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Andrology and IVF Laboratory Services Charles L. Bormann, PhD IVF/Andrology Laboratory Supervisor.

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Presentation on theme: "Andrology and IVF Laboratory Services Charles L. Bormann, PhD IVF/Andrology Laboratory Supervisor."— Presentation transcript:

1 Andrology and IVF Laboratory Services Charles L. Bormann, PhD IVF/Andrology Laboratory Supervisor

2 Laboratory Regulations Clinical Laboratory Improvement Amendments (CLIA) Food and Drug Administration (FDA) College of American Pathologists (CAP) Society for Assisted Reproductive Technology (SART) Mark P.D. Dow PhD, HCLD (Laboratory Director) Charles L. Bormann MS, PhD (Laboratory Supervisor) Lynn Boehnlein BS, MT,TS (Senior Embryologist) Elizabeth Schleinz BS, MT,TS (Senior Embryologist)

3 Semen Analysis Abstain 2-3 days prior to analysis Collect in an approved sterile cup or condom Do not use lubricants for collection Sample processed within 1 hour of collection

4 Volume Viscosity pH Sperm Concentration Sperm Motility Progression/Speed Round Cells World Health Organization >2ml >20 million/ml >50% Morphology (Krueger) ≥14% Parameters DNA Fragmentation Sperm Viability ≤3 ≤30% ≤5/hpf ≥50%

5 Sperm Motility *Motility *Forward Progression *Velocity

6 Sperm Morphology

7 Sperm DNA Fragmentation <30% DNA Fragmentation

8 Sperm DNA Fragmentation Density Gradient Spin Wash Swim-Up

9 Sperm Viability Tests Viability Stain Pentoxifylline Hypo-Osmotic Swelling (HOS)

10 Donor Sperm Purpose: Offer a chance at pregnancy to those without a male partner, or if male partner’s sperm are absent, unable to fertilize, carry a genetic disease, etc

11 Donor Sperm Photos and personal information also available

12 Inter-Uterine Insemination (IUI) Remove seminal plasma Remove debris (WBC, dead sperm) Enrich highly motile sperm population Wash DGS Centrifuge, remove supernatant Wash Count/Assess Reconstitute Load catheter Centrifuge, remove top layers Semen

13 Intracytoplasmic Sperm Injection (ICSI)

14 Male Pronucleus Female Pronucleus ~ 18h post insemination Fertilization

15 Embryo Development

16 In Vitro Culture The in vitro environment was designed to mimic mother nature

17 Embryo Development 2-Cell 4-Cell 8-Cell Morula Blastocyst Hatching Blastocyst Days 1-3 Days 4-6

18 Day 3 vs. Day 5 Embryo Transfer 2CB3BB 4AB 4AA 8AA

19 Preimplantation Genetic Diagnosis Recurrent Miscarriage Unsuccessful IVF Cycles Unexplained Infertility Advanced Maternal Age Male Factor Infertility Inherited Genetic Disorders

20 Preimplantation Genetic Diagnosis (PGD) Embryo Culture Days 1-3 Blastomere Biopsy Day 3Day 5-6 Blastocyst Culture, Embryo Transfer and Possible Freeze Day 3 Embryos (8-Cell)

21 Assisted Hatching Application  >37 yrs of age  Elevated FSH  Abnormally thick zona pellucida  Frozen/thawed embryos/oocytes  Prior failed IVF

22 Fertility Preservation

23 Sperm Freezing Frozen Indefinitely Simple to Freeze No documented risks from using frozen sperm

24 Egg Freezing -Considered Experimental Option for the preservation of fertility in women with malignant diseases -Option for the preservation of fertility in women with malignant diseases -Not ideal for women wishing to delay childbirth

25 Embryo Freezing -Preserve additional embryos for future cycles -Embryos frozen indefinitely

26 Fresh Embryos From Non-Donor Oocytes< Number of cycles38,37221,70719,0998,865 Percentage of cycles resulting in pregnancies Percentage of cycles resulting in live births Age 2007 SART National Summary: ~5-10% Lower with Frozen Embryos

27 Semen Analysis Identification Name: John Doe DOB: March, 23, 2010 MR#: Identification Labeling Chain of Custody Isolation Verification

28 IVF/ICSI Identification Identification Labeling Chain of Custody Isolation Verification

29 Doctors That Are Happy To See You


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