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1 USE OF STABILIZERS TO IMPROVE THE TEXTURE OF A FERMENTED BEVERAGE ELABORATED FROM MILK AND ACID WHEY Lurdes G. Siberio-Pérez Food Science and Technology.

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Presentation on theme: "1 USE OF STABILIZERS TO IMPROVE THE TEXTURE OF A FERMENTED BEVERAGE ELABORATED FROM MILK AND ACID WHEY Lurdes G. Siberio-Pérez Food Science and Technology."— Presentation transcript:

1 1 USE OF STABILIZERS TO IMPROVE THE TEXTURE OF A FERMENTED BEVERAGE ELABORATED FROM MILK AND ACID WHEY Lurdes G. Siberio-Pérez Food Science and Technology Program May 6, 2010.

2 2 Agenda Introduction Objectives Materials and Methods Results/Discussion Conclusions Recommendations Acknowledgements

3 3 Introduction Due to the decrease in the consumption of milk (Tamine, 2002; Boor, 2001) – Different taste – Longer shelf life – Easy lactose digestibility Pszczola (2008) – Sales increased at a rate of 20% – Fermented or cultured dairy products Yogurt Yogurt drinks

4 4 Fermented Dairy Products Cultured Milk (CFR ) – The food produced by culturing with microorganisms. Cream Milk, partially skim, skim milk Buttermilk, whey – Kefir Acidic mildly alcoholic fermented dairy beverage (Witthuhn et al., 2005). – Kefir-grains – Starter cultures Lactose is used by the mixed cultures to carry out the fermentation process. content/uploads/2008/01/kefir.jpg

5 5 Whey By-product of the manufacture of cheese – Sweet whey – Acid whey 93.4% Water 6.35% Total Solids – Lactose Environmental problem due to biological oxygen demand (BOD) (Paraskevopoulou et al., 2003) Its disposal into rivers and onto fields promotes bacterial growth and causes oxygen depletion of water and soil (Ghaly and Ramkumar, 1999). 269e20120a5a134e2970b-500pi

6 6 Whey cont. Puerto Rico (2005) – 4 million tons of acid whey where produced (ORIL, ). Further whey utilization (Gallardo-Escamilla et al., 2005; González-Martínez et al., 2002) – Manufacture of fermented beverages Use of potentially valuable nutrients Reduction of environmental pollution

7 7 Whey cont. Itara-Rodríguez (2007) – “Elaboration of a Fermented Beverage From Acid Whey and Milk” Prepared different fermented beverage formulations Beverages with high proportions of whey – 75% and 50% Low Viscosity – Recommendation Use of stabilizers

8 8 Stabilizers Polysaccharides or Hydrocolloids – Thicken and gel aqueous solutions. – Modify and control the texture – Control syneresis – In cultured milks Control texture and whey separation (Singh and Heldman, 1993) Prevent milk proteins from sedimentation Use of stabilizers that have electrostatic interactions with milk proteins (Tamine, 2006; Ares et al., 2007) – Pectin – Gelatin

9 9 Stabilizers cont. Pectin – Gallardo-Escamilla et al., (2007); studied the characteristics of fermented sweet whey after the addition of several stabilizers. Pectin – Increased the instrumental and perceived viscosity of whey. – Matched the physical viscosity of commercial fermented milk beverages.

10 10 Stabilizers cont. Gelatin – Ares et al., 2007; studied the effect of the addition of gelatin and starch on the rheological and sensory textural properties of plain yogurt. Gelatin – Higher viscosity was observed. – Higher sensory viscosity, creaminess and mouth-feel than starch when added to plain yogurt in the same concentration.

11 11 Objectives Primary Objective – Improve the viscosity of two different fermented beverage formulations with the addition of stabilizers. 75% Whey / 25% Milk 50% Whey / 50% Milk Stabilizers – Pectin – Gelatin

12 12 Objectives cont. Secondary Objectives – Determine which stabilizer improves the viscosity of the fermented beverage formulations. – Evaluate consumer acceptability of the fermented beverage formulations through a sensory analysis.

13 13 Materials and Methods

14 14 Milk Processing pH Fat Content Cream % Skim Milk % Acid Whey %

15 15 Beverage Formulations

16 16 Beverage Formulations cont. Addition of Kefir Starter Culture (2.5g/500ml) Enumeration of Microorganisms, pH and Titratable Acidity Incubation at 25ºC until pH 4.4 ±0.2

17 17 Fermented Beverages

18 18 Results and Discussion Physicochemical characteristics Fat, Total Solids and Protein

19 19 Chemical Composition Fat, Total Solids, and Protein Values in the same column with different letters are significantly different (Tukey test at a=0.05). Values are given as means with standard deviation value.

