Presentation on theme: "RCSI Tissue Engineering Research Group Institute of Biomedical Sciences- UFRJ Research Initiative Fergal J. O’Brien, PhD Professor of Bioengineering &"— Presentation transcript:
1 RCSI Tissue Engineering Research Group Institute of Biomedical Sciences- UFRJ Research Initiative Fergal J. O’Brien, PhDProfessor of Bioengineering & Regenerative MedicineDeputy Director of Research, RCSIHead of Tissue Engineering Research GroupDeputy Director, Advanced Materials & BioEngineering Research (AMBER) CentreChair of Executive Committee, Trinity Centre for BioengineeringDept. of Anatomy, Royal College of Surgeons in IrelandTrinity Centre for Bioengineering, Trinity College DublinAMBER, RCSI & TCD@TissueEngDublin
2 Transplantation/allografting (lack of donors/donor tissue) Biomimetic ECM-derived biomaterials provide an opportunity to alleviate problems with traditional techniques to replace diseased or damaged organsTransplantation/allografting (lack of donors/donor tissue)Autografting (lack of tissue)Permanent prosthesis (not permanent)
4 A Viable Alternative to Autogenous Bone HydroxyColl™A Viable Alternative to Autogenous BonePatent: WO 2008/096334Enterprise Ireland Commercialisation Fund POC PC/2007/331 & CFTD TD/2007/112
5 ChondroColl™ Hyaline Cartilage Regeneration Empty Defect ChondroColl Addressing problem of regenerating cartilage> 40, body loses the ability to regenerate cartilage.- Variety of treatments available however expensive, limited availably (only ACI available in the US)No long term success.Restoration of hyaline cartilage on articular surface following implantation with ChondroCollEnterprise Ireland Commercialisation Fund POC PC/2005/226 & CFTD TD/2009/1045
7 Scaffolds as Delivery Systems - Cells- Tissue- Genes (non-viral technologies)Growth factors Platelet-rich plasmaScaffolds for controlled, sustained, transient release
8 Collagen Scaffolds as Drug Delivery Systems (A) Controlled release of BMP from a collagen-based scaffold at two different rates using two different types of polymeric microcapsules (B) SEM image of growth factor-containing microparticles embedded in the collagen matrix of the scaffoldEU and PCT Patent (Pending): O'Brien FJ, Lopez-Noriega A, Quinlan E, Partap S, Kelly H, Cryan SA.Application number: EP (Priority Date, October 15, 2012)López-Noriega et al. (2014) Advanced Healthcare Materials. 3(6): Lopez Noriega et al. (2014) Journal of Controlled Release 10;187: Quinlan et al. (2015) Journal of Controlled Release Jan 28;198C:71-79
9 Gene-activated Scaffolds In VivoHealingCell-based manufacture of therapeuticStem cellNano particle + geneTransfected CellTierney et al. (2012) Journal of Controlled Release 158(2): Tierney et al. (2013) Journal of Controlled Release 165(3): Tierney et al. (2013) Organogenesis. 9(1) Raftery et al. (2013) Molecules 18(5):Curtin et al. (2015) Advanced Healthcare Materials. [Epub ahead of print]
