2 Chapter 5 Outline 5.1 Linked Genes Do Not Assort Independently, 114 5.2 Linked Genes Segregate Together and Crossing Over Produces Recombination Between Them, 1165.3 A Three-Point Testcross Can Be Used To Map Three Linked Genes, 1285.4 Genes Can Be Located with Genomewide Association Studies, 137
3 The case of male baldness X-linked or not?Study of linkage of a SNP and the association with male pattern baldness shows that they do not assort independently, they are transmitted togetherPattern baldness is a hereditary trait. Recent research demonstrated that a gene for pattern baldness is linked to genetic markers located on the X chromosome, leading to the discovery that pattern baldness is influenced by variation in the androgen-receptor gene. The trait is seen in John Adams, shown here.
4 5.1 Linked Genes Do Not Assort Independently Linked genes: genes located close together on the same chromosome-Belong to the same linkage group
6 Flower color and pollen shape Figure 5.2 Nonindependent assortment of flower color and pollen shape in sweet peas.
7 F1 generation gives purple color and long pollen Appears that the purple is dominant to red and long is dominant to short pollenWhat with the F2, it is expected to get 9:3:3:1BUT…Figure 5.2 Nonindependent assortment of flower color and pollen shape in sweet peas.
8 There is no 9:3:3:1 ratio; what happened Figure 5.2 Nonindependent assortment of flower color and pollen shape in sweet peas.
9 5.2 Linked Genes Segregate Together and Crossing Over Produces Recombination Between Them Fig. 5.3Notation for crosses with linkageComplete linkage leads to nonrecombinant Gametes and nonrecombinant progenyCrossing over with linked genes leads to recombinant gametes and recombinant progenyFigs. 5.4 & 5.5
10 Figure 5.3 Crossing over takes place in meiosis and is responsible for recombination.
11 Labeling alleles of two genes on a same chromosome Cross AA BB x aa bb can be represented byA B a bA B x a bThe F1 will be:A Ba bAlso it can be represented
12 The two test cross cases Linked v.s. not linkedLinked genes give only nonrecombinant gametesUnlinked genes give both nonrecombinant and recombinantFigure 5.4 A testcross reveals the effects of linkage. Results of a testcross for two loci in tomatoes that determine leaf type and plant height.
13 Concept Check 1For single crossovers, the frequency of recombinant gametes is half the frequency of crossing over because:a. a test cross between a homozygote and heterozygote produces ½ heterozygous and ½ homozygous progeny.b. the frequency of recombination is always 50%.c. each crossover takes place between only two of the four chromatids of a homologous pair.d. crossovers take place in about 50% of meiosis.
14 What happens in recombination and the gamete formation Figure 5.5 A single crossover produces half nonrecombinant gametes and half recombinant gametes.
15 5.2 Linked Genes Segregate Together and Crossing Over Produces Recombination Between Them Calculating Recombination FrequencyRecombination frequency = (No. recombinant progeny/Total No. of progeny) 100%Fig. 5.6Coupling and Repulsion Configuration of Linked GenesCoupling (cis configuration): wild-type alleles are found on one chromosome; mutant alleles are found on the other chromosome.
16 Calculating frequencies Figure 5.6 Crossing over between linked genes produces nonrecombinant and recombinant offspring. In this testcross, genes are linked and there is some crossing over.
17 Calculating frequencies # of recombinant progenyTotal # of progenyRec. Frequency = x100%Rec. Frequency = x100%15123Rec. Frequency = x100%Figure 5.6 Crossing over between linked genes produces nonrecombinant and recombinant offspring. In this testcross, genes are linked and there is some crossing over.Rec. Frequency = 12.2 % OR
18 5.2 Linked Genes Segregate Together and Crossing Over Produces Recombination Between Them Coupling and Repulsion Configuration of Linked GenesRepulsion (trans configuration): wild-type allele and mutant allele are found on the same chromosome.Fig. 5.7Testing for Independent AssortmentFigs. 5.8 & 5.9
19 Coupling and repulsion p b+p bp bp b+Figure 5.7 The arrangement (coupling or repulsion) of linked genes on a chromosome affects the results of a testcross. Linked loci in the Australian blowfly, Lucilia cuprina, determine the color of the thorax and that of the puparium.
20 Coupling and repulsion Figure 5.8 The recombination frequency allows a prediction of the proportions of offspring expected for a cross entailing linked genes.
