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Biotechnology Unit 1 : Dairy Industries i. Milk ii. Yoghurt iii. Cheese iv. Environmental Impact.

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Presentation on theme: "Biotechnology Unit 1 : Dairy Industries i. Milk ii. Yoghurt iii. Cheese iv. Environmental Impact."— Presentation transcript:

1 Biotechnology Unit 1 : Dairy Industries i. Milk ii. Yoghurt iii. Cheese iv. Environmental Impact

2 i. Milk Milk is an important food for most British people.Milk is an important food for most British people. It is an important part of a balanced dietIt is an important part of a balanced diet Milk contains:Milk contains:fatstarchsugarproteinminerals

3 Milk treatment Milk treatment All milk comes from dairy cows. It is treated in different ways to produce different types. Milk is available in forms such as: pasteurised skimmed UHT (ultra high temperature) powder

4 Heat treatment of milk Milk is an ideal place for bacteria to grow.Milk is an ideal place for bacteria to grow. Some bacteria are harmful so all milk is heat treated to kill them.Some bacteria are harmful so all milk is heat treated to kill them. Common methods of heat treating are by:Common methods of heat treating are by:pasteurisation Ultra High Temperature

5 Pasteurisation Most milk is treated by pasteurisation. Method: 1. Heat milk to 72ºC for 15 seconds. 2. Cool quickly to below 10ºC. 3. Pack in bottle, carton or container. Pasteurised milk will keep for up to five days in a fridge.

6 Ultra High Temperature UHT milk is heated to a higher temperature than pasteurised milk. UHT milk is heated to 135 o C- 142 o C for 2-5 seconds. This process alters the taste of milk.

7 Resazurin Test Resazurin dye is a chemical that changes colour in response to the number of bacteria in a liquid.Resazurin dye is a chemical that changes colour in response to the number of bacteria in a liquid. Can be used to tell us if milk is fit to drink.Can be used to tell us if milk is fit to drink.

8 Colour of sample Bacterial content Drinking quality of milk Blue-purple Very low Good mauvelowSatisfactory pinkmediumPoor whitehighunsatisfactory

9 Experiment: resazurin test Fresh milk + resazurin 5 day old milk + resazurin 10 day old milk + resazurin

10 Results: Time (min) Fresh milk 5 day- old milk 10 day- old milk

11 Fat content of milk Type of milk Fat removed Whole milk none Semi-skimmedhalf skimmed almost all Milk can be graded by its fat content: Evaporated milk has ½ the water removed and is used like cream.

12 Content of milk (continued) Removing fat from milk reduces vitamin content e.g. Vitamin ARemoving fat from milk reduces vitamin content e.g. Vitamin A Skimmed and semi-skimmed milk have fat removed so the vitamin content is reduced.Skimmed and semi-skimmed milk have fat removed so the vitamin content is reduced. Young children should be given whole milk which has more vitamins.Young children should be given whole milk which has more vitamins.

13 ii. Yoghurt Milk can be preserved (made to last longer) by changing it into yoghurt or cheese.Milk can be preserved (made to last longer) by changing it into yoghurt or cheese. Natural yoghurt can be used as ‘starter cultures’ to make yoghurt in the lab.Natural yoghurt can be used as ‘starter cultures’ to make yoghurt in the lab.

14 Making Yoghurt Starter cultures contain special bacteria that make lactic acid from the sugar (lactose) in the milk.Starter cultures contain special bacteria that make lactic acid from the sugar (lactose) in the milk. lactoselactic acid lactoselactic acid (sugar in milk)(thickens and gives taste) (sugar in milk)(thickens and gives taste) Lactic acid thickens the milk and gives the yoghurt its taste.Lactic acid thickens the milk and gives the yoghurt its taste.

15 Making yoghurt Method 1.Heat milk to 43 o C (helps bacteria grow) 2.Add 1 teaspoon of starter culture (natural yoghurt) 3.Cover with cling film. 4.Incubate yoghurt at 43 o C for 7 hours. 5.When ready, place yoghurt in fridge for 4 hours.

16 Types of Yoghurt There are different methods for making yoghurts: 1. Stirred yoghurts – bacteria is added to the batch. It is then put in to pots when ready. 2. Set yoghurts – bacteria is added then the mixture is put straight into the pots where it sets.

