2 Founded in 2001 Sacace Biotechnologies s.r.l., is an innovative Italian company, located in Como, Lombardia 2001-2009: Caserta, Campania region Now: Como, Lombardia region
3 Our Mission To supply the best products to the international diagnostics and pharmaceutical industry, as well as to hospitals and laboratories through a global network of distributors located in more than 60 countries all over the world.
SaCycler™-96 Features 4 or 5 channels multiplexing for discrimination of up to five targets in a single reaction well Strong flexibility thanks to the 96-well format suited for standard PCR microplates, test tubes and strips Possibility to use tubes with different heights with regulation of the matrix block Optimal signal/noise ratio and absence of crosstalk ensured by the unique design of the optical track including a separate light source for each channel and a matrix CCD camera Light emitting diodes (LED) as a light source with a lifetime of about 100,000 hours that does not require maintenance
SaCycler™-96 Features Wide dynamic range of detection using multiple exposure method, which leads the optimization of signal registration conditions to a whole new level, greatly simplifying or even eliminating the need for fluorescence settings Main applications are Real-Time quantitation, single nucleotide polymorphisms (SNPs) genotyping, melting curve and gene expression analysis Instrument comes with the most used Sacace protocols already installed in the software minimizing possibility of error during programming.
SaCycler™-96 Characteristics Thermal block format 96 test tubes of 0.2 ml (12 х 8) Test tube type 0.2-ml test tubes for PCR (individual, in strips, 8 pieces each or a holder 12 x 8) Range of thermal block temperature control 0 C…100°С Resolution of temperature setting 0.1°С Absolute accuracy of temperature maintenance ±0.2°С Uniformity of thermal block temperature ±0.15 °С Maximum heating rate of the thermal block within temperature range of 4…99 °С 3.5 °С /s Maximum cooling rate of the thermal block within temperature range of 99….55 °С 2.5°С /s “Hot cover” temperature 105°С 1°С Actuating device of the thermal block Peltier elements Excitation source Light-emitting diode (LED) Detector CCD (charge coupled device) -matrix Number of the fluorescence measurement channels 4 or 5 Excitation/detection wave length 470/525, 532/570, 585/633, 633/670, 690/750
SaCycler™-96 Comparison ABI 7500BioRad CFX96 Qiagen RotorGene QSaCycler-96 Thermal SystemPeltier air cooledPeltier Average ramp rate (°C/s)1.63.315-203.3 Temperature accuracy (°C)±0.25±0.2±0.25±0.2 Uniformity of thermal block temperature (°C)±0.5±0.4±0.01±0.15 Range of temperature (°C)4-1000-100ambient-990-100 Optical System Tungsten halogen lampLED Excitation spectrum5 filters450-684 nm365-680470-750 DetectorCCD cameraPhotodiodesPMTCCD camera Emission filters/channels5664 or 5 Compatible dyes FAM, JOE, VIC, NED, TAMRA, Rox, TexasRed, Cy3, Cy5 FAM, JOE, HEX, R6G, ROX, TexasRed, Cy5, Cy5.5 Format96-well plates rotor disk96-well plates Test tube type (ml)0.2 0.1 - 0.20.2 Supported volumes (µl)20-100up to 5010-5010-100 Weight (Kg)6421.41427