Presentation on theme: "Developmental Anatomy"— Presentation transcript:
1Developmental Anatomy Preformation vs Epigenesis: A history.Primary Germ Layers and early organs.Karl von Baer and his laws.Fate MappingEvolutionary Embryology.
2Aristotle: First biologist? The Generation of Animals (350 BCE)Noted Variations of life cycle themes:Some born of Eggs (oviparity)Some from live birth (viviparity)Some from eggs, but hatch in mother (ovoviviparity).Holoblastic- whole egg divided into smaller cellsMeroblastic- half egg divides into smaller cells; rest is yolk sac.
5PreformationBelief that everything is in egg and/or sperm in smaller form.Overtime, organs and essentially the organism grows.Marcello Malphigi- studies chick embryos..notes abundance of structure in embryo and questions epigenesisPredates cell theory
6EpigenesisEmbryonic parts arise from embryonic tissue. Newly formed organs.Kasper Friedrich Wolff provides evidence that embryonic organs arise from tissue that has no adult counter part!Sets the foundation for Germ layer theory.
7“Developmental Force” Immanuel Kant and Johann Friedrich Blumenbach (mid to late 1700s).Mystical force leads to development of embryos.This “force” is heritable from germ cells and highly susceptible to change.Mendel’s work almost a century later…Developmental force= Genetics?
8Using the microscope revolutionizes development Christian Pander, Heinrich Rathke, and Karl Ernst von Baer.Identify the three germ layers:EctodermMesodermEndoderm
10Triploblastic and diploblastic Organisms These organisms use all three germ layers.Some organisms only use two germ layers (lack mesoderm):Porifera (sponges)Cnidarians (sea anemones, hydra, jellyfish)Ctenophores (comb jellies)
12Christian Pander Work with Chick Embryos. Supports epigenesis by showing that tissues worked together to form organs.Discovers tissue interaction-Induction:No tissue can form organs alone, must interact with other tissues in order to for organogenesis to occur.
13Heinrich Rathke Discovered pharyngeal arches. In fish, these arches give rise to gill apparatusIn humans, form jaw, ears, vertebrate skull.
14Karl Ernst von Baer “Father” of Developmental Biology Expanded Pander’s studies of Chick Embryos.Identifies notochord- rod of dorsal-most mesoderm tissue.Notochord divides embryo in right and left sides, instructs ectoderm above it to differentiate into nerve tissue.
15von Baer lawsRule 1: The general features of a large group of animals appear earlier in development than do the specialized features of a smaller group.All vertebrates have similar structures during development: gill arches, notochord, primitive kidneys.Very similar shortly after gastrulation.
16von Baer’s lawsRule 2: Less General characters are developed from the more general, until finally the most specialized appear.All vertebrates have skin, later skin becomes feathers for birds, scales for reptiles, hair and nails for mammals
17von Baer’s LawsRule 3: The embryo of a given species, instead of passing through the adult stages of lower animals, departs more and more from them.Visceral clefts of embryonic birds/mammals do not ressemble gill slits of adult fish.
18von Baer’s LawsRule 4: Therefore, the early embryo of a higher animal is never like a lower animal, but only like its early embryo.We never pass through a developmental stage similar to adult fish or birds.
20The Cell theory and Fate mapping Matthias Schleiden and Theodor Schwann in formalize the Cell theory: all living organisms are composed of cells!Embryologists followed this movement and began tracking cell movement during development!
25How do you fate map cells? Observing live embryos!Edwin G ConklinStill used as primary fate mapping technique.Zebra Fish will allow you to do this!
26How do you fate map cells? Vital Dye MarkingVital dyes stain the cells but don’t kill them.Dead cells tell no tales…unless you study apoptosis.
27How do you fate map cells? Radioactive/ Fluorescent labelingRadioactive label: Transplant of graft from radiolabeled embryo to unlabeled embryo. Silver stain!Fluorescent labeling: Inject fluorescent dye into cells of embryo.
28How do you fate map cells? Genetics!Use cells from another organism that contain a easily detectable marker!
29Darwin: Embryology’s biggest fan! Saw Embryology as support for his theories.“Community of embryonic structure reveals community of descent”This is shown in Homologous structures, such as the forelimb of human arms vs. wings of a bird!What would be an analogous structure?
30Evolutionary change is due to developmental change.. Ontogeny causes phylogeny?Human fingers versus Bat wing!