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Lab 11& 12 Biochemical Characterization Practice questions Designed by Pyeongsug Kim ©2010 Fall 2011 Chattahoochee.

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Presentation on theme: "Lab 11& 12 Biochemical Characterization Practice questions Designed by Pyeongsug Kim ©2010 Fall 2011 Chattahoochee."— Presentation transcript:

1 Lab 11& 12 Biochemical Characterization Practice questions Designed by Pyeongsug Kim ©2010 Fall 2011 Chattahoochee Technical College Picture from

2 Designed by Pyeongsug Kim, ©2010 What is the name of each medium? Identify whether each agar is selective or differential. Picture from D MSA agar – both selective and differential TSA agar – Neither Blood agar – Differential EMB agar – both selective and differential A: B: C: D:

3 Designed by Pyeongsug Kim, ©2010 What is the name of the media above? Is selective or differential media? Explain your answer. Is it shown positive result of negative result in each medium? A: B: C: Picture from A B C EMB agar Both selective and differential Selective ~inhibit the growth of Gram positive bacteria (selective for Gram negative) Differential ~ differentiates coliforms (Lactose fermenters) Positive (lactose fermentation, coliforms) Positive (Lactose fermentation, coliforms) Negative What is the name of the media above? Is selective or differential media? Explain your answer.

4 Designed by Pyeongsug Kim, ©2010 AA Is it shown positive result of negative result in each medium? A: B: What is the name of the media above? Is selective or differential media? Explain your answer. MSA plate Both selective and differential Selective ~Selective for Gram positive and Salt tolerant Differential ~ differentiates for mannitol fermentation Positive (manitol fermentation) Negative Picture from

5 What results are shown? A: B: C: Picture from Designed by Pyeongsug Kim, ©2010 What is the name of the test above? What is the name of media above? Is selective or differential media? Explain your answer. Hemolysys test Differential ~ differentiates for hemolysis Alpha hemolysis Gamma hemolysis Beta hemolysis Blood agar AB C B

6 Designed by Pyeongsug Kim, ©2010 What properties of bacterial metabolism are demonstrated above? A: B: C: Is selective or differential media? Explain your answer. Sugar fermentation Differential ~ differentiates for sugar fermentation Positive ~ Lactose fermentation (acid/ No gas) Positive ~ Dextrose fermentation (acid/gas forming) Negative for maltose fermentation (No acid/ No gas) Phenol red broth with sugar Each tube contains …. A: Lactose B: Dextrose C: Maltose. A B C Picture from What is the name of media?

7 Semisolid media What properties of bacterial metabolism are demonstrated above? A: B: A B S Positive for Hydrogen Sulfide production S Negative for Hydrogen Sulfide production Designed by Pyeongsug Kim, ©2010 What is the name of media? Is it selective or differential? SIM -Differential A B What name of the media is shown? Is it selective or differential? SIM; Differential What properties of bacteria is shown? A: B: Motility Nonmotile Motile Picture from

8 Designed by Pyeongsug Kim, ©2010 Kovac’s reagent What is the name of media? Is it selective or differential? SIM; Differential What name of the test is? Indole test AB Control What biological prosperity do this bacteria have? A: B: Negative for indole production Positive for indole production Picture from

9 What is the name of media? Is it selective or differential? Designed by Pyeongsug Kim, ©2010 Methyl red Picture from What is the name of the test(left)? MR-VP; Differential What biological prosperity do this bacteria have? A: B: A B Negative for Mixed acid production Positive for Mixed acid production VP test What is the name of the test(left)? What biological prosperity do this bacteria have? A: B: MR test Negative for acetoin production Positive for acetoin production

10 Designed by Pyeongsug Kim, ©2010 Picture from: A B Before putting the reagent. After putting the reagent. What is the name of the test(left) using a starch agar? What biological prosperity do this bacteria have? A: B: Extracellular amylase test Negative-No amylase presence Positive - Amylase presence What is the name of the reagent that used in this test? Gram-Iodine Which one, A or B, is shown starch presence? A

11 Designed by Pyeongsug Kim, ©2010 What is the name of the test(left)? Hydrogen peroxide Picture from Catalase test What type of reagent is using for this test? What biological prosperity do this bacteria have? A: B: Negative – Catalase presence. Positive – Catalase presence


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