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GMS provides “One stop solution” Sacace Biotechnologies Srl “Complete qPCR Solutions”

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Presentation on theme: "GMS provides “One stop solution” Sacace Biotechnologies Srl “Complete qPCR Solutions”"— Presentation transcript:

1 GMS provides “One stop solution” Sacace Biotechnologies Srl “Complete qPCR Solutions”

2 2 Founded in 2001 Sacace Biotechnologies s.r.l., is an innovative Italian company, located in Como, Lombardia 2001-2009: Caserta, Campania region Now: Como, Lombardia region

3 3 Our Mission To supply the best products to the international diagnostics and pharmaceutical industry, as well as to hospitals and laboratories through a global network of distributors located in more than 60 countries all over the world.

4 Sacace Worldwide

5 5 Functions Research & Development Manufacturing Logistics Marketing Certifications (ISO 9001:2008 13485:2012, CE Mark - Directive 98/79 EC) International external quality control

6 Hepatitis Viruses (Quantitative tests for HCV, HBV, HDV, HGV, HAV detection) Human Papilloma Viruses (Screening, Typing, Quantitative detection of Low & High Risk genotypes) STD (Multiplex detection of Chlamydia, Neisseria, Trichomonas, Mycoplasma, Treponema, Candida ….) Herpes Viruses (Quantitative Multiplex detection of CMV, EBV, HHV6, HSV, VZV) Our PCR Kits

7 TORCH infections (Toxoplasma, Rubella, Parvovirus) Respiratory infections (Tuberculosis, Influenza, hRSV, Bordetella, Pseudomonas, Legionella) Neurological infections (Enteroviruses, N.meningitidis, H.influenza, Str.pneumoniae) Gastrointestinal infections (Shigella, Salmonella, E.coli, Rotavirus, Astrovirus, Norovirus, H.pylori) Our PCR Kits

8 Single Nucleotide Polymorphisms (SNP): Cardio Trombophilia Panel (FII, FV, FVII, FXIII, FGB) Cardio Hypertension Panel (ADD, AGT, NOS....) Folates Methabolism Panel (MTHFR, MTR, MTRR) Haemochromatosis Panel (HFE 187, HFE193, HFE845) Warfarin Panel (CYP2C9, CYP4F2, VKORC1) Our PCR Kits

9 Oncologic diseases (Mbcr-ABL, BRCA) Genetically Modified Organisms (Soya, Corn, Rice, Tomatoes) Veterinary kits (CPV, PPV, CSVF, BVDV, ASF, Brucella, TGEV...) RNA/DNA extraction kits Our PCR Kits

10 Our Real Time PCR kits can work on multiple instruments: Qiagen Rotorgene TM Bio-Rad iQ5 TM Cepheid SmartCycler TM Applied Biosystems 7300/7500/StepOne TM Stratagene MX3005P TM Roche LightCycler 2.0 TM Bioer LineGene-K TM SaCycler-96 TM from Sacace Biotechnologies...more to come Our PCR Kits

11 SaCycler™-96

12 SaCycler™-96 Features 4 or 5 channels multiplexing for discrimination of up to five targets in a single reaction well Strong flexibility thanks to the 96-well format suited for standard PCR microplates, test tubes and strips Possibility to use tubes with different heights with regulation of the matrix block Optimal signal/noise ratio and absence of crosstalk ensured by the unique design of the optical track including a separate light source for each channel and a matrix CCD camera Light emitting diodes (LED) as a light source with a lifetime of about 100,000 hours that does not require maintenance

13 SaCycler™-96 Features Wide dynamic range of detection using multiple exposure method, which leads the optimization of signal registration conditions to a whole new level, greatly simplifying or even eliminating the need for fluorescence settings Main applications are Real-Time quantitation, single nucleotide polymorphisms (SNPs) genotyping, melting curve and gene expression analysis Instrument comes with the most used Sacace protocols already installed in the software minimizing possibility of error during programming.

