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How Are They Similar? A Metabolic Revolution.

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Presentation on theme: "How Are They Similar? A Metabolic Revolution."— Presentation transcript:





5 How Are They Similar?

6 A Metabolic Revolution

7 The Oxygen Revolution

8 Why was the development of photosynthetic lifeforms so revolutionary?

9 With more oxygen, life could move onto land and took on larger, more complex and more active forms

10 Why was this revolution ultimately mutualistic?


12 How Do Photosynthesis & Respiration Compare?

13 Comparative Functions PHOTOSYNTHESIS What: Using the energy of the sun, high potential energy glucose is built from water and carbon dioxide CELLULAR RESPIRATION What: High potential energy of glucose is transferred to the bonds of multiple ATP

14 Comparative Reactants & Products PHOTOSYNTHESIS –6H 2 O + 6CO 2 + Light Energy ->C 6 H 12 O 6 + 6O 2 CELLULAR RESPIRATION (Aerobic) –C 6 H 12 O 6 + 6O 2 -> 6H 2 O + 6CO 2 + ATP

15 Comparative Multi-Step Processes Photosynthesis –Photolysis – excite electrons with radiant energy, generating ATP and splitting water to provide hydrogen –Calvin Cycle –CO 2 is “fixed” by the leaf and combined with H to make glucose. Powered by ATP from photolysis Aerobic Respiration –Glycolysis – split 6 carbon glucose into two 3-carbon pyruvate –Krebs Cycle – breakdown further to 1-carbon CO 2 –Electron Transport Chain – use electrons from Glycolysis and Krebs Cycle to power the synthesis of ATP

16 Comparative Locations


18 Lab: Balance of Photosynthesis & Respiration Purpose: To assess the relative conditions for and rates of photosynthesis and respiration within a single organism Method: Qualitative assessment of pH using Brom-Thymol Blue (an indicator for acids)

19 Elodea canadensis Canadian waterweed Performs both cellular respiration and photosynthesis under the right conditions

20 Key Conceptual Points Aerobic respiration produces CO 2 as waste. CO 2 + H 2 O -> H 2 CO 3 (carbonic acid) The Calvin Cycle of photosynthesis effectively “fixes”/removes CO 2 from the system

21 Tracking The Changes If excess CO 2 enters the system, Brom- Thymol blue will turn yellow, indicating that the solution is now acidic (due to the carbonic acid produced) If CO 2 is removed, the system may shift back from acidic to neutral, indicated by the BTB returning to its normal blue color

22 What do the results mean? Shift to yellow? CO 2 is being produced faster than it is being removed Respiration > Photosynthesis Shift to blue? CO 2 is being removed faster than it is being produced Respiration< Photosynthesis

23 Protocol Place 200mL of H 2 O into two different Ehrlenmeyer flasks Obtain BTB from your instructor and add to each flask (appx. 5mL per flask). Using a straw, exhale into one of the flasks until it is acidified, indicated by a shift to yellow

24 Setting Up The Trials Clean and label your four test tubes Place a sprig of Elodea into test tube 1 and fill the remainder with acidified (yellow) BTB Fill test tube 2 to the top with acidified BTB. Place a sprig of Elodea into test tube 3 and fill the remainder with neutral (blue) BTB Fill test tube 4 to the top with blue BTB

25 Applying the Variable Lab groups 1-3 will place their bottles in the dark Lab groups 4-6 will place their bottles in the light Reassess color of tubes later.

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