17 Aseptic conditions Air contains many types of microbe.Air contains many types of microbe. Many are also present in dust as tiny clumps called spores.Many are also present in dust as tiny clumps called spores. During experiments, certain precautions should be taken to create sterile (aseptic) conditions.During experiments, certain precautions should be taken to create sterile (aseptic) conditions.

18 This is done for two reasons:This is done for two reasons: –To stop unwanted microbes getting into the experiment and spoiling it –To stop ant microbes used in the experiment escaping.

19 1. Hands should be washed, cuts should be covered.1. Hands should be washed, cuts should be covered. 2. Work surfaces should be disenfected.2. Work surfaces should be disenfected. 3. Lab coats should be worn3. Lab coats should be worn 4. All equipment should be sterilised in an autoclave (heated to 121C for 20 mins).4. All equipment should be sterilised in an autoclave (heated to 121C for 20 mins).

20 iii. Cheese Making Cheese Milk is pasteurised to kill most bacteria.Milk is pasteurised to kill most bacteria. Special bacteria are added to convert milk sugar (lactose) into lactic acid.Special bacteria are added to convert milk sugar (lactose) into lactic acid. Enzymes (rennet) are added to clot the proteins in milk to form solid cheese.Enzymes (rennet) are added to clot the proteins in milk to form solid cheese.

21 Types of rennet Type of rennet SourceAdvantageDisadvantage Calf rennet calves Original source, used for centuries. Animals must be killed, risk of disease Fungal rennet fungus Cheap, large amounts, OK for vegetarians. taste GM yeast rennet yeast No animals involved, OK for vegetarian, same as animal rennet. Public concern about genetically modified foods Milk clotting enzymes (rennet) can come from different sources.

22 iv.Environmental Impact: Monitoring Waste Cheese making uses the enzyme rennet which makes the milk proteins clot to form curd.Cheese making uses the enzyme rennet which makes the milk proteins clot to form curd. The liquid left is called whey.The liquid left is called whey. milk + rennet curds (solid) whey (liquid) cheese waste product

23 Whey and pollution Whey and pollution Whey contains sugar. What would happen if whey was released into rivers?Whey contains sugar. What would happen if whey was released into rivers? 1. Bacteria would use the whey sugars as food and reproduce. 2. As the number of bacteria increased it would use up the oxygen so oxygen levels would decrease in the water. 3. Fish and other living organisms would start to die as the oxygen level decreased.

24 Pollution prevention To prevent pollution whey can be:- a. treated before release b. upgraded (used for something else)

25 Treatment of whey Add bacteria which feed on whey and turn it into carbon dioxide and water.Add bacteria which feed on whey and turn it into carbon dioxide and water. Remove bacteria and release cleaner water into river.Remove bacteria and release cleaner water into river. Test water oxygen level to make sure it is OK.Test water oxygen level to make sure it is OK.

26 Upgrading whey Waste whey used as food for growing some types of yeast.Waste whey used as food for growing some types of yeast. In the right conditions these yeast strains produce alcohol from the sugars in the whey.In the right conditions these yeast strains produce alcohol from the sugars in the whey. Alcohol produced is creamy (found in Baileys Irish Cream)Alcohol produced is creamy (found in Baileys Irish Cream)

27 Preventing pollution

28 Biotechnology Yeast Industries Yeast Industries i. Bread ii. Beer iii. Fermented milk drinks iv. Flavouring and food colouring v.Environmental impact

29 i. Bread Yeast: a single-cell fungus (plant)a single-cell fungus (plant) used in bread-making for 1000’s of yearsused in bread-making for 1000’s of years is added to flour to make bread rise (dried or fresh yeast – activity 2.1)is added to flour to make bread rise (dried or fresh yeast – activity 2.1)

30 Yeast (continued) Yeast is a living organism.Yeast is a living organism. It respires to release carbon dioxide.It respires to release carbon dioxide. It is the carbon dioxide that makes bread rise.It is the carbon dioxide that makes bread rise.