14 SaCycler™-96 Characteristics Thermal block format 96 test tubes of 0.2 ml (12 х 8) Test tube type 0.2-ml test tubes for PCR (individual, in strips, 8 pieces each or a holder 12 x 8) Range of thermal block temperature control 0 C…100°С Resolution of temperature setting 0.1°С Absolute accuracy of temperature maintenance ±0.2°С Uniformity of thermal block temperature ±0.15 °С Maximum heating rate of the thermal block within temperature range of 4…99 °С 3.5 °С /s Maximum cooling rate of the thermal block within temperature range of 99….55 °С 2.5°С /s “Hot cover” temperature 105°С  1°С Actuating device of the thermal block Peltier elements Excitation source Light-emitting diode (LED) Detector CCD (charge coupled device) -matrix Number of the fluorescence measurement channels 4 or 5 Excitation/detection wave length 470/525, 532/570, 585/633, 633/670, 690/750

15 SaCycler™-96 Comparison ABI 7500BioRad CFX96 Qiagen RotorGene QSaCycler-96 Thermal SystemPeltier air cooledPeltier Average ramp rate (°C/s)1.63.315-203.3 Temperature accuracy (°C)±0.25±0.2±0.25±0.2 Uniformity of thermal block temperature (°C)±0.5±0.4±0.01±0.15 Range of temperature (°C)4-1000-100ambient-990-100 Optical System Tungsten halogen lampLED Excitation spectrum5 filters450-684 nm365-680470-750 DetectorCCD cameraPhotodiodesPMTCCD camera Emission filters/channels5664 or 5 Compatible dyes FAM, JOE, VIC, NED, TAMRA, Rox, TexasRed, Cy3, Cy5 FAM, JOE, HEX, R6G, ROX, TexasRed, Cy5, Cy5.5 Format96-well plates rotor disk96-well plates Test tube type (ml)0.2 0.1 - 0.20.2 Supported volumes (µl)20-100up to 5010-5010-100 Weight (Kg)6421.41427

16 Sacace thermalcycling protocols UROGENITAL + CMV + EBV + TORCH + HPV INFECTIONS Chlamydia trachomatis Real-TM Neisseria gonorrhoeae Real-TM Treponema pallidum Real-TM Mycoplasma genitalium Real-TM Ureaplasma parvum, Ureaplasma urealyticum Real-TM Mycoplasma hominis Real-TM Gardnerella vaginalis Real-TM HSV I,II Real-TM CMV Real-TM Qual CMV Real-TM Quant EBV Real-TM Qual EBV Real-TM Quant Trichomonas vaginalis Real-TM Candida albicans Real-TM Chlamydia trachomatis / Ureaplasma spp. / Mycoplasma genitalium Real-TM Chlamydia trachomatis / Ureaplasma spp. / Mycoplasma hominis Real- TM Neisseria gonorrhoeae / Chlamydia trachomatis / Mycoplasma genitalium Real-TM HSV I,II Typing Real-TM Ureaplasma parvum / Ureaplasma urealyticum Real-TM Trichomonas vaginalis /Neisseria gonorrhoeae Real-TM Chlamydia trachomatis / Ureaplasma spp. Real-TM Chlamydia trachomatis / Mycoplasma genitalium Real-TM Candida albicans / Candida glabrata / Candida krusei Real-TM HSV / CMV Real-TM Mycoplasma hominis / Gardnerella vaginalis Real-TM Ureaplasma parvum / Ureaplasma urealyticum / Mycoplasma Real-TM Trichomonas vaginalis / Neisseria gonorrhoeae /Chlamydia trachomatis Real-TM Neisseria gonorrhoeae / Chlamydia trachomatis Real-TM Pseudomonas aeruginos Real-TM Pneumocystis jirovecii Real-TM CMV/EBV/HHV6 Quant HHV6 Real-TM Quant Rubella Real-TM Toxoplasma Real-TM Parvovirus B19 Real-TM Quant HPV High Risk Screening Real-TM Quant HPV High Risk Typing Real-TM HPV 6/11 Real-TM HPV 16/18 Real-TM 38 different kits

17 Sacace thermalcycling protocols NEUROLOGICAL Poliovirus Real-TM NHS Meningitidis (N. meningitidis, H. influenzae, Str. pneumoniae) Real-TM INTESTINAL INFECTIONS Norovirus/Astrovirus/Rotavirus Real-TM Shigella/Salmonella/Campylobacter Real-TM EHEC Real-TM Helicobacter pylori Real-TM RESPIRATORY INFECTIONS Influenza A/B Real-TM Influenza A Real-TM Influenza A H5N1 Real-TM Influenza A H1N1 Real-TM Influenza A H1N1/H3N2 Real-TM Influenza A H5/H7/H9 Real-TM hRSV Real-TM MTB INFECTION MTB Real-TM MTB Diff Real-TM 2 different kits 7 different kits 4 different kits

18 Thanks for your attention

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