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Use of OPU-IVP and SO at CRV breeding program in the genomic era Erik Mullaart and Sybrand Merton.

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Presentation on theme: "Use of OPU-IVP and SO at CRV breeding program in the genomic era Erik Mullaart and Sybrand Merton."— Presentation transcript:

1 Use of OPU-IVP and SO at CRV breeding program in the genomic era Erik Mullaart and Sybrand Merton

2 Content  Introduction  Genomic Selection  Impact on breeding program  Selection procedures  Delta Nucleus breeding program  Application of OPU-IVP and SO  Insire embryo program Holland Genetics

3 CRV Worldwide

4 Genetic products of CRV  7 million units of dairy and beef semen  CRV exports semen to over 50 countries  Test program:  310 Holstein bulls  60 Red Holstein bulls  35 Fleckvieh bulls  35 Jersey bulls

5 Genomic selection

6 Which calf received the best genes from the parents?

7 Marker selection  In early days: selection on individual genes (direct or via markers)  Now: selection based on many markers spread across the whole genome, i.e. selection on all genetic material =GENOMIC SELECTION (or Genomewide Selection)

8 Genomic Selection, What does it bring us?  Reliable breeding values at young age  higher success rate to get top bulls  lower costs for test proofs ( 60,000 markers for ~ € 200)  shorter generation-interval

9 Effects of Genomic Selection  Increased genetic improvement (30-40%)  Higher genetic level of test sires and embryo’s  Wider portfolio of commercial bulls & higher level  More outcross; less inbreeding  Availability of packs of elite young sires (InSire)

10 Impact on breeding program; selection procedures

11 Use of GS in selection procedures; method implemented by CRV  Step 1:  Genotype proven bulls with reliable EBVs (reference sires)  Find markers with an effect on EBVs  Calculate marker effect for each trait  Step 2:  Genotype selection candidates  Calculate GS Breeding Values (GBVs) with the marker effects  Select the animals with the highest GBVs

12 3 full sisters on Delta. Pedigree ( Oman x Jocko Besne)

13 Impact on breeding program; Delta nucleus breeding program

14 16 years of ‘Delta nucleus’  Test heifers 4381  Selected donors 561  Before Genomic Selection  large number of test heifers per year  emphasis on independent test  With Genomic Selection.  limited number of test heifers  optimal use of reproductive possibilities

15 Delta nucleus  Selection of 800 calves  GS selection  After GS-selection 110 donor heifers  MOET en OPU  embryos per heifer  100 selected heifers tested  Selection of donor cows  ET en OPU => 2500 embryos

16 Application of OPU-IVP and SO

17 Embryo production  IVP sessions: 2000 / year  1.6 embryo/ session  SO: 300 / year  4-5 embryos/ flush

18 Production Logistics  3 x SO at months of age:12 embryos  Inseminate at 17 months  17 x OPU at months:20 embryos  Calving at 26 months embryos Holland Genetics

19 Future Production Logistics  2 x SO at months of age: 8 embryos  10 x OPU at months:12 embryos  Inseminate at 18 months  17 x OPU at months:20 embryos  Calving at 27 months embryos Holland Genetics

20 Working method: SO  D Start protocol FSH 1x  D FSH 2x  D FSH 2x + Prostaglandin with 5th FSH injection  D FSH 2x + Prostaglandin with 6th FSH injection  D FSH 1x  D Heat + AI (2x 8-12 hr / hr)  D Flush + Prostaglandin + Progesterone application  D Remove PRID  D Heat  D D start SO again (D - 12)

21 Working method: OPU-IVP Day -1Ovum Pick Up (OPU) In vitro maturation (IVM) Day 0In vitro fertilization (IVF) Day 1Start in vitro culture (IVC) Day 4Cleavage evaluation Day 6Prognoses embryo production Day 7Embryo evaluation (fresh, frozen) Day 8Final embryo evaluation (frozen)

22 Working method: OPU  OPU twice a week (Mo, Thu)  No superstimulation  (para)veterinarian plus assistant  Pregnant heifers and first parity donor cows Holland Genetics

23 Working method: IVM  Maturation for h, 38.5°C, 20% O2  Volume: transport and maturation in 0.5 ml in tubes Holland Genetics

24 Working method: IVF  Semen: Frozen-thawed Separation semen and extender by gradient centrifugation (90% / 45%)  Volume: wells (0.5 ml),  Incubation for 24 h, 38.5 °C, 20% O2 Holland Genetics

25 Working method: IVC  Medium: SOFaaBSA  Volume: wells (0.5 ml)  Incubation for 6-8 days, 38.5°C, 5% O2 Holland Genetics

26 Working method: IVC Embryos  Day 7 - 8, fresh / frozen  Stage (Morula - Hatched Blastocyst)  Grade 1 and 2 IETS Holland Genetics

27 Working method: Embryo transfer Recipients  Healthy, fertile recipients  Natural cycling  Day 6, 7, 8 or 9 of estrus cycle  <5% rejected recipients  150 contracted farms Holland Genetics

28 Working method: Logistics OPU / IVP  OPU and SO location  Transport COC’s  Central IVP lab  Transport of fresh embryos  Recipient herds  Farmers Holland Genetics

29 Results: pregnancy rates  Fresh IVP embryos:  45% - 55%  Frozen embryos:  IVP 40% - 50%  SO 50% - 60% Holland Genetics

30 Results: Efficiency OPU-IVPMOET  Oocytes:89  Cleavage:5,6(70%)  Embryo:1,6(20%)5 (55%)  Calf:0,8(10%)3 (33%)

31 Insire embryo program

32 Number of InSire-embryos per year  InSire-embryo donors  Delta test heifers (IVP + SO) 2500  Delta donor cows (IVP + SO) 500 Total from Delta program = 3000  Marker selected Bullmothers in West-Europe. (e.g. France, Germany, Belgium, Denmark and the Netherlands1000  Totaal4000 InSire-embryos

33 Costs InSire-embryos  InSire-embryos with option € 325,-  InSire-embryos with segment option € 275,-  CRV claims /buy 15-20% of the calves born  male € 1000  female € 3000

34 Segmentation InSire-embryos  Kg Milk  % Protein  Life span en health  Top exterior

35 InSire-embryos offered by Internet  1) Random embryo selection (most actual combination)  Choice of segment ( 4 )  Embryo price € 275  IVP fresh, IVP and SO frozen  2) Newest InSire embryos (most actual combination)  Choice of combination  Embryo price € 325  IVP fresh, IVP and SO frozen  3) Available InSire embryos (in stock)  Embryo price € 325  IVP and SO frozen

36

37 2) Newest InSire embryos (most actual combination)

38 Benefits to the clients

39 Win-Win situation  Client InSire embryos: - Top class calves for a relative low price  CRV: - increased genetics of bulls and bull dams against an acceptable costs

40 Thank you for your attention!


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