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1 Contingency Blood Collections MSgt Rick Berg TSgt Magdalena Trujillo Lackland Air Force Base Blood Donor Center.

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Presentation on theme: "1 Contingency Blood Collections MSgt Rick Berg TSgt Magdalena Trujillo Lackland Air Force Base Blood Donor Center."— Presentation transcript:

1 1 Contingency Blood Collections MSgt Rick Berg TSgt Magdalena Trujillo Lackland Air Force Base Blood Donor Center

2 2 Contingency Blood Collections Getting started Collection of Acceptable Unit of blood

3 3 Getting started What do I need to do, how do I begin!

4 4 Getting Started What else will I need to begin a blood drive?

5 5 Conducting the drive! Donor Prep Prescreen, DD 572 Vitals Interview Whole Blood collection Processing Infectious Disease Testing

6 6 Donor Prep Use your list!

7 7 Pre-screen, DD 572 Quick Pre-screen

8 8 Vitals WEIGHT: –> 110 lbs, (50Kg) APPEARANCE: –Generally healthy ARM INSPECTION –No skin lesions at site of venipuncture (Leishmaniasis) –Small Pox TEMP: < 37.5 C ( < F) PULSE: BPM If athletic BP: < 180 mm Hg systolic < 100 mm Hg diastolic **Know Manual B/P** HGB: >12.5 g/dL. HCT: >38%

9 9 Interview Private Interview –Ensure all questions are properly answered/documented to ensure donor is eligible

10 10 Whole Blood Collection – Arm Prep ARM PREP Place tourniquet or BP cuff on arm & inflate to mmHg Select a vein Donor is allergic to iodine – Choraprep Scrub site vigorously 30 seconds

11 11 Whole Blood Collection – Arm Prep Second iodine prep –Begin in center of phlebotomy site –Move outward in a circular manner –Never overlap Allow to stand for at least 30 seconds prior to phlebotomy Do not retouch area, if so, MUST repeat arm prep

12 12 Whole Blood Collection - Venipuncture Hemostat the tubing near the needle or use clamp on auto scale Perform venipuncture Once the needle is in the arm remove the hemostat or clamp Blood should flow freely

13 13 Whole Blood Collection Inadequate blood flow: Gently reposition needle If unable to establish adequate blood flow, discontinue Adequate blood flow: Once blood flow is established, secure: –Tape needle down at the hub –Tape tubing to arm

14 14 Whole Blood Collection Collect pilot tubes Strip the tubing at donor bed Take unit to Lab –Confirm Blood type –Major & Minor Crossmatch –HEP/HIV/RPR screening tests

15 15 Unit Collection Monitor blood volume: ml Max collection-20 min Correct amount reached, place hemostat on line after the Y-connection Use metal clamp to seal line (to stop flow into bag)

16 16 Unit Collection Collect pilot tubes Strip the tubing at donor bed Take unit to Laboratory –Confirm Blood type –Major & Minor Crossmatch –HEP/HIV screening tests –Complete paperwork to issue unit to OR/ER

17 17 Post Donation Care Record: –There is always time for documentation Monitor the donor for any delayed reactions Recovery

18 18 Unit Processing  Blood can be kept at room temperature for up to 8 hours from time of collection - NOTE: Do NOT use ice if whole blood is to be used in emergency situation during contingency operations. Ice will destroy platelets. - If unit is not used within 8 hours- Keep unit in fridge for 24 hrs until it expires

19 19 Unit Testing  Test each unit for: - ABO / Rh Pilot tube or Unit Segment  Infectious Disease Testing - Normally, screening tests MUST be done prior to issuing the blood - Refrigerate serum or plasma & ship to FDA licensed testing facility/Lackland Blood Donor Center

20 20 QUESTIONS?

21 21 Sample submission requirements for sending samples to Lackland BDC Administrative requirements for shipping & Collecting sample requirements for testing per CENTCOM

22 22 Sample submission requirements For each blood/platelet donor, submit 3 bar-code labeled plastic transfer tubes, capped, then Para- filmed, containing: 3 ml plasma 3 ml serum Attach a small ISBT barcode label to each transfer tube. Place lengthwise near the top of the tube. Label each transfer tube as “serum” or “plasma”.

