Download presentation

Presentation is loading. Please wait.

Published byElfrieda Blair Modified over 2 years ago

1
The Importance of Activator- Repressor Balance in Various Models of Sequestration for Circadian Clocks Roxana Gheorghe ‘15 Mary Fletcher ‘13 Olivia Lang ‘15

2
Circadian Clock Model Per PER BMAL Activate Repress dPer/dt = f 1 (Per, PER, BMAL) dPER/dt = f 2 (Per, PER, BMAL) dBMAL/dt = f 3 (Per, PER, BMAL) … transcription translation

3
Protein Sequestration Per BMAL Activate transcription PER BMAL translation Inactive Complex

4
Different Mathematical Models Explicit Implicit Direct Hill or Transcription Rate Expressions *J Kim, MSB 2012 *

5
Evaluating Models Balanced Proteins -> High Oscillations -Kim and Forger BMAL PER

6
Finding Stoichiometry and Amplitude of a Set of Rate Constants A =.5289 S =.1681.1478

7
Kim and Forger result

8
Trends in Amplitude vs Stoichiometry Goodwin PNF Implicit Sequestration

9
Models Modeled after Repression Model Number of States Leloup & Goldbeter 2003 MammalianExplicit sequestration 16 Mirsky 2009MouseDirect Hill (1)21 Ueda 2000DrosophilaDirect Hill (2)10

10
Trends in Amplitude vs Stoichiometry Leloup & Goldbeter Ued a Mirsky Direct Hill Repression Implicit Sequestration Explicit Sequestration Average Stoichiometry Amplitude of Per mRNA

11
What’s Wrong? Bad measure of stoichiometry Maybe it’s not a sequestration modeling problem

12
Stoichiometry dPer/dt = f 1 (Per, PER, BMAL) = 0 dPER/dt = f 2 (Per, PER, BMAL) = 0 dBMAL/dt = f 3 (Per, PER, BMAL) = 0 … Per PER BMAL Activate Repress Transcription Translation PER fixed BMAL fixed Solve for Per fixed, PER fixed, BMAL fixed PER avg BMAL avg.1681.1478

13
Stoichiometry Ueda Direct Hill RepressionUeda Implicit Sequestration

14
Conclusion The model of repression on its own does not appear to have a clear effect on the simulation Ratio at the fixed point is not always well approximated by the ratio of average concentrations

15
Acknowledgements Thanks to Stephanie Taylor for mentoring us We also thank the rest of the class for their helpful input

Similar presentations

OK

Stochastic Analysis of Bi-stability in Mixed Feedback Loops Yishai Shimoni, Hebrew University CCS Open Day Sep 18 th 2008.

Stochastic Analysis of Bi-stability in Mixed Feedback Loops Yishai Shimoni, Hebrew University CCS Open Day Sep 18 th 2008.

© 2017 SlidePlayer.com Inc.

All rights reserved.

Ads by Google

Ppt on technology used in space to search for life and water Ppt on amplitude shift keying modulation Maths ppt on surface area and volume class 9 Ppt on phonetic transcription Ppt on online hotel management system Games we play ppt on website Ppt on national education policy 1986 mets Ppt on cross docking distribution Ppt on agriculture in indian economy Ppt on history of basketball