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Copyright © 2009 Pearson Education, Inc. PowerPoint ® Lecture Presentation for Concepts of Genetics Ninth Edition Klug, Cummings, Spencer, Palladino Chapter.

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Presentation on theme: "Copyright © 2009 Pearson Education, Inc. PowerPoint ® Lecture Presentation for Concepts of Genetics Ninth Edition Klug, Cummings, Spencer, Palladino Chapter."— Presentation transcript:

1 Copyright © 2009 Pearson Education, Inc. PowerPoint ® Lecture Presentation for Concepts of Genetics Ninth Edition Klug, Cummings, Spencer, Palladino Chapter 5 Chromosome Mapping in Eukaryotes Lectures by David Kass with contributions from John C. Osterman. Copyright © 2009 Pearson Education, Inc.

2 Genes assort independently if they are on different chromosomes but show linkage if they are on the same chromosome. Section 5.1

3 Copyright © 2009 Pearson Education, Inc. In complete linkage, only parental (noncrossover) gametes are produced. If crossing over between two linked genes occurs between two nonsister chromatids, both parental and recombinant (crossover) gametes are produced (Figure 5.1). Section 5.1

4 Copyright © 2009 Pearson Education, Inc. Figure 5.1

5 Copyright © 2009 Pearson Education, Inc. The degree of crossing over between any two loci on a single chromosome is proportional to the distance between them, known as the interlocus distance. Section 5.1

6 Copyright © 2009 Pearson Education, Inc. If complete linkage exists between two genes because of their close proximity and organisms heterozygous at both loci are mated, a unique F 2 phenotypic ratio designated the linkage ratio results. Section 5.1

7 Copyright © 2009 Pearson Education, Inc. Genes on the same chromosome are part of a linkage group. The number of linkage groups should correspond to the haploid number of chromosomes. Section 5.1

8 Copyright © 2009 Pearson Education, Inc. The percentage of offspring resulting from recombinant gametes depends on the distance between the two genes on the chromosome. Section 5.2 Thomas H. Morgan. Alfred H. Sturtevant.

9 Copyright © 2009 Pearson Education, Inc. Synapsed chromosomes in meiosis wrap around each other to create chiasmata that are points of genetic exchange. Section 5.2

10 Copyright © 2009 Pearson Education, Inc. Two genes located relatively close to each other along a chromosome are less likely to have a chiasma form between them, and it is less likely that crossing over will occur. Section 5.2

11 Copyright © 2009 Pearson Education, Inc. The recombination frequencies between linked genes are additive, and the frequency of exchange is an estimate of the relative distance between two genes along the chromosome. Section 5.2

12 Copyright © 2009 Pearson Education, Inc. One map unit (mu) is defined as 1 percent recombination between two genes on a chromosome. Map units are often called centimorgans (cM) and are relative distances, not exact ones. Section 5.2

13 Copyright © 2009 Pearson Education, Inc. A single crossover (SCO) alters linkage between two genes only if the crossover occurs between those two genes (Figure 5.5). Section 5.2

14 Copyright © 2009 Pearson Education, Inc. Figure 5.5

15 Copyright © 2009 Pearson Education, Inc. Figure 5.6

16 Copyright © 2009 Pearson Education, Inc. Single crossovers can be used to determine the distance between two linked genes, but double crossovers (DCOs) can be used to determine the order of three genes on the chromosome and the distance between the linked genes. Section 5.3

17 Copyright © 2009 Pearson Education, Inc. To study double exchanges, three pairs of genes must be investigated, each heterozygous for two alleles (Figure 5.7). Section 5.3

18 Copyright © 2009 Pearson Education, Inc. The expected frequency of double- crossover gametes is much lower than that of either single-crossover gamete class. Section 5.3

19 Copyright © 2009 Pearson Education, Inc. In Three-Point Mapping, Parent must be heterozygous for all 3 genes under consideration. Cross constructed so genotypes can be determined by offspring phenotypes. Many offspring produced to get a representative sample of crossover events. A three-point mapping cross is shown in Figure 5.8 (Drosophila). Section 5.3

20 Copyright © 2009 Pearson Education, Inc. Figure 5.8

21 Copyright © 2009 Pearson Education, Inc. The noncrossover F 2 phenotypes occur in the greatest proportion of offspring. The double-crossover phenotypes occur in the smallest proportion. Section 5.3

22 Copyright © 2009 Pearson Education, Inc. Because the F 2 phenotypes complement each other (i.e., one is wild type and the other is mutant for all three genes), they are called reciprocal classes of phenotypes. Section 5.3