31 Growing yeast Huge numbers of pure yeast can be grown in large vessels called fermenters.Huge numbers of pure yeast can be grown in large vessels called fermenters. This yeast can be used in the baking or brewing industry to make bread or beer.This yeast can be used in the baking or brewing industry to make bread or beer. Cultures of pure yeast can be grown on an agar plate.Cultures of pure yeast can be grown on an agar plate.

32 ii. Beer Beer is an alcoholic drink made from: waterbarleysugarhopsyeast

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34 The role of yeast in making beer sugar alcohol + carbon dioxide + energy + energy Yeast uses sugar to release energy. During this process, called fermentation, alcohol and carbon dioxide are released. The gas carbon dioxide is what makes the beer fizzy. yeast

35 Ales and lagers Different strains of yeast give different ales and lagers.Different strains of yeast give different ales and lagers. These yeasts use the sugars at different rates and at different temperatures.These yeasts use the sugars at different rates and at different temperatures.

36 Ale and lager yeasts Type of yeast Growth temp Time to grow Position of yeast in vessel Ale12-18ºC 6 days Rise to top Lager8-12ºC 21 days Sink to bottom

37 Making beer There are over 1200 different brands of beer in Britain each with its own flavour.There are over 1200 different brands of beer in Britain each with its own flavour. Around half the beer is lager, the rest is bitter, ale and stout.Around half the beer is lager, the rest is bitter, ale and stout. Different beers are brewed in different ways and have different alcohol contents.Different beers are brewed in different ways and have different alcohol contents. Traditional beers have around 4% alcoholTraditional beers have around 4% alcohol (activity 2.3)

38 Alcohol contents of beer (Activity 2.4) Drink Alcohol Content Lager Barley ale Low alcohol beer Alcohol free beer Wine Vodka Whiskey

39 Alcohol contents of beer (Activity 2.4) Drink Alcohol Content Lager 4% 4% Barley ale 11% 11% Low alcohol beer 1% 1% Alcohol free beer 0.05% 0.05% Wine 12% 12% Vodka 40% 40% Whiskey

40 Maturing the beer Beer must be matured before it can be drunk. Maturing beer: –Improves flavour –Removes any solids –Gives ‘sparkle’ (activity 2.6) (activity 2.6)

41 Beer can be: –cask conditioned (often called real ale) –brewery conditioned (kegs, bottle, cans)

42 Cask conditioned beer Cask conditioned beer is put into casks (huge containers madeCask conditioned beer is put into casks (huge containers made from wood or steel) Sugar is added to the cask.Sugar is added to the cask. Yeast still producing carbon dioxide which makes the beer ‘sparkle’.Yeast still producing carbon dioxide which makes the beer ‘sparkle’. Beer produced is dark with a strong flavour.Beer produced is dark with a strong flavour.

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44 Brewery conditioned beer Stored in large tanksStored in large tanks Sold in kegs,Sold in kegs, bottles or cans. Remains of yeast and other solids removed.Remains of yeast and other solids removed. Beer (e.g.) lager is clear and bright.Beer (e.g.) lager is clear and bright. Long shelf life (keeps for a long time)Long shelf life (keeps for a long time)

45 Differences : cask conditioned and brewery conditioned beers (Activity 2.6) Cask conditioned beer Brewery conditioned beer Example of type of beer produced Storage conditions Description of beer

46 Differences : cask conditioned and brewery conditioned beers (Activity 2.6) Cask conditioned beer Brewery conditioned beer Example of type of beer produced Real ale e.g. Lager Keg beers Bottles/cans Storage conditions Casks Sugar added Yeast present Large tanks Yeast removed Description of beer Dark colour High flavour Clear/bright Lasts longer

47 iii. Fermented Milk Drinks In many countries it is difficult to keep milk and yoghurt fresh. The milk can be fermented slightly to make it alcoholic. Yeast is used to turn the sugars in milk into alcohol.

48 Making fermented milk drinks (activity 2.7/2.8) Kefir is a refreshing, fizzy, slightly alcoholic, yoghurt drink.Kefir is a refreshing, fizzy, slightly alcoholic, yoghurt drink. This drink can be made by a method called immobilisation.This drink can be made by a method called immobilisation.

