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Imaging Fascia Renee Stenbjorn, MPA, LMT. My goal: Utilize images to inform the student of the complex structural system of fascia. Your goal?

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Presentation on theme: "Imaging Fascia Renee Stenbjorn, MPA, LMT. My goal: Utilize images to inform the student of the complex structural system of fascia. Your goal?"— Presentation transcript:

1 Imaging Fascia Renee Stenbjorn, MPA, LMT

2 My goal: Utilize images to inform the student of the complex structural system of fascia. Your goal?

3 Historical Definition Fascia: general description, a band of tissue. General meaning: undifferentiated tissue surrounding a more specialized tissue. LANGEVIN, MD, H., HUIJING, PHD, P.. Communicating About Fascia: History, Pitfalls, and Recommendations.International Journal of Therapeutic Massage & Bodywork: Research, Education, & Practice, North America, 2, oct Available at:. Date accessed: 14 Jan http://www.ijtmb.org/index.php/ijtmb/article/view/63

4 Current understanding Forms of connective tissue: Loose & Dense Superficial & Deep Multiple- & Single-layered LANGEVIN, MD, H., HUIJING, PHD, P.. Communicating About Fascia: History, Pitfalls, and Recommendations.International Journal of Therapeutic Massage & Bodywork: Research, Education, & Practice, North America, 2, oct Available at:. Date accessed: 14 Jan http://www.ijtmb.org/index.php/ijtmb/article/view/63

5 Gross Anatomy Photography of anatomical structures: What is it? What do we learn?

6 "Old-School" Histology Gradations of pink, some purples, blues: Procedures: Create a specimen Mount on slides Put drops of solutions on slide for specific period of time. Rinse & mount. Put in a slide box.

7 Blood smear, Biology 101

8 "Old-School" Histology Put in a slide box.

9 What do we learn from histology? Highlights & differentiates: Cells, fibers & tissues Gives insight into cellular structures.

10 Histology of Connective Tissue types: Dense Irregular Abundant Collagen fibers, few cells. What type of tissues?

11 Histology of Connective Tissue types: Dense Regular Abundant Collagen fibers, arranged in same direction, f few cells. What type of tissues?

12 Histology of Connective Tissue types: Areolar Sparsely arranged fibers, not ordered in a predominant direction. Oftern refferd to as "Loose" but Dr. Langevin suggests this label is too mechanical in description

13 Connective Tissue types: Bone

14 Connective Tissue types: Blood

15 Specialized Fascial Structures: Superficial Fascia: aka: hypoderm, hypodermis, stratum subcutaneous, etc. Continuous with the dermis, directly underneath the skin

16 Specialized Fascial Structures: Deep Fascia

17 Specialized Fascial Structures: Muscular Fascial layers: Endo Peri Epi

18 Specialized Fascial Structures: Intermuscular Septa

19 Specialized Fascial Structures: Neurovascular Tract

20 Specialized Fascial Structures: Periosteum

21 How did histology help us differentiate: Regular vs. Irregular Fiber Arrangements Layers of fascia Structural differences

22 Really old school...what Golgi saw

23 VERY toxic...

24 Imaging Cells & Fibers Immunohistochemistry: We can localize molecular structures rather than just cellular structures. Can measure DNA & RNA activity

25 What is different? Process of IHC Fluorescent labeling Co-localization of molecules

26 A Study of Collagen in the Nervous System

27 What is a collagen?

28 How do we know where the collagen is...?

29 Dissecting a human brain... Dr. Liechnetz, MCV

30 Compared to a mouse brain...

31 Understanding Blots What do those dark spots mean?

32 Putting the theory to the test Can we find Collagen XVIII (18) in the brain? Using immunohistochemistry to locate structures.

33 Example: Cerebellum staining Collagen 18 found in Purkinje cells in the cerebellum!

34 Let's take a look: layers of fascia How images help us understand layers of tissue

35 Confocal imaging A method of converting IHC images into 3-D images that further our understanding of tissues.

36 3-D images Z Stack

37 What have we learned from these images?

38 The Neuromuscular Junction Understanding the relationship between nerve & muscle

39 The Extracellular Matrix What's in it? Why do we care? Key to hydration.

40 Fascial Innervation

41 Sensory Mechanism Tensigrity architecture allows cells to sense mechanical changes. These changes result in control of cell function & tissue development Donald Ingber

42 Connective tissue: a body-wide signaling network?

43 Looking even deeper with Electron Microscopy Seeing the textures Understanding the structures

44 The myofascia...

45 Imaging with Ultrasound From treatment to imaging: Principles in imaging with US. What can we see with US: what do these images tell us?

46 Ultrasound for differentiating layers

47 Imaging trigger points!!

48 Video Imaging It's a living tissue.

49 What does all of this tell us about treatment??


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