23 Copyright © 2009 Pearson Education, Inc. Distance between two genes in a three- point cross is equal to the percentage of all detectable exchanges occurring between them and includes all single and double crossovers. Section 5.3

24 Copyright © 2009 Pearson Education, Inc. Section 5.3 Determining Gene Sequence We assumed sequence is y-w-ec But don’t know if that is correct order

25 Copyright © 2009 Pearson Education, Inc. 2 methods for determining gene order from a three-point cross. Method I Method II Section 5.3

26 Copyright © 2009 Pearson Education, Inc. Section 5.3 There are only 3 possible arrangements of our 3 genes

27 Copyright © 2009 Pearson Education, Inc. Section 5.3 Method I (1) Determine arrangement of alleles on homologs of heterozygote producing crossover gametes by identifying reciprocal noncrossover phenotypes (2) test each of 3 possible orders to see which one gives you the observed double-crossover phenotypes – the one that does is the winner!

28 Copyright © 2009 Pearson Education, Inc. Section 5.3 Method II (1) Determine arrangement of alleles on homologs of heterozygote producing crossover gametes by identifying reciprocal noncrossover phenotypes (2) Look at observed double-crossover phenotypes and id single allele that has been switched Will be one that is by itself (no longer associated w/other mutant alleles) That is the one in the middle!

29 Copyright © 2009 Pearson Education, Inc. Section 5.3 Mapping Problem in Maize An example of a three-point cross and mapping of the three genes involved is shown in Figure 5.10 and Figure Linked Genes bm = brown midrib v = virescent seedling pr = purple aleurone

30 Copyright © 2009 Pearson Education, Inc. Figure 5.10

31 Copyright © 2009 Pearson Education, Inc. Figure 5.11

32 Copyright © 2009 Pearson Education, Inc. The expected frequency of multiple exchanges between two genes can be predicted from the distance between them. The coefficient of coincidence (C) is the observed number of DCOs divided by the expected number of DCOs. Section 5.4

33 Copyright © 2009 Pearson Education, Inc. Interference reduces the expected number of multiple crossovers when a crossover event in one region of the chromosome inhibits a second event nearby. Interference is positive if fewer double- crossover events than expected occur and negative if more double-crossover events than expected occur. Section 5.4

34 Copyright © 2009 Pearson Education, Inc. When two genes are close together, the accuracy of mapping is high. As the distance between them increases, the accuracy of mapping decreases. Section 5.5

35 Copyright © 2009 Pearson Education, Inc. Two exchanges between linked genes that are far apart on a chromosome can involve two, three, or all four strands. They can result in production of different percentages of recombinant chromatids (Figure 5.12). Section 5.5

36 Copyright © 2009 Pearson Education, Inc. Figure 5.12

37 Copyright © 2009 Pearson Education, Inc. Figure 5.13

38 Copyright © 2009 Pearson Education, Inc. The large numbers of mutants in Drosophila, maize, and mice have allowed extensive chromosome mapping in these organisms (Figure 5.14). Section 5.6- Drosophila Genes Have Been Extensively Mapped

39 Copyright © 2009 Pearson Education, Inc. Figure 5.14

40 Copyright © 2009 Pearson Education, Inc. Lod score analysis relies on probability calculations to demonstrate linkage between two genes in organisms in which linkage analysis relies primarily on pedigrees. Section 5.7

41 Copyright © 2009 Pearson Education, Inc. Somatic cell hybridization involves fusion of two cells in culture to form a single hybrid cell, called a heterokaryon. Section 5.7

42 Copyright © 2009 Pearson Education, Inc. Synkaryon – nuclei eventually fuse in cell Upon continued culturing of the hybrid cell, chromosomes from one of the two parental species are gradually lost until only a few chromosomes of one species remain and most chromosomes are from the other species Section 5.7

43 Copyright © 2009 Pearson Education, Inc. Figure 5.15

44 Copyright © 2009 Pearson Education, Inc. Chromosome Mapping is Now Possible Using DNA Markers and Annotated Computer Databases DNA markers represent landmarks along the chromosome. RFLPs – Restriction Fragment Length Polymorphisms Microsatellites SNPs – Single Nucleotide Polymorphisms Section 5.8

45 Copyright © 2009 Pearson Education, Inc. Recombination Occurs between Mitotic Chromosomes In Drosophila and certain fungi, homologs pair up during mitosis, allowing crossing over to take place. Sister chromatid exchanges (SCEs) occur during mitosis but do not produce new allelic combinations. Section 5.10

46 Copyright © 2009 Pearson Education, Inc. The End


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