49 Making Kefir Step 1 Sodium alginate + lactase (enzyme that breaks down lactose) Sodium alginate + lactase (enzyme that breaks down lactose) Add wine yeast Add wine yeast Add calcium chloride drop by drop drop by drop (hardens beads) (hardens beads) Immobilised beads

50 Step 2 Warm milk Add live yoghurt Add live yoghurt Add Immobilised beads Leave at 43ºC for 5 hours Filter mixture to give Filter mixture to give fermented milk drink fermented milk drink Beads can be re-used kefir

51 Immobilisation Immobilisation can be used to trap an enzyme and some yeast into a jelly bead. Advantages: 1.After the reaction the beads can be washed and re-used. 2.Saves money (enzymes are expensive) 3.Bead easily separated from product (e.g. by filtering) jelly coat yeast + enzyme

52 iv. Food flavouring Yeast can be used for:Yeast can be used for: –Making bread –Alcoholic drinks –Flavouring food Foods with yeast flavouring:Foods with yeast flavouring: –Meat flavoured crisps e.g. chicken,bacon –Oxo cubes –Marmite (activity 2.9)

53 Food colourings (activity 2.10) Wild salmon and trout have pink flesh.Wild salmon and trout have pink flesh. This colour comes from the pink coloured prawns and shrimps they eat. Farmed salmon would have grey flesh but they are fed red dye just before they are killed which makes their flesh pink.Farmed salmon would have grey flesh but they are fed red dye just before they are killed which makes their flesh pink. Red dye

54 Feeding dye doesn’t affect theFeeding dye doesn’t affect the flavour but makes their flesh more appealing to eat. Now red yeast can be fed to the fish. This gives a pink colour to their flesh.Now red yeast can be fed to the fish. This gives a pink colour to their flesh. Red yeast

55 v. Environmental Impact Waste from yeast industries should not be dumped in rivers.Waste from yeast industries should not be dumped in rivers. Yeast would act as food for bacteria which would cause pollution.Yeast would act as food for bacteria which would cause pollution.

56 Getting rid of waste Waste can beWaste can be upgraded and upgraded and used for animal used for animalfeed. Treated water shouldTreated water should be tested before releasing into rivers.

57 The methylene blue test We are going to test some water samples to see if they could be put into a river. Collect: samples A, B and C Collect: samples A, B and C 3specimen tubes+lids 3specimen tubes+lids 3 labels 3 labels dropper dropper measuring cylinder measuring cylinder methylene blue dye methylene blue dye

58 Testing water samples for pollution: The Methylene Blue Test Time Colour change Water condition Pollution scale Immediate 2-3 days 4-5 days

59 The methylene blue test Time Colour change Water condition Pollution scale Immediate Blue to clear Dangerou s for river Very polluted 2-3 days Blue to clear Dangerou s for river Very polluted 4-5 days Blue to clear Needs more treatment Slightly polluted 4-5 days Still blue Safe for release Not polluted

60 Detergent Industries Detergent Industries

61 Enzymes in washing powders The word detergent means ‘something that cleans’ e.g. soaps soaps washing up liquid washing powder

62 Biological washing powders Biological washing powders contain enzymes. Enzymes are chemicals that improve the way in which the powder cleans.

63 What is biological washing powder made of? Biological washing powder is made up of: 1% enzymes1% enzymes 99% water softeners99% water softenersbleach other chemicals (to help water get into the clothes)

64 Where do the enzymes in washing powder come from? Bacteria are tiny organisms found almost everywhere on Earth.Bacteria are tiny organisms found almost everywhere on Earth. Scientists found bacteria that were harmless and produced enzymes that could be used in washing powders.Scientists found bacteria that were harmless and produced enzymes that could be used in washing powders. Large numbers of these bacteria grow (cultured) very quickly in huge industrial fermenters that give the best conditions for growth.Large numbers of these bacteria grow (cultured) very quickly in huge industrial fermenters that give the best conditions for growth. Enzymes produced are then separated from the bacteria and used to make biological washing powder.Enzymes produced are then separated from the bacteria and used to make biological washing powder.

65 The use of enzymes in washing powders Enzymes in washing powders digest the stains on clothes like enzymes in the gut digest food. Different enzymes digest different stains. –Fat digesting enzymes digest fatty stains. –Starch digesting enzymes digest fatty stains. Enzymes make up a small part of powder but a large part of the cleaning power!

