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Lab 11& 12 Biochemical Characterization Practice questions Designed by Pyeongsug Kim ©2010 Fall 2011 Chattahoochee Technical College Picture from
Designed by Pyeongsug Kim, ©2010 What is the name of each medium? Identify whether each agar is selective or differential. Picture from D MSA agar – both selective and differential TSA agar – Neither Blood agar – Differential EMB agar – both selective and differential A: B: C: D:
Designed by Pyeongsug Kim, ©2010 What is the name of the media above? Is selective or differential media? Explain your answer. Is it shown positive result of negative result in each medium? A: B: C: Picture from A B C EMB agar Both selective and differential Selective ~inhibit the growth of Gram positive bacteria (selective for Gram negative) Differential ~ differentiates coliforms (Lactose fermenters) Positive (lactose fermentation, coliforms) Positive (Lactose fermentation, coliforms) Negative What is the name of the media above? Is selective or differential media? Explain your answer.
Designed by Pyeongsug Kim, ©2010 AA Is it shown positive result of negative result in each medium? A: B: What is the name of the media above? Is selective or differential media? Explain your answer. MSA plate Both selective and differential Selective ~Selective for Gram positive and Salt tolerant Differential ~ differentiates for mannitol fermentation Positive (manitol fermentation) Negative Picture from
What results are shown? A: B: C: Picture from Designed by Pyeongsug Kim, ©2010 What is the name of the test above? What is the name of media above? Is selective or differential media? Explain your answer. Hemolysys test Differential ~ differentiates for hemolysis Alpha hemolysis Gamma hemolysis Beta hemolysis Blood agar AB C B
Designed by Pyeongsug Kim, ©2010 What properties of bacterial metabolism are demonstrated above? A: B: C: Is selective or differential media? Explain your answer. Sugar fermentation Differential ~ differentiates for sugar fermentation Positive ~ Lactose fermentation (acid/ No gas) Positive ~ Dextrose fermentation (acid/gas forming) Negative for maltose fermentation (No acid/ No gas) Phenol red broth with sugar Each tube contains …. A: Lactose B: Dextrose C: Maltose. A B C Picture from What is the name of media?
Semisolid media What properties of bacterial metabolism are demonstrated above? A: B: A B S Positive for Hydrogen Sulfide production S Negative for Hydrogen Sulfide production Designed by Pyeongsug Kim, ©2010 What is the name of media? Is it selective or differential? SIM -Differential A B What name of the media is shown? Is it selective or differential? SIM; Differential What properties of bacteria is shown? A: B: Motility Nonmotile Motile Picture from
Designed by Pyeongsug Kim, ©2010 Kovac’s reagent What is the name of media? Is it selective or differential? SIM; Differential What name of the test is? Indole test AB Control What biological prosperity do this bacteria have? A: B: Negative for indole production Positive for indole production Picture from
What is the name of media? Is it selective or differential? Designed by Pyeongsug Kim, ©2010 Methyl red Picture from What is the name of the test(left)? MR-VP; Differential What biological prosperity do this bacteria have? A: B: A B Negative for Mixed acid production Positive for Mixed acid production VP test What is the name of the test(left)? What biological prosperity do this bacteria have? A: B: MR test Negative for acetoin production Positive for acetoin production
Designed by Pyeongsug Kim, ©2010 Picture from: A B Before putting the reagent. After putting the reagent. What is the name of the test(left) using a starch agar? What biological prosperity do this bacteria have? A: B: Extracellular amylase test Negative-No amylase presence Positive - Amylase presence What is the name of the reagent that used in this test? Gram-Iodine Which one, A or B, is shown starch presence? A
Designed by Pyeongsug Kim, ©2010 What is the name of the test(left)? Hydrogen peroxide Picture from Catalase test What type of reagent is using for this test? What biological prosperity do this bacteria have? A: B: Negative – Catalase presence. Positive – Catalase presence
Biochemical Characterization Supplemental instruction Designed by Pyeongsug Kim ©2010 Fall 2011 Chattahoochee.
Summary of Biochemical Tests in Microbiology Better living through chemistry.
Media!. Figure 1.16 Bacterial colonies on agar Bacterium 1 Bacterium 2 Bacterium 3 Bacterium 4 Bacterium 5 Bacterium 6 Bacterium 7 Bacterium 8 Bacterium.
