Presentation is loading. Please wait.

Presentation is loading. Please wait.

CA125: More than Just a Biomarker Manish S. Patankar, PhD University of Wisconsin-Madison Department of OB/GYN Division of Gynecologic Oncology.

Similar presentations


Presentation on theme: "CA125: More than Just a Biomarker Manish S. Patankar, PhD University of Wisconsin-Madison Department of OB/GYN Division of Gynecologic Oncology."— Presentation transcript:

1 CA125: More than Just a Biomarker Manish S. Patankar, PhD University of Wisconsin-Madison Department of OB/GYN Division of Gynecologic Oncology

2 What is CA125? Why is CA125 detected in the blood of cancer patients? Why do ovarian cancer cells produce CA125?

3 Ovarian Cancer Cell Under an Electron Microscope micrometer

4 How small are cancer cells? Diameter of a penny is millimeter or 19,050 micrometer The diameter of a cancer cell is approximately 1/700 th -1/600 th of a penny

5 Tumor Cancer cell Time Diagnosis and treatment

6 Research at University of Wisconsin-Madison Understanding the biological role of CA125 in ovarian cancer Developing immunotherapeutic agents that target ovarian cancer Developing novel therapeutic agents that can inhibit the growth of cancer cells

7 Using Natural Killer Cells (NK) to target ovarian cancer Cancer cell NK Cell Death

8 Cancer cell NK NK cells attack cancer cells

9 Boosting the anti-cancer activity of NK cells Cancer cell NK huKS-IL2 Resveratrol

10 Boosting the anti-cancer activity of NK cells Cancer cell NK huKS-IL2: Increases the ability of NK cells to bind to cancer cells Resveratrol activates NK cells Cell Death

11 Conjugated Linoleic acid (CLA) A constituent of milk and red meat CLA inhibits growth of ovarian cancer cells when tested under experimental conditions New therapeutic agents

12 Research at University of Wisconsin-Madison Understanding the biological role of CA125 in ovarian cancer Developing immunotherapeutic agents that target ovarian cancer Developing novel therapeutic agents that can inhibit the growth of cancer cells

13 What is CA125? CA125 is a tiny part of a very large molecule called MUC16 Ovarian Cancer cell CA125 MUC16

14 MUC16 Under an Electron Microscope Mol. Wt= 3-10 million Da 1-2  m in length Courtesy of Dr. John Sheehan, U. North Carolina

15 Molecular size Hemoglobin Antibody MUC16

16 Why is CA125 Detected in the Blood? Ovarian Cancer cell CA125

17 Why is CA125 Detected in the Blood? Ovarian Cancer cell CA125 Blood

18 Serum CA125 Assay CA125

19 CA125 is not useful for early detection of ovarian cancer Used only for follow-up Study from an European trial (MRC OV05/EORTC 55955) suggest that early treatment of recurrent cancer determined by rise in CA125 does not result in benefit in survival of patients

20 Ovarian Cancer cell CA125 Importance of MUC16 (CA125) in Ovarian Cancer Cell Death

21 Ovarian Cancer cell CA125 Importance of MUC16 (CA125) in Ovarian Cancer No Cell Death NK

22 Ovarian Cancer cell Importance of MUC16 (CA125) in Ovarian Cancer Cell Death NK

23 MUC16 Protects Ovarian Cancer Cells from NK cells Ovarian Cancer Ovarian Cancer NK

24 MUC16 low MUC16 medium MUC16 high NK cells selectively target csMUC16 low cells

25 MUC16 binds to NK cells NK Ovarian Cancer cell NK Blood vessel

26 Loss of MUC16 Increases Survival of Ovarian Cancer Bearing Mice MUC16 neg -OVC MUC16 pos -OVC Days Percent survival

27 Introduce agents that inhibit production of MUC16 (CA125) Ovarian Tumor Decrease in MUC16 (CA125) will Induce cancer cell death Disarm the cells and allow targeting by NK cells Introduce NK cells to target tumors

28 Our Team Dr. Arvinder Kapur, Dr. Rebecca Whelan, Mildred Felder, Kai Ludwig, Soma Banerjee, and Yang Liu Drs. David Kushner, Ellen Hartenbach, Laurel Rice, Steve Rose, Ahmed Al-Niami Drs. Nonyem Onujiogu, Mian Shahzad, Shitanshu Uppal Drs. Paul Sondel, Paul Campagnola, Pamela Kreeger, Arthur Polans, and Irwin Goldman

29


Download ppt "CA125: More than Just a Biomarker Manish S. Patankar, PhD University of Wisconsin-Madison Department of OB/GYN Division of Gynecologic Oncology."

Similar presentations


Ads by Google