20 20 Total Solids Theoretical Yield

21 21 Protein Theoretical Yield

22 22 Results and Discussion cont. Physicochemical Characteristics cont. pH and Titratable Acidity

23 23 Physicochemical Characteristics cont. pH and Titratable Acidity Values in the same column with different letters are significantly different (Tukey test at a=0.05). Values are given as means with standard deviation value.

24 24 Results and Discussion cont. Enumeration of Microorganisms Lactic Acid Bacteria and Yeast

25 25 Lactic Acid Bacteria and Yeast Values in the same column with different letters are significantly different (Tukey test at a=0.05). Values are given as means with standard deviation value.

26 26 Lactic Acid Bacteria 1 S.A.- Witthuhn et al., 2004; 2 S.A. Loretan et al., 2003; 1TW Kuo and Lin, 1999; 2TW Liu and Lin, 2000 * grains collected from different households + Current investigation; ++ Formulations with different whey-milk proportions Enumeration of lactic-acid bacteria isolated from kefir elaborated with kefir grains from different locations.

27 27 Yeast 1 S.A. Witthuhn et al., 2004; 2 S.A. Loretan et al., TW Kuo and Lin, 1999; 2TW Liu and Lin, 2000 grains collected from different households + Current investigation; ++ Formulations with different whey-milk proportions Enumeration of yeast isolated from kefir elaborated with kefir grains from different locations.

28 28 Results and Discussion cont. Rheological Properties Flow Behavior Index and Consistency Index

29 29 Flow Behavior Index and Consistency Index n and K Values in the same column with different letters are significantly different (Tukey test at a=0.05). Values are given as means with standard deviation value.

30 30 Flow Behavior Index (n) Flow behavior index for lactic beverages and yogurt in different studies. 50/50 (0.367) 50/50 P (0.365) 50/50 G (0.297) lower n

31 31 Consistency Index (K) Consistency index for lactic beverages and yogurt in different studies. Control (3.74 Pa*s n ) 75/25 P (2.62 Pa*s n )

32 32 Apparent viscosity estimates based on the Power Law Model for the beverage formulations (T=5ºC)

33 33 Results and Discussion cont. Sensory Analysis

34 34 Results and Discussion cont. Sensory Analysis *P-value - <0.05

35 35 Conclusions When compared to the control – Rheology (n and K) 75/25 G showed more similarity – Apparent Viscosity Curves 75/25 G showed a higher viscosity than all other formulations. The use of gelatin improved the viscosity of a fermented beverage prepared with a 75% whey proportion.

36 36 Conclusions cont. Sensory Analysis – Panelists did not find differences between: control, 75/25 G and 50/50 P – In the elaboration of the fermented beverages, whey can be used in proportions of 75% when stabilized with gelatin, and 50% when stabilized with pectin.

37 37 Conclusions cont. The addition of stabilizers improved the viscosity of the fermented beverage formulations. Whey can be used in higher proportions than those recommended by Itara-Rodríguez (2007). This could help reduce the amounts of whey disposed by cheese manufacturers and would help resolve the problem the dairy industry confronts today.

38 38 Recommendations Addition of flavors and sweeteners to make the beverages more acceptable to the consumers. Microbiological analysis to determine the shelf life of the final product.

39 39 References Aportela-Palacios, A., Sosa-Morales, M., and J. Velez-Ruiz Rheological and Physicochemical Behavior of Fortified Yogurt, with Fiber and Calcium. Journal of Textural Studies 36: Ares, G., Concalvez, D., Perez, C., Reolon, G., Segura, N., Lema, P., and A. Gambaro Influence of gelatin and starch on the instrumental and sensory texture of stirred yogurt. International Dairy Journal 60: Boor, K ADSA Foundation Scholar Award. Fluid Dairy Product Quality and Safety: Looking to the Future. Journal of Dairy Science 84:1-11. Davis, J. and F. Macdonald Rishmond’s Dairy Chemistry. Charles Griffin and Company Ltd., London, England. Domagala, J Sensory Evaluation and Rheological Properties of Yogurts Prepared from Goat, Cow, and Sheep Milk. Electronic Journal of Polish Agricultural Universities 11:1-8 Fox, P Developments in Dairy Chemistry-Lactose and Minor Constituents, Applied Science Publishers Ltd., London, England. Gallardo-Escamilla, F., Kelly, A., and C. Delahunty. 2005a. Influence of Starter culture on Flavor and Headspace Volatile Profiles of Fermented whey and whey Produced from Fermented Milk. Journal of Dairy Science 88: Gallardo-Escamilla, F., Kelly, A., and M. Delahunty Mouthfeel and flavour of fermented whey with added hydrocolloids. International Dairy Journal 17: Ghaly A., and D. Ramkumar Controllin the pH of Acid Cheese Whey in a Two-Stage Anaerobic Digester with Sodium Hydroxide. Energy sources 21: Gonzalez-Martinez, C., Becerra, M., Chafer, M., Albors, A. Carot, J., and A. Chiralt Influence of substituting milk powder for whey powder on yogurt quality. Trends Food Science and Technology 13:

