NK cell (TNFR1 -/- or TNFR2 -/-) ——Unable to respond mDCex (WT)
NK cell (WT) alone NK cell (WT) + mDCex (WT) + Isotype,DNTNF,αTNF, αTNFR1,αTNFR2 24hcell-free supernatants ELISA Fig.7 Transmembrane TNF, but not soluble TNF, mediates Dcex-induced activation of NK cells.
Similar to DCs, DCex express on their surface TNF, FasL and TRAIL, by which they can trigger caspase activation and apoptosis in tumor cells. DCex activate NK cells and stimulate them to secrete IFNγ upon the interaction of DCex TNF with NK-cell TNF receptors. DCex can mediate essential innate immune functions that were previously ascribed to DCs.