Presentation on theme: "Enhancing Antibacterial Efficacy using Protein Nanoparticles Leslie Tan Zheng Yu Tan Jing Chong Erik Warnquist Varun Kulkarni Retrieved from:"— Presentation transcript:
Enhancing Antibacterial Efficacy using Protein Nanoparticles Leslie Tan Zheng Yu Tan Jing Chong Erik Warnquist Varun Kulkarni Retrieved from: http://www.eng.u ci.edu/files/images /gallery/Protein_N anoparticle_Struct ure.jpg
Introduction Pesticides are used to eradicate Agrobacterium tumefaciens High percentage of pesticide does not reach the target species. Result in water and soil pollution. Threatens biodiversity.
Introduction Usage of nanoparticles as drug carrier for pesticides Increase in therapeutic efficacy Increasing localisation to diseased sites Decrease in side effect Protein Nanoparticle are biodegradable, metabolisable and non-antigenic Does not accumulate in tissue
Objective To compare the effectiveness of antibiotic loaded albumin nanodroplets against antibiotic loaded albumin nanofibre on A. tumefaciens, grown both in vitro and in vivo.
Hypothesis The two delivery techniques will be comparable, through both qualitative and quantitative means
Variables Method of drug delivery Independent Efficacy of drug delivery system Dependent Type of bacteria (A.tumefaciens) Volume and types of antibiotic - tetracycline and ampicillin Agrobacterium volume Sizes of potato strips Temperature and humidity Controlled / constant
Materials Bovine Serum Albumin Alcohol A. tumefacians Potato strips Diffusion assays tetracycline and ampicillin
Preparation of albumin nanodroplets Emulsification Aqueous Bovine Serum Albumin is turned into an emulsion at room temperature and in oil A homogenizer is used to make the emulsion homogeneous. There is a high dispersion of particles Emulsion is added to pre-heated oil Albumin nanoparticles are separated by desolvating agent eg. Alcohol
Preparation of albumin nanofibers Electrospinning Solution inside a syringe exposed to initial electric field Electric field increases in charge Point is reached where attractive forces of charges exceeds surface tension The fibers are projected onto a grounded collector
Antibiotic loading - nanodroplets Incubating nanoparticles in antibiotic solution Antibiotic contained in nanoparticles Done at protein's isoelectric point Minimum solubility and maximum absorption BSA: pH of 4.4 Larger amount of antibiotic loaded Antibiotic entrapment efficacy measured
Antibiotic loading - nanofibres Antibiotics mixed in albumin solution Homogenous solution Hypothesis that spinning solution will result in the non polymer antibiotics also being spun
Effectiveness of antibiotic- loaded nanoparticles Protein nanoparticles digested by proteases to release antibiotics Antibiotic-loaded nanoparticles are subjected to: A.tumefacians agar plates discs A.tumefacians-potato strips
Timeline (HCI) Form droplets w/ specific concentration and temp. Load droplets with antibiotics Test droplets Send for characterization Examine results and modify original solution
Timeline (AOS) Form solution with specific concentration Spin solution Test fibers Send for characterization Examine results and modify original solution
References Buschle-Diller, G., Cooper, J., Xie, Z., Wu, Y., Waldrup, J., & Ren, X. (2007). Release of antibiotics from electrospun bicomponent fibers. Cellulose, 14(6), 553- 562 Collins, A. (2001). Agrobacterium tumefaciens. Department of Plant Pathology, University of North Carolina State. Retrieved September 19, 2010 from: http:/www.cals.ncsu.edu/course/pp728/ Agrobacterium/Alyssa_Collins_profile.htm
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