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Briefly explain the most common immunological tests in the laboratory & discuss the clinical significance of each amna alotiby 2008-2009.

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Presentation on theme: "Briefly explain the most common immunological tests in the laboratory & discuss the clinical significance of each amna alotiby 2008-2009."— Presentation transcript:

1 Briefly explain the most common immunological tests in the laboratory & discuss the clinical significance of each amna alotiby

2 Recombinant ImmunoBlot Assay

3

4 1 Level 3+ C1 C2 E2 NS3 NS4 NS5 Streptavidin Level 1+ ±Level Control lines HCV Antigens

5

6 Level 3+ C1 C2 E2 NS3 NS4 NS5 Streptavidin Level 1+ ±Level 1

7 1 A sample may be: Positive Positive If at least two lines are ± or higher Level 3+ C1 C2 E2 NS3 NS4 NS5 Streptavidin Level 1+ ±Level

8 Negative: Negative: If all lines are negative or only one line has reactivity of ± (Except NS3). 1 Level 3+ C1 C2 E2 NS3 NS4 NS5 Streptavidin Level 1+ ±Level

9 Indeterminate: Indeterminate: If one HCV Ag line has reactivity of 1+ or higher. If NS3 line reacts with ± or higher and all other antigen are negative. Indeterminate: Indeterminate: If one HCV Ag line has reactivity of 1+ or higher. If NS3 line reacts with ± or higher and all other antigen are negative. 1 Level 3+ C1 C2 E2 NS3 NS4 NS5 Streptavidin Level 1+ ±Level


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