10 Clinical Collaborators University of Sydney (Orthopaedics)University of Aachen (Cadiovascular)Lund University Hospital , Sweden (Orthopaedics)Kyoto University (Oral and Maxillofacial Surgery)Beaumont Hospital (Otolaryngology)Cappagh Hospital (Orthopaedics)Temple Street (Cranio/maxillofacial Surgery & ENT)Mater Hospital (Otolaryngology)Royal Victoria Eye & Ear Hospital (Ophthalmology)Prof. David LittleProf. Stefan JockenhoevelProf. Magnus TagilProf. Kazuhisa BesshoProf. Michael WalshProf. John O’Byrne, Prof. Cathal MoranMr. Dylan Murray, Mr. Alec BlayneyMr. Stephen KieranProf. Conor Murphy, Dr. Billy PowerIndustrial CollaboratorsIntegra Life Sciences, NJ, USAMarigot LtdSurgaColl TechnologiesDr. Simon ArchibaldDr. Denise O’Gorman,Pat Forristal, John GleesonNational Academic CollaboratorsRCSI: School of PharmacyMolecular and Cellular Therapeutics TCD: Trinity Centre for Bioengineering (TCBE)Dept. of Zoology CRANNUCD: Dept. of AnatomyDCU: National Insitiute for Cellular BiotechnologyDept. of Mechanical EngineeringNUI Maynooth: Institute of Immunology Dept. of BiologyNUI Galway: National Centre for Bioengineering ScienceREMEDI University of Limerick: Mech Eng & MSSIDrs. Sally Ann Cryan, Helena KellyProf. Ray Stallings, Caroline Jefferies, Dr. Steve Kerrigan,Dr. Danny Kelly, Dr. Conor BuckleyProf. Veronica Campbell, Dr. Bruce MurphyDr. Paula Murphy Profs. Jonathan Coleman, Ed Lavelle Valeria Nicolosi, Graham CrossDr. Tom FlanaganProf. Martin Clynes, Dr. Finbarr O’SullivanDr. Triona Lally,Prof. Bernard Mahon Dr. Shirley O’DeaProf. Peter McHugh, Dr. Laoise McNamaraProf. Frank Barry, Prof. Tim O’Brien, Dr. Mary Murphy, Dr. David HoeyInternational Academic CollaboratorsColombia University: Dept. of Biomedical EngineeringHarvard Medical School: Dept. of Orthopaedic SurgeryGeorgia Institute of Technology: Institute for BioengineeringWake Forest University: Institute for Regen MedicineColarado State University: Biomedical EngineeringImperial College London: Biomaterials & Regen. MedicineUniversity College London: Inst of OrthopaedicsUniversity of Adelaide: Bone and Joint Research Laboratory Utrecht University: Dept. of Biomedical EngineeringErasmus University, RotterdamJohannes Gutenberg Univ, Mainz: Institute of Pathology, Federal University of Rio de Janeiro (UFUJ)Prof. Chris JacobsProf. David Mooney, Prof. Conor WalshProf. Robert GuldbergProf. Shay SokerProf. Seth DonahueProf. Molly StevensProf. Robert BrownProf. Nick Fazzalari, Dr. Ian ParkinsonProf. Wim Hennink & Prof. Gert StormProf. Gerjo van Osch, Drs. Eric Farrell & Y. BastiaansensProf. James KirkpatrickProfs. Marcia Cury, Marcos Farina, Valeria de Mello Coelho
11 Research Program in Bioengineering and Cellular Therapies Federal University of Rio de JaneiroInstitute of Biomedical Sciences-IBSRio de Janeiro- BrazilResearch Program inBioengineering and Cellular TherapiesValeria de Mello CoelhoAssociate ProfessorBioengineering and Cellular Therapies Research Program CoordinatorLaboratory of Immunophysiology, HeadHistotechniques Core Facility, Head
12 Largest Federal University in Brazil 2nd oldest medical school in Brazil (founded in 1808)Tradition in biomedical research- Institute of Biophysics Carlos Chagas Filho, since 1940s- Institute of Biomedical Sciences, since 1960sFocus on engineering and biomedical sciences(biochemistry, cell and tissue biology, neurobiology and stem cell research)
13 Institute of Biomedical Sciences Federal University of Rio de Janeiro 04 Undergraduate Teaching Programs:Anatomy, Cell biology and Developmental biology, Histology and Pharmacology(Attend all Undergraduate Courses in the biomedical field)02 Graduate Programs:Morphological SciencesPharmacology and Medicinal Chemistry07 Research Programs:Bioengineering and Cellular Therapies, Cell Biology, Cellular Differentiation, Drug development, Glicobiology, Pharmacology and Inflammation, Neurosciences
