Presentation on theme: "Virology review session: first midterm By Philippe Perron Savard."— Presentation transcript:
Virology review session: first midterm By Philippe Perron Savard
Lecture 1 Don’t bother. No questions will be asked.
Lecture 2: replication cycle Cells provide: ribosomes, nucleotides & aa, ATP (energy), membranes. Consequences of virus replication on the cells: –Diverts cell metabolism –Fill up the cells –Escape (lysis for example) –Apoptosis induction
Lecture 2: virus structure Some viruses affect the behaviour of their host to improve transmission A lot of viruses do not cause disease
Lecture 2: virus structure Techniques used to detect and measure viruses: –Electron microscopy –Hemagglutination –Plaque assay –Foci formation Replication cycle
Finding the pfu/ml If a dilution (1/10000) is made and 1 ml of this dilution is plated: we get 50 plaques. How do we solve this: 50 plaques in 1 ml of the dilution= 50 pfu/ml in that dilution. 50 pfu/ml * 10000 = 500 000 pfu/ml in the original solution.
Lecture 3: virus structure One step growth curve Baltimore classification Basics of virus particles Icosahedral and helical symmetry
Lecture 4: ssRNA phages Gene regulation dependent mostly on RNA secondary structure Replication Vs translation problem Replication Transcription of maturation gene
Lecture 5: ssDNA phage ( X174) Displays extensive overlapping in genes. How do we regulate transcription? Concept of frames in transcription Entry Replication through rolling circle Formation of the capsid
T7 phage Entry and DNA winching by RNA pols from the host and the virus. How it regulates transcription of its genes How the T7 RNA pol functions Problems with replication of linear dsDNA pET vectors for protein expression
Lambda phage Genome organisation Understanding gene regulation is crucial How do we repress the lytic cycle? How do we decide between lysis or lysogeny Integration into the bacterial genome and its regulation