66 Activity 3.1: To show how an enzyme can remove a stain Stains from food like eggs contain protein.Stains from food like eggs contain protein. Photographic film has protein on its surface.Photographic film has protein on its surface. In the following experiment, enzymes like those in biological washing powder are used to remove the ‘stain’ on a piece of photographic film Protein ‘stain’

67 Expt. : To investigate the effect of enzymes on a protein stain Method: Collect 2 test tubes.Collect 2 test tubes. Label test tubes A and BLabel test tubes A and B Add 1 piece of film to each test tube.Add 1 piece of film to each test tube. Half fill tube A with enzymeHalf fill tube A with enzyme Half fill tube B with waterHalf fill tube B with water Shake each tubeShake each tube Put tubes in water bath at 50 o C for 30 mins – shake tubes every 5 minsPut tubes in water bath at 50 o C for 30 mins – shake tubes every 5 mins Remove film, dry and examine.Remove film, dry and examine.

68 Tube contents Protein digested? Film + Enzyme Film + water

69 Comparing biological and non- biological washing powders Biological washing powders contain enzymes.Biological washing powders contain enzymes. Non-biological washing powders do not contain enzymes.Non-biological washing powders do not contain enzymes. This experiment compares the ability of these two types of washing powder to remove different stainsThis experiment compares the ability of these two types of washing powder to remove different stains

70 Comparing bio and non- bio washing powders Stained cloth Warm water + bio powder Warm water +non- bio powder

71 Stain Removed with bio powder? Removed with non-bio powder?

72 Disadvantage of biological washing powders Original biological washing powders sometimes caused an allergic reaction in some people.Original biological washing powders sometimes caused an allergic reaction in some people. This caused skin rashes, eczema and asthma.This caused skin rashes, eczema and asthma. New powders are now produced with enzymes enclosed in a harmless waxy coating.New powders are now produced with enzymes enclosed in a harmless waxy coating. This helps to prevent allergic reactions.This helps to prevent allergic reactions. (Activity 3.3)

73 Advantages of biological washing powders (Activity 3.4) 1.Adding enzymes to biological washing powders means cleaner clothes (stains are digested). 2. Work best at low temperatures 40 o C - 55 o C (temperatures above 60 o C destroy enzymes) so need to heat clothes to high temperatures to get them clean-saves energy and money. 3. Lower temperature used with biological washing powders reduce damage to delicate fabrics.

74 Environmental Impact : Monitoring Waste Making detergent uses energy for: ProductionProduction PackagingPackaging TransportingTransporting But most energy is used in the home for: The wash cycleThe wash cycle Tumble dryingTumble drying IroningIroning

75 Detergents and energy Activity 3.5 To provide all the energy needed power stations burn coal, oil or gas. This gives off carbon dioxide and sulphur dioxide that and sulphur dioxide that pollute the atmosphere. Low temperature wash = less energy good for public and environment!!

76 One manufacturer of detergents has set targets for waste management: One manufacturer of detergents has set targets for waste management: Reduce weight and volume of packingReduce weight and volume of packing Use reusable materialsUse reusable materials Encourage recycling programmesEncourage recycling programmes Encourage safe disposal practisesEncourage safe disposal practises New ‘Micro’ powders and liquids use less packaging and powder for each wash.

77 Detergents and the Environment (Activity 3.6) Detergents are flushed away as waste water and can pollute the environment.Detergents are flushed away as waste water and can pollute the environment. Detergents can be toxicDetergents can be toxic (poisonous) to wildlife. Manufacturers test products to check they won’t harm fish or other living organisms in rivers.Manufacturers test products to check they won’t harm fish or other living organisms in rivers.

