Presentation on theme: "Determination of the Antimicrobial Activity of Plectranthus amboinicus and Plectranthus verticillatus extracts against Escherichia coli and Staphylococcus."— Presentation transcript:
1Determination of the Antimicrobial Activity of Plectranthus amboinicus and Plectranthus verticillatus extracts against Escherichia coli and Staphylococcus aureusBy Katrina GrauMentor Dr. Carolyn MathurDepartment of Biology, York College of PennsylvaniaP. amboinicusP. verticillatusIntroductionMethodsResultsResultsResultsResultsIntroductionMethodsExtraction MethodDried LeavesApproximately 2 weeksPulverized LeavesApproximately 4g leaf matter95% EthanolVortexed with glass beadsSoaked for approximately 24 hoursEvaporated for approximately 48 hoursResuspended in Sterile WaterPlectranthus GenusFound in Tropical Africa, Asia, and AustraliaIncludes over 300 speciesMany species have medicinal usesanti-inflammatoryrespiratory infectionsskin infectionsE. coliFrequently used in scientific researchLactose fermenting, anaerobic, gram negative rodPart of the human body’s natural microbiotaA cause for multiple health issuesFood poisoningUrinary tract infectionsWater contaminationS. aureusGram positive cocciAssociated with nosocomial infectionsMRSAToxic Shock SyndromePneumoniaRelevanceProvides possible grounds for future antibiotic researchPossibly lead to an explanation for why this genus has such fantastic medicinal usesNo zones of inhibition were observed for any of the agar diffusion platesActivity AssaysMinimum InhibitoryConcentration (MIC) MethodConclusionP. amboinicus and P. verticillatus extracts inhibited S. aureus at titers of 2 and 16, respectively, using the Minimum Inhibitory Concentration (MIC) methodNeither plant extracts inhibited E. coli using the MIC methodThe Agar Diffusion Plates method indicated no antimicrobial activity against either bacterium for both P. verticillatus and P. amboinicusMIC method was more sensitive than the Agar Diffusion Method for detecting inhibition in these experiments2-fold Serial DilutionPlant Extract and NutrientInoculated WellsMacFarland Standard of 1ObjectivesIncubatedApproximately 24 hoursDetermine a TiterTo determine if P. amboinicus and/or P. verticillatus extracts exhibit antimicrobial activity against E. coli and/or S. aureusTo measure the degree of antimicrobial activity, if present, for each extractAgar Diffusion MethodPlate Extract Drenched DisksTriplicate on each plateIncubatedApproximately 24 hoursInoculate PlatesMeasure Zones of InhibitionLiteratureAcknowledgmentsBauman, Robert Microbiology: with diseases by Taxonomy. 2nd ed. Pearson Education Inc., San Francisco, CA.De Castro-Ontengco, Delia Screening for the Antibacterial Activity of Medicinal Plants. MicrobeLibrary.Diniz Gurgel, Ana Pavla A., da Silva, Jackeline G., Grangeiro, Ana Ruth S., Oliveira, Danielli C., Lima, Cynthia M.P., da Silva, Aldo C.P., Oliveira, Rinalda A.G., Souza, Ivone A In vivo study of the anti-inflammatory and antitumor activities of leaves from Plectranthus amboinicus (Lous.) Spreng (Lamiaceae). Journal of Ethnopharmacology. 125(Egwaikhide, P.A., and Gimba, C.E Analysis of the Phytochemical Content and Anit-microbial Activity of Plectranthus glandulosis Whole Plant. Middle-East Journal of Scientific Research. 2(3-4):Murthy, Pushpa S., Ramalakshmi, K., and Srinivas, P Fungitoxic activity of Indian borage (Plectranthus amboinicus volatiles. Food Chemistry. 114:Special thanks to Dr. Carolyn Mathur for all her advice and help in the idea, design, and execution of this experiment.Thanks to Professor Daly for supplying the plant materials used in this research.Great appreciation to Jen Woolfenden, Lauren Rebuck, and Kelli Braightmeyer who helped with the preparation for this research.