40 40 References cont. Itara-Rodríguez, L Elaboración de una bebida fermentada a partir de un suero ácido y leche. Thesis M.S. University of Puerto Rico, Mayaguez, P.R., 15-16, 53, pp Jennes, R., and S. Patton Principles of Dairy Chemistry. John Wiley and Sons, Inc., New York, N.Y Kuo, C., and C. Lin Taiwanese kefir grains: their growth, microbial and chemical composition of fermented milk. Australian Journal of Dairy Technology 54: Liu, R.J., and C.W. Lin Production of Kefir from Soymilk With or Without Added Glucose, Lactos, or Sucrose. Food Microbiology and Safety 65: Loretan, T., Mostert, J.F. and B.C., Viljoen Microbial flora associated with South African household kefir. South African Journal of Science 99: Oficina Reguladora de la Industria Lechera (ORIL), Departamento de Agricultura Informe Anual Estado Libre Asociado de Puerto Rico. Paraskevopoulou, A., Athanasiadis, G., Blekas, G., Koutinas, A., and M. Kanellaki Influence of polysaccharide addition on stability of cheese whey kefir-milk mixture. Food Hydrocolloids 17: Penna, A., Sivieri, D., and M. Oliveira Relation between quality and rheological properties of lactic beverages. Journal of Food Engineering 49:7-13. Pszczola, D What’s Yonder for Yogurt. Food Technology, 62:3: Singh, P., and D. Heldman Introduction to Food Engineering. Academic Press, San Diego, pp. Tamine, A Fermented Milks. Blackwell Science, Oxford, pp. Tamine, A Fermented milks: a historical food with modern applications-a review. European Journal of Clinical Nutrition. 56:S2-S15. Walstra, P. and R. Jenness Dairy Chemistry and Physics. John Wiley and Sons, New York, N.Y. Witthuhn, R., Schoeman, T., and T. Britz Isolation and characterization of the microbial population of different South African kefir grains. International Journal of Dairy Technology 57:33-37.

41 41 Acknowledgements God “ Give thanks in all circumstances, for this is God’s will for you in Christ Jesus”. 1 Thessalonians 5:18 USDA-CREES project H-417. To all of those who in one way or another where there to help me in order for me to see a light at the end of the tunnel. To all, I’m truly grateful!

42 42 Questions? ? ¿ ? ? ? ? ¿ ¿ ¿ ?

43 43

44 44 Total Solids

45 45 Protein

46 46 Soluble and Colloidal Salts in Milk Equilibrium between soluble and colloidal salts. – The salt content of milk exists in a dynamic equilibrium. – Any alteration in one salt, or the form in which it exists, causes a shift in the balance and/or form of other salts. – The factor that shows a direct effect on processing is the change in the form of calcium in a milk product. Fox, 1985; Davis and Macdonald 1953; Jenness and Patton 1959; Wasltra and Jenness 1984

47 47 Factors Influencing the Salt Equilibrium Addition of an acid – Colloidal calcium phosphate is solubilized. Addition of an alkali – Soluble calcium phosphate is precipitated in colloidal form. Addition of colloidal phosphate – As colloidal calcium phosphate is added to milk, content of soluble phosphate is lowered, and pH decreases. Fox, 1985; Davis and Macdonald 1953; Jenness and Patton 1959; Wasltra and Jenness 1984

48 48 3 Ca HPO 4 -2 Ca 3 (PO 4 ) 2 + 2H + Higher levels of colloidal calcium phosphate, an overall acid reaction. Fox, 1985; Davis and Macdonald 1953; Jenness and Patton 1959; Wasltra and Jenness 1984


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