23 23 Sample submission requirements Storage requirements: All samples must be shipped frozen Plasma/serum must be removed from the cells within 3 days of collection Samples may be stored at 1-10 C for up to 8 days before freezing Samples must be frozen within 8 days of collection Do not freeze PPT/SST tubes. When freezing samples, freeze the serum/plasma portion only – and only after centrifugation and separation

24 24 Sample submission requirements Shipping requirements Ship in a Collins box Ship on fresh dry ice - using as much dry ice as will fit in the box, leaving as little air in the box as possible. Frozen samples should arrive at <-20 C Follow up with your shipping company to make sure the shipment has left the terminal

25 25 Sample submission requirements Notification and fax notification of shipments to LBDC the day of the shipment departure Attach the shipping invoice listing each sample number to the , as per current practice

26 26 Sample submission requirements Tracking # Facility Id/ YearSAMPLE ID DATE COLLECTED Specimens Frozen?Date FrozenCOMMENTSFacility Id/ YearSAMPLE ID DATE COLLECTED Specimens Frozen?Date FrozenCOMMENTS ______________________________________ ______________________________________ _________________ SIGNATURE OF SHIPPING OFFICIALSHIPPING DATESIGNATURE OF RECEIVING OFFICIAL RECEIVED DATE ______________________________________ SIGNATURE OF 2ND VERIFIER * No action required if specimens were not frozen prior to shipping.RECEIVING TEMPERATURE_____________°C * If specimens were frozen within 8 days of collection, circle 'Yes'. * If specimens were frozen > 8 days of collection, circle 'Yes' and annotateTotal # of Samples/Specimens Shipped: in comment section '> 8 days'. * Provide additional comments as necessary to communicate integrity of specimens.

27 27 Sample submission requirements Rejection Criteria If less than 3 tubes submitted, or less than 3 ml submitted per tube, the sample will be rejected for all testing Non-frozen samples >8 days old will be rejected for NAT testing. All other tests will be performed Non-frozen samples >14 days old will be rejected for all testing

28 28 Sample submission requirements Rejection criteria Frozen samples >60 days old will be rejected for all testing Samples must have been separated from cells within 3 days of collection and frozen within 8 days of collection and remained frozen throughout transit and arrive frozen If samples are shipped via Landstuhl Regional Medical Center (LRMC), LRMC should denote sample acceptability on arrival at their facility and relay that information to LBDC

29 29 Sample submission requirements Rejection criteria Grossly hemolyzed, contaminated or broken samples will be rejected Samples in glass will be rejected. PPT/SST tubes will be rejected

30 30 Sample submission requirements Shipment arrivalTaking temperature

31 31 Sample submission requirements Specimen shipmentSorting out tubes

32 32 Sample submission requirements Putting specimens in order Waiting for testing

33 33 Sample submission requirements Shipment Paperwork

34 34 Sample submission requirements Rejection criteria Responsibility LBDC will notify submitting site of rejected samples via within one duty day of receipt of samples. The COLLECTION SITE is responsible to re-collect the donors ASAP and re-submit proper samples to LBDC

35 35 Testing requirements for CENTCOM regulations No ABO/Rh and CMV will be completed Antibody screens performed on Galileo HBsAg, HBcAb, HTLV 1/2, HIV 1/2, and HCV on Prism WNV and Ultrio (HIV, HCV, HBV) done on Tigris RPR performed on Olympus (PK 7300)

36 36 LBDC Testing area OlympusGalileo

37 37 LBDC Testing area PrismTigris

38 EMDG Aerial View of 332 EMDG

39 EMDG Trauma BayBlood Bank/Trauma Bay

40 EMDG Aphaeresis CollectionBlood Bank

41 EMDG ChemistryMicrobiology

42 EMDG Main Lab

43 EMDG Emergency RoomShipping Department

44 44 QUESTIONS?


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