefore it can be used to study development, physiology and behavior. Many genes only have functions in multicellular organism e.g. cadherins. Drosophila has been a particularly valuable model system for development. 90 years of genetics
Features shared by Drosophila and other animals and higher plants : Obligate diploid. Sexually dimorphic gametes. Pleiotropy and redundancy.
Goals for my lectures Understand Drosophila well so that you can understand a paper or seminar. Hopefully you will be comfortable enough so that you are likely to keep up with the fly literature on problems and approaches that are relevant for your research.
Drosophila has very sophisticated classical genetics and cytogenetics. These topics are often ignored these days, but they remain important in biomedical research. Because of their sophistication and power they are essential for fly genetics.
Homework Go to FlyBase and learn about cadherins in flies.
The Drosophila Genome 3 sets of autosomes –2 and 3 - large metacentric chromosome –4 - very small telocentric chromosome X/Y sex Chromosomes –X is a large telocentric chromosome
Unusual Features of Drosophila No crossing over in male meiosis larval cells (e.g. salivary gland cells) do not grow by mitotic cell division –they increase in size and become polyploid –the many chromosome strands line up to form the giant polytene chromosomes that give Drosophila it’s wonderful cytogenetics.
Polytene Chromosomes A consequence of lack of cell division in larval life (2000N). DNA strands line up in register Giant chromosomes, banding pattern (bands 5 – 200 kb). Great cytology – in favorable regions can recognize a 15 kb deletion. Uneven Amplification
Sex determination Males X/Y, 2A Females X/X, 2A Y chromosome is not male determining –X/0, 2A is a sterile males –X/X/Y, 2A is a fertile female –ratio of X to autosomes determines sex –Y chromosome is needed for male fertility
How to maintain a lethal ? Retest every generation? Balanced lethal state l 1 +/+ l 2 X l 1 +/+ l 2 If no crossing over you would get l 1 +/+ l 2, l 1 +/ l 1 + (die), + l 2 /+ l 2 (die) Problem is that crossing over generates + + chromosomes and these ruin the scheme
Mutations and Nomenclature Wild type often not stated. Semicolon between chromosomes Descriptive and humorous names. Dominants are capitalized. Allele names superscripts
y w f y w f; cn bw y w f; TM3/DcxF y w f; In(3L)fz K21 /TM6C Dr/TM3
Morphs Loss of function hypomorphs - leaky, weak amorphs - phenotypic nulls, tight, strong null - no gene product
Gain of Function Hypermorph - extra activity Neomorph - new activity antimorph - dominant negative
Mutation Nomenclature in Drosophila Loss of function: Amorphic – null m/m = m/Df Hypomorphic – some activity remains m/m < m/Df
Gain of function Hypermorphic (increased activity) m/m>m/+>m/Df Neomorphic (new activity) Antimorphic (dominant negative) m/+ >Df/+ m/+>m/Dp
Fly Resources 1. Flybase (http://flybase.bio.indiana.edu/) 2. Genome Project (http://www.fruitfly.org) 3. Allied databases (e.g. Interactive Fly – there are links for all of these on Flybase) 4. Stock Center.
Resources Sequence well annotated. Genome project cDNA clone collection. Expression patterns in embryos. Deletion collection.