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Prostate: Transverse section. Apex Bladder neck Radical Prostatectomy.

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Presentation on theme: "Prostate: Transverse section. Apex Bladder neck Radical Prostatectomy."— Presentation transcript:

1 Prostate: Transverse section

2 Apex Bladder neck Radical Prostatectomy

3 Base/ Bladder Neck Apex Posterior quadrants of alternate slices have been removed and frozen after inking the surface

4 Lateral aspect Seminal Vesicle right left 5R5L 4R4L 3R5L 5R 5L 4R 4L 3R 3L Permanent section permanent section Unfixed tissue for RNA & DNA Frozen section Schematic of how samples of tumor are obtained from a radical prostatectomy specimen, without compromising sampling or diagnostic pathology data Frozen section right left = Prostate carcinoma Fresh, non-frozen tissue for flow sorting

5 Human prostate (lateral view) Seminal Vesicle right left 5R5L 4R4L 3R5L 5R 5L 4R 4L 3R 3L Fixed tissue: In-situ hybridization Frozen tissue: Immunostains Laser capture Obtaining samples of non-tumor tissue from a radical prostatectomy specimen. Frozen section right left = Prostate carcinoma Fresh, non-frozen tissue: cell sorting Permanent section Permanent section Permanent section This block is snap frozen (isopentane in liquid N 2 ), within < 20 minutes of devitalization

6 Prostate (transverse section)

7 Normal prostate parenchyma (high magnification) Basal epithelial cells Secretory (luminal) epithelial cells Fibromuscular stromal cells

8 lymphocytes fibromuscular stroma normal glands Normal prostate parenchyma (low magnification)


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