78 Detergents containing phosphates and sulphates pollute river Tiny plants (algae) reproduce quickly to form ‘bloom’ Algae die Bacteria feed on dead algae Number of bacteria increases Bacteria use up oxygen in water Fish and animals die

79 Reducing Environmental Impact Detergents in water tested at sewage works in mini sewage plantsDetergents in water tested at sewage works in mini sewage plants Sewage plants could remove the phosphates and sulphates to reduce environmental impactSewage plants could remove the phosphates and sulphates to reduce environmental impact Using low phosphate detergents can also reduce environmental impactUsing low phosphate detergents can also reduce environmental impact

80 Washing clothes in other countries (Activity 3.7) Most people in the worldMost people in the world wash clothes by hand Washing machines in other countries are different from those in the UKWashing machines in other countries are different from those in the UK  In the USA washing machines are bigger and use more water  In Japan and Taiwan washing machines do not heat the water. Clothes are pre- soaked and washed more often

81 Biotechnology Unit 4 : Pharmaceutical Industries i. Antibiotics ii. Antifungals

82 Antibiotics ~ discovered in London in 1928 by Alexander Fleming (Scottish scientist) ~ He was growing bacteria on agar plates to study. ~ He was growing bacteria on agar plates to study. ~ One of his plates had been contaminated with a fungal spore and he noticed no bacteria would grow near it ~ One of his plates had been contaminated with a fungal spore and he noticed no bacteria would grow near it ~He found out that fungi produce chemicals to stop growth of competing bacteria ~He found out that fungi produce chemicals to stop growth of competing bacteria

83 ~These chemicals are called antibiotics ~These chemicals are called antibiotics ~The antibiotic Fleming had discovered was penicillin and had been produced by the fungi penicillium ~The antibiotic Fleming had discovered was penicillin and had been produced by the fungi penicillium ~Different types of antibiotics kill different bacteria – but not viruses like those causing flu or the cold. ~Different types of antibiotics kill different bacteria – but not viruses like those causing flu or the cold.

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85 Other scientists developed ways of extracting peniciliin fron the fungus and purifying itOther scientists developed ways of extracting peniciliin fron the fungus and purifying it The first man to be treated with penicillin was a policeman.The first man to be treated with penicillin was a policeman. He was ill from blood poisoning and not expected to live more than a few hours.He was ill from blood poisoning and not expected to live more than a few hours. When injected with penicillin there was a huge improvement in his condition.When injected with penicillin there was a huge improvement in his condition. The man only died when the antibiotic ran out after ten days.The man only died when the antibiotic ran out after ten days.

86 ~Different types of antibiotics kill different bacteria – but not viruses like those causing flu or the cold. Penicillin now cures diseases such as pneumonia and diptheriaPenicillin now cures diseases such as pneumonia and diptheria

87 Antibiotic action If an antibiotic can inhibit growth of a species of bacteria we say the bacteria is sensitive to that antibioticIf an antibiotic can inhibit growth of a species of bacteria we say the bacteria is sensitive to that antibiotic If an antibiotic has no effect we say that species of bacteria is resistant to the antibiotic.If an antibiotic has no effect we say that species of bacteria is resistant to the antibiotic. There is no one antibiotic that works against all species of bacteriaThere is no one antibiotic that works against all species of bacteria

88 AntibioticPneumoniaTuberculosisTyphoidDiptheria penicillin Streptomycin- -- Tetracycline Cloram- phenical = very effective + + = effective + = slightly effective - = no effect

89 Different antibiotics have different methods of destroying bacteria:Different antibiotics have different methods of destroying bacteria: –Some destroy bacterial cell walls –Some burst the cell membrane –Some interfere with the bacterial cell’s chemical reactions

90 Different types of antibiotics (Activity 4.3) Different antibiotics are effective against different infections. Antibiotic Infection which it treats penicillin respiratory infections (and many others) aminoglycosides Eye and skin infections fluoroquiniles Gonorrhoea (STD) cephalosporinc Urinary infections tetracyclinesacne

91 Choosing the correct antibiotic When a patient has an unknown bacterial infection, a sample of body fluid taken so that the bacteria can be grown on nutrient agar.When a patient has an unknown bacterial infection, a sample of body fluid taken so that the bacteria can be grown on nutrient agar. A multidisc is placed on the agar surface.A multidisc is placed on the agar surface.