General Microbiology Laboratory Biochemical tests.
Selected and Differential Media. Define Selective Media Differential Media Enriched Media.
Lab #8. Review of Lab #7 - pH Indicators pH Indicator Very acidic AcidicNeutralBasic Phenol red- pH <6.8 = yellow pH = red pH >8.0 = magenta/
Media & Biochemical Tests Laboratory Objectives. Tests To Know Case Study Tests Indole Methyl Red/Voges Proskauer Citrate H 2 S production in SIM Urea.
Gram Positive Staphylococci – (Note the purple, spherically- shaped, clustered cells)
EMB agar Only gram-negative bacteria grow on EMB agar. (Gram-positive bacteria are inhibited by the dyes eosin and methylene blue added to the agar.) Based.
Biochemical Activities of Microorganisms Part (2).
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Lab #9. Review - pH Indicators pH Indicator Very acidic AcidicNeutralBasic Phenol red- pH <6.8 = yellow pH = red pH >8.0 = magenta/ hot pink.
Week 8 W Exp 22: Carbohydrate Fermentation Lab Two pg 158 Exp 23: Triple Sugar Iron Agar Test Lab Two pg 162 Charts New: Exp 24: IMViC Part A: Indole Test.
SC246 Microbiology. Lab: Unknown Identification Microorganisms are the most common and the most numerous group of organisms on Earth. The world of Microbiology.
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Lab 10 BACTERIOLOGY OF THE GASTROINTESTINAL TRACT Lab Manual (p.67-82)
CHAPTER 3. Content purification of cultures morphological and pureculture studies biochemical tests.
- It is nutritive ingredient needed by a particular m.o to enhance it growth under laboratory condition. -Different type of media: 1- Growth media(general.
Introduction to Lab Ex. 16 Diagnostic Media Urea broth Sulfide Indole Motility Citrate.
Bacteria identification : Media. What you have to know about the media What are the sources of C,H,N,O,P,S? What type of media is it? What are the indicators?
SELECTIVE, DIFFERENTIAL AND ENRICHED MEDIA. Selective media: used to select (isolate) specific groups of bacteria; chemical substances in the media inhibit.
Physiological characteristics: Oxidative and fermentation tests Lab # 8 Medgar Evers College Prof. Santos.
Lab. No. 4 (A). StaphylococciStreptococciMicrococci NeisseriaCorynbacterium Clostridum Bacillus Enterobacteriaceae Pseudomonas. Bacteria Gram’s Stain.
Lab Exercise: 15 Enzymes: 1.Catalase 2.Proteinase 3.MR-VP.
Identifying Bacteria based on Enzymes and multiple test media Lab # 9 Medgar Evers College Prof. Victor Santos.
General Microbiology Laboratory Selective and Differential media.
Culture Media (Types, Preparation & Sterilization)
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Lab 13- Bacterial cultivation Selective /differential media.
General Microbiology Laboratory Media Preparation & Sterilization.
WEEK 8 Identification of Staphylococci and Streptococci.
Differential medium. Differential medium helps us to differentiate one group of bacteria from another. Blood agar – differentiate hemolytic bacteria from.
Lab Exercise 17: Biochemical Differentiation of some Medically Important Gram-negative Bacilli.
Lab 14 Goals and Objectives: Exercise 69: Staphylococci Identification Read and record results Exercise 70: Streptococci & Enterococci Identification Read.
Lab 11 Goals and Objectives: Exercise 39: Oxidation and Fermentation Tests Read results: some tubes will require additional reagents (share reagents across.
Mic 224 Lab 10 IMViCs. IMViC Tests The IMViC tests are useful for differentiating the Enterobacteriaceae, especially when used alongside the urease test.
PHT 416 Lab 8. Steps Microscopic Morphology Growth Biochemical Tests Nutrient agar Blood agar Mannitol Salt Agar MacConkey’s agar.
Types of Agar. MacConKey Agar designed to grow Gram-negative bacteria and stain them for lactose fermentation. contains bile salts (to inhibit most Gram-positive.
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Ex. 18: IMViC Reactions Objectives ?? Indole production test Methyl red test Voges-Proskauer test Citrate utilization test.
Media Preparation and Sterilization. A medium is sterilized (living organisms removed) before usage in the lab. Sterilization methods include; autoclaving,
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