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Fish Cell Biology/Genetics Basics Dr. Craig Kasper HCC Brandon.

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Presentation on theme: "Fish Cell Biology/Genetics Basics Dr. Craig Kasper HCC Brandon."— Presentation transcript:

1 Fish Cell Biology/Genetics Basics Dr. Craig Kasper HCC Brandon

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10 Zebra Fish Larval Development We thank Leon Browder & Laurie Iten (Ed.) Dynamic Development for their gracious use of material seen in slides We thank Leon Browder & Laurie Iten (Ed.) Dynamic Development for their gracious use of material seen in slides Leon BrowderLaurie ItenLeon BrowderLaurie Iten We also thank Haffter et al., 1996 and The Company of Biologists for their contributions.We also thank Haffter et al., 1996 and The Company of Biologists for their contributions.

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19 Previous Slide Info… Figure 2. Drawings of zebrafish embryos at 24 hours (A), 48 hours (B,D) and 5 days (C,E) of development. For clarity, the pigmentation pattern is omitted from B and C. It is shown in D and E. Most of the structures that can be seen in a living embryo with a compound microscope are labeled. (From Haffter et al., Reproduced with permission from The Company of Biologists.)

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22 Gregor Mendel: Quick Review You might look at possible outcomes for your fish some day. It would be nice to know what to expect, or how you “got what you got…”You might look at possible outcomes for your fish some day. It would be nice to know what to expect, or how you “got what you got…” Punnett Squares and knowledge of basic genetics will help you determine what traits you select for maybe in your breeding. (At least until you spawn several groups.) More on Punnett Squares shortly…Punnett Squares and knowledge of basic genetics will help you determine what traits you select for maybe in your breeding. (At least until you spawn several groups.) More on Punnett Squares shortly…

23 Introduction As always we have several new terms.As always we have several new terms. Genotype: genes of the individual. Your genetic makeup, if you will.Genotype: genes of the individual. Your genetic makeup, if you will. Phenotype: What you look like, or more precisely, what physical manifestations are observed.Phenotype: What you look like, or more precisely, what physical manifestations are observed. Allele: Alternate forms of a gene which are located in the same position on a pair of chromosomes.Allele: Alternate forms of a gene which are located in the same position on a pair of chromosomes.

24 Fig Three paths: Three paths: Two results?? Two results?? Not really! Not really! Again, new terms… Again, new terms… Dominant allele Dominant allele (capital letter) (capital letter) Recessive Allele Recessive Allele (lower case letter) (lower case letter)

25 Alleles on autosomes Alleles on autosomes Basic Mendelian Genetics Basic Mendelian Genetics and the Punnett Square and the Punnett Square If we “cross multiply,” it If we “cross multiply,” it becomes evident that not becomes evident that not all genotypes result in the all genotypes result in the same phenotype. same phenotype. The example at the left is a The example at the left is a single-trait cross. single-trait cross. Notice the expected Notice the expected phenotypic ratio (3:1). phenotypic ratio (3:1). This will become important This will become important later. later.

26 What happens if we What happens if we mix it up a bit?? mix it up a bit?? Same method, different Same method, different results. results. Now the phenotypic Now the phenotypic ratio is 50:50 (Better odds ratio is 50:50 (Better odds than you’d get in Vegas!) than you’d get in Vegas!)

27 Okay, Emeril, let’s…Okay, Emeril, let’s… “Kick it up a notch!!” Dihybrid crossesDihybrid crosses (and beyond) are (and beyond) are handled similarly. handled similarly. You must be carefulYou must be careful to transfer all your to transfer all your alleles though!! alleles though!! Once this is done,Once this is done, figuring out what figuring out what you have can be you have can be challenging. challenging.


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