92 It is important to have a choice of antibiotics because:It is important to have a choice of antibiotics because: –The person may be allergic to an antibiotic –Bacteria may become resistant to an antibiotic

93 Antibiotic production Antibiotics produced in large fermenters holding 200,00 litresAntibiotics produced in large fermenters holding 200,00 litres Growth conditions are controlled by computer to provide correctGrowth conditions are controlled by computer to provide correct –Temperature –pH –Oxygen concentrations –Food supply –Sterile conditions Antibiotic purified by filtering and solvent extraction

94 Genetic modification Genetic modification is a new technology.Genetic modification is a new technology. It changes the genes found in living things.It changes the genes found in living things. The penicillin gene can be taken from the fungus and put into bacteria.The penicillin gene can be taken from the fungus and put into bacteria. These ‘genetically modified’ bacteria can then produce very large quantities of penicillin.These ‘genetically modified’ bacteria can then produce very large quantities of penicillin. New, more effective antibiotics can also be produced to help fight disease.New, more effective antibiotics can also be produced to help fight disease.

95 Antibiotic production Activity 4.2  Antibiotics were first produced in ___________ by ______________ ____________. They are chemicals which kill ____________. They do not kill _________.  Antibiotics are produced in huge ____________. Growth conditions inside the fermenters are controlled by ____________. If the glucose level in the vessel falls then the ________ will detect this _____________and more ____________ will be added. The antibiotic is _________________ by filtering and _______________ extraction.  ______________ modification is a new ______________ which can alter the _____________ of living organisms. This new technology may be used to produce new _____________ which will be better at fighting_________

96 Antibiotic production Activity 4.2  Antibiotics were first produced in London by Alexander Fleming. They are chemicals which kill bacteria. They do not kill viruses.  Antibiotics are produced in huge fermenters. Growth conditions inside the fermenters are controlled by computers. If the glucose level in the vessel falls then the computer will detect this change and more glucose will be added. The antibiotic is purified by filtering and solvent extraction.  Genetic modification is a new technology which can alter the genes of living organisms. This new technology may be used to produce new antibiotics which will be better at fighting disease.

97 Antibiotic resistance  Some infections have become resistant to antibiotics. This means that the antibiotic is no longer effective.  Staphylococcus aureus is a bacteria that causes abscesses and boils  1940’s this bacterium was sensitive to penicillin so it could be used to treat Staphylococcus aureus infections.

98  Now some strains of this bacteria are resistant to penicillin and it is no longer effective.  These strains have also become resistant to other antibiotics and are known as MRSA. These strains are prevalent in hospitals where infections are easily spread.  This has happened because because antibiotics have been over-used.

99 Public concern People are concerned about the over-use of antibiotics in agriculture and by vets.People are concerned about the over-use of antibiotics in agriculture and by vets. The same antibiotics areThe same antibiotics are used in animals and humans. It is thought that this might result in more resistant strains of bacteria.It is thought that this might result in more resistant strains of bacteria. New antibiotics to which bacteria are not resistant are continually being looked for.New antibiotics to which bacteria are not resistant are continually being looked for.

100 Anti-fungals Some infections are caused by microbes called fungiSome infections are caused by microbes called fungi They are spread from person to person by tiny groups of fungi called spores.They are spread from person to person by tiny groups of fungi called spores. Drugs used to treat these infections are called anti- fungals.Drugs used to treat these infections are called anti- fungals. Anti-fungal treatment slows down or stops fungal growth.Anti-fungal treatment slows down or stops fungal growth.

101 Athlete’s foot This fungi likes to grow areas of the skin which are warm and moist and get little fresh air.This fungi likes to grow areas of the skin which are warm and moist and get little fresh air. Forms an itchy rash between the toes.Forms an itchy rash between the toes. Transferred in shared dressing areas or showers.Transferred in shared dressing areas or showers. Flakes of skin from an infected person are enough to pass the infection onFlakes of skin from an infected person are enough to pass the infection on Treated with antifungal creams or powders.Treated with antifungal creams or powders.

102 Oral thrush Fungal infection of the mouth.Fungal infection of the mouth. Seen as white spots in the mouth.Seen as white spots in the mouth. Common amoung:Common amoung: –Babies –People with ill fitting dentures –Chemotherapy patients –Drug users Treated with antifungal mouth washes or pastillesTreated with antifungal mouth washes or pastilles

103 Ringworm Begins as a patch of itchy skinBegins as a patch of itchy skin Spreads to form spiral shapesSpreads to form spiral shapes Caused by a fungus not a worm!Caused by a fungus not a worm!


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