21 5.2 Linked Genes Segregate Together and Crossing Over Produces Recombination Between Them Gene Mapping with Recombination Frequencies:Genetic maps are determined by recombinant frequency.Map unit and centiMorgansConstructing a Genetic Map with the Use of Two-Point TestcrossesFig. 5.10
22 Figure 5.10 A two-strand double crossover between two linked genes produces only nonrecombinant gametes.
23 Recombination frequencies and distances of genes Based on recombination frequencies genetic maps are generatedActual distances are on physical mapsRecombination maps are approximations and are measured in map units or cM (centiMorgans)One map unit equals to 1% recombination frequency
24 Genetic distances measured with recombination rates are approximately additive: if the distance from gene A to gene B is 5 m.u., the distance from gene B to gene C is 10 m.u., and the distance from gene A to gene C is 15 m.u.
25 Limitations of gene mapping If the genes are far apart and the frequency is 50% we cannot say if the genes are on different chromosomes or just to far on same chromosomeTwo genes that are relatively far will give underestimate of recombination events due to possibility of multiple crosses between the genes.
26 Example: two point cross to determine map of 4 genes
27 5.3 A Three-Point Testcross Can Be Used to Map Three Linked Genes Constructing a Genetic Map with the Three-Point TestcrossFig. 5.12Determining the gene orderDetermining the location of crossoversFig. 5.13
29 A Three-Point Testcross Can Be Used to Map Three Linked Genes Figure Writing the results of a three-point testcross with the loci in the correct order allows the locations of crossovers to be determined. These results are from the testcross illustrated in Figure 7.14, with the loci shown in the correct order. The location of a crossover is indicated by a slash (/). The sex of the progeny flies has been designated arbitrarily.
30 5.3 A Three-Point Testcross Can Be Used to Map Three Linked Genes Calculating the recombination frequenciesInterference and coefficient of coincidenceEffect of multiple crossovers
31 Calculating the recombination frequencies Based on the numbers in the fruit fly testcross for three loci calculate the distances between the loci.Recombinant progeny with a chromosome that underwent crossing over between the eye-color locus (st) and the bristle locus (ss) include the single crossovers ( st+ / ss e and st / ss+ e+ ) and the two double crossovers ( st+ / ss / e+ and st / ss+ / e ); Total of 755 progeny; so the recombination frequency between ss and st is:
32 Calculating the recombination frequencies Based on the numbers in the fruit fly testcross for three loci calculate the distances between the loci.The map distance between the bristle locus (ss) and the body locus (e) is determined in the same manner. The recombinant progeny that possess a crossover between ss and e are the single crossovers st+ ss+ / e and st ss / e+ and the double crossovers st+ / ss / e+ and st / ss+ / e . The recombination frequency is:
33 Calculating the recombination frequencies Based on the numbers in the fruit fly testcross for three loci calculate the distances between the loci.Finally, calculate the map distance between the outer two loci, st and e. This map distance can be obtained by summing the map distances between st and ss and between ss and e (14.6 m.u m.u. = 26.8 m.u.). We can now use the map distances to draw a map of the three genes on the chromosome:
34 Interference and coefficient of coincidence Calculate the proportion of double-recombinant gametes by using the multiplication rule of probabilityApplying this rule, the proportion (probability) of gametes with double crossovers between st and e is equal to the probability of recombination between st and ss multiplied by the probability of recombination between ss and e, or × =Multiplying this probability by the total number of progeny gives us the expected number of double-crossover progeny from the cross: × 755 = 13.4.Only 8 double crossovers—considerably fewer than the 13 expected—were observed in the progeny of the cross
35 Interference and coefficient of coincidence Crossovers are frequently not independent events: the occurrence of one crossover tends to inhibit additional crossovers in the same region of the chromosome, and so double crossovers are less frequent than expected.The degree to which one crossover interferes with additional crossovers in the same region is termed the interference. To calculate the interference, we first determine the coefficient of coincidence, which is the ratio of observed double crossovers to expected double crossovers:
36 In our caseindicates that we are actually observing only 60% of the double crossovers that we expected on the basis of the single-crossover frequencies.Calculation of interferenceInterference = 1 − coefficient of coincidenceSo the interference for our three-point cross is:interference = 1 − 0.6 = 0.440% of recombinations are not observed due to intefrerence
37 5.4 Genes Can Be Located with Genomewide Association Studies Linkage analysis (genetic markers; anonymous markers)Genome wide association studiesHaplotypeLinkage disequilibrium
38 Figure 5.17 Genomewide association studies are based on the nonrandom association of a mutation (D–) that produces atrait and closely linked genes that constitute the haplotype.
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