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Fish Health Protocols Chris MacWilliams, DVM. Principles of Disinfection Disinfection: process that reduces or eliminates pathogenic microorganisms Cleaning.

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Presentation on theme: "Fish Health Protocols Chris MacWilliams, DVM. Principles of Disinfection Disinfection: process that reduces or eliminates pathogenic microorganisms Cleaning."— Presentation transcript:

1 Fish Health Protocols Chris MacWilliams, DVM

2 Principles of Disinfection Disinfection: process that reduces or eliminates pathogenic microorganisms Cleaning is the most important step in the cleaning and disinfection process Cleaning process has 5 steps: Dry clean Wet wash RinseDryInspect

3 Methods of Disinfection Physical –Moist heat –UV light –Drying –Exposure to sunlight Chemical –Chlorines (bleach) –Iodines (Ovadine) –Quaternary ammonium compounds (Diquat, Roccal) –Oxidizing agents (Virkon, Ozone)

4 Chemical Disinfectants Considerations: –Proven efficacy against pathogens of interest: Bacteria, virus, protozoans, fungus, spores –Safety Fish, user, equipment, environment –Affordable –Other: Presence of organic matter Effects on metals/fabrics/rubber

5 IodophorsHypochloritesOxidizersQuaternary ammonium compounds Brand NameOvadineChlorine Bleach (12%) Virkon/Peroxig ard (7%) Roccal, benzalkonium chloride Bactericidal / Virucidal /+++_ Lipophilic virucidal /++++ Sporicidal+++ ++/++_ Fungicidal++++++/++++ Effectiveness in presence of organic matter +++++/++++

6 Mechanism of action Iodophors Oxidizes proteins and interferes with metabolic reactions Hypochlorites Free hypochlorous acid attacks sulfur bonds and general structure in microbial enzymes, other proteins Oxidizers O2 release alters protein structure Quat Ammonium Cationic detergents. act against cell wall lipids in bacteria Usage notes for working solutions (not accurate for concentrate) Safe for skin contact, mild odor, color fades as potency declines, not effective if prepared with seawater Corrosive to skin and metal, strong odor, high risk to fish, volatile, not effective if prepared with seawater Safe for skin contact, mild odor/few volatiles, color fades as potency declines (Virkon), marked drop in efficacy if prepared with seawater Safe for short or occasional skin contact, odorless, some risk to fish, not effective if prepared with seawater

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8 Ovadine Egg Disinfection Why? –Vertically transmitted diseases On the surface of the egg Within an egg –Disinfecting eggs significantly decreases pathogen loads When? –During water hardening –After egg shocking and picking –After eyed eggs are transferred to a site

9 Ovadine Egg Disinfection How? 100 ppm = 10 mls of Ovadine per litre of water 100 ppm = 10 mls of Ovadine per litre of water –Determine volume of heath tray –Add appropriate amount of Ovadine to tray full of water –Gently pour rinsed, fertilized eggs into tray preloaded with Ovadine solution –After 10 minutes without disturbance, restore water flow

10 Ovadine Egg Disinfection 1 volume of eggs : 10 volumes of disinfectant solution Solution colour indicates efficacy Brown = active Brown = active Yellow = inactive and should be refreshed Yellow = inactive and should be refreshed Make a fresh Ovadine solution for each lot of eggs

11 Ovadine Egg Disinfection Spent solution disposal –Dilution is standard, combined with hatchery effluent, or can be disposed to ground Neutralize: Sodium thiosulfate 0.78 X grams of iodine x 2 (safety factor) or 0.15 gm per litre of 100ppm solution Water should be colourless before discharge to ground

12 Managing Egg Fungal Infections Egg picking –By hand –Mechanical pickers Chemical treatments –Formalin (Parasite-S TM ) –Hydrogen Peroxide –Salt –Bronophol (EDR) –Malachite Green

13 Egg Picking Dead eggs are food for fungus After shocking eyed eggs – observe eggs for mortalities

14 remove dead eggs promptly

15 Early – remove affected

16 Grape cluster – remove clumps

17 Beyond treatment – discard tray

18 Formalin Egg Disinfection 1670 ppm for 15 – 20 minutes Static bath till last few weeks till hatch –Then keep water flow >11 lpm Hanging IV bags or chicken feeders

19 Hydrogen peroxide (Perox-Aid) 500ppm for 30 – 35 minutes daily Comparatively environmentally benign Will not work on established fungal infections – only preventative

20 Others Salt – Robertson Creek every 2 – 3 days Bronophol – no benefits vs approved Malachite Green – absolutely not!

21 Sample Shipping to a Diagnostic Facility Mortalities are unexpected Clinical signs are suggestive of a disease of concern (eg. popeye and/or swollen abdomens at a facility with a history of recurrent BKD infection) Daily mortality rate exceeds 0.5% of the population

22 Selecting the samples Moribund fish preferred There may be a need to randomly sample apparently healthy fish from the population Ask Fish Path Lab staff re: sample type, numbers and shipping info

23 Before shipping Collect fish history: –population size –clinical signs –mortality and morbidity rate –diet and feed consumption –water quality conditions –records of recent stressful events (e.g. low water event, marking) –vaccination status –disease and treatment history Fill out a submission form

24 Shipping Live Fish Line a cooler with ice paks or double bagged ice Cover ice with newspaper Live fish are added to heavy duty plastic bag filled ¼ to 1/3 full of aerated ambient water Oxygen is pumped into the bag to refill it. The bag is securely closed off using elastic bands or tape. Double bag and placed in the Styrofoam cooler. Each bag is clearly marked with information identifying contents. The remaining space in the cooler is filled with cubed ice to keep the fish and water cool. The lid is placed on the Styrofoam cooler and securely fastened with duct tape to prevent accidental spillage.

25 Shipping Include a pathology laboratory submission form or an accompanying letter with more detail pathology laboratory submission formpathology laboratory submission form Include copy of mortality records if available Clearly mark sample bags The container is addressed to the laboratory at PBS and is clearly labeled with information as to originating site. Contact lab with estimated time of sample arrival

26 Shipping fresh dead fish Fresh morts (red gills, firm flesh) should be placed in labeled, sealed double plastic bags without water. Ship dead fish in a container on ice as described above for live fish. Fish should not come in contact with the ice or freezer packs.


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