14 Bioengineering and Cellular Therapies Research Program Mission: the conduction of scientific research aiming at the development of new technologies for regenerative medicine17 ICB INTRAMURAL MEMBERSCLAUDIA BATISTAFELIPE LEITE DE OLIVEIRAHELIO DUTRAJOSE BRITOKATIA ARCANJOKATIA CARNEIROLEONARDO RODRIGUES ANDRADEMARCIA CURY EL-CHEIKHMARCOS FARINA – DEPUTY COORDINATORMARIA ISABEL DORIA ROSSIMICHELLE BOTELHOPAULO HENRIQUE R CASTROPAULO LOUZADARADOVAN BOROJEVICTATIANA SAMPAIOVALERIA DE MELLO COELHO -COORDINATOR10 EXTRAMURAL MEMBERSCHRISTINA TAKYIA- UFRJ- IBCCFPRISCILLA FINOTELLI- UFRJ-FFGLORIA SOARES- UFRJ/COPPELEANDRA BAPTISTA- UFRJ-XEREMMONICA CALASANS MAIA-UFFALEXANDRE ROSSI-CBPFANDRE ROSSI- CBPFMAURO GRANJEIRO – INMETROLEONARDO BOLDRINI- INMETROJACQUES WECKERMANN - INMETROACHIEVEMENTS IN THE LAST 04 YEARS:MORE THAN 200 PUBLICATIONS1 BOOK and 12 BOOK CHAPTERS03 PATENTS31 PhD concluded39 Masters concluded08 post-doctoral concluded23 INTERNATIONAL EXCHANGEVisits/ Cooperation with France, Stanford University, National Institutes of Heath, USA/ Organization of international meetingsBRAZILIAN FINANCIAL SUPPORT:
15 Program Overview: Research focus Radovan BorojevicUFRJ/INMETROMarcos FarinaUFRJJosé Mauro GranjeiroUFF/INMETROAlexandre RossiCBPFMonica CalasansUFFAMABLE, PAOLA ROMINA ; TEIXEIRA, MARCUS VINICIUS ; CARIAS, ROSANA BIZON ; Granjeiro, José Mauro ; BOROJEVIC, RADOVAN . Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly. Stem cell research & therapy , v. 5, p. 53,2014.ROSSI, AL. ; MOLDOVAN, SIMONA ; Querido, W ; ROSSI, A; WERCKMANN, J; ERSEN, OVIDIU ; FARINA, MARCOS. Effect of strontium ranelate on bone mineral: Analysis of nanoscale compositional changes. Micron (Oxford. 1993) , v. 56, p , 2014.Baptista, LS. ; SILVA, KR. ; PEDROSA, CSG. ; AMARAL, R J.F.C. ; BELIZÁRIO, JV ; BOROJEVIC, R ; GRANJEIRO, JOSÉ MAURO . Bioengineered Cartilage in a Scaffold-Free Method by Human Cartilage-Derived Progenitor Cells: A Comparison With Human Adipose-Derived Mesenchymal Stromal Cells. Artificial Organs , v. 37, p , 2013.LOPEZ EO, MELLO A, SENDAO H, COSTA LT, ROSSI AL, OSPINA RO, BORGHI FF, SILVA FILHO JG, ROSSI AM. Growth of Crystalline Hydroxyapatite Thin Films at Room Temperature by Tuning the Energy of the RF-Magnetron Sputtering Plasma ACS Appl Mater Interfaces Oct 9;5(19): Valiense, HB ; Alves, A.T.N.N. ; Mavropoulus, E ; TANAKA, M. ; ROSSI, A. M. ; BARRETO, M. ; Granjeiro, JM ; CALASANS-MAIA, M. D. . In vitro and in vivo evaluation of strontium-containing nanostructured carbonated hydroxyapatite/sodium alginate for sinus lift in rabbits. Journal of Biomedical Materials Research. Part B, Applied Biomaterials , v. 1, p. 1-9, 2015.
16 Felipe LOliveiraTatianaSampaioIsabelRossiHelioDutraChristinaTakiyaLeonardo AndradeLeandra BaptistaPriscilla FinotelliPaulo H R CastroMichelleBotelhoKatiaCarneiroJoseBritoPauloLousadaClaudia BatistaMENEZES, K; NASCIMENTO, MA ; GONÇALVES, JP ; CRUZ, AS; LOPES, DV ; CURZIO, B; BONAMINO, M; DE MENEZES, JL ; BOROJEVIC, R; ROSSI, MID ; COELHO-SAMPAIO, T. Human Mesenchymal Cells from Adipose Tissue Deposit Laminin and Promote Regeneration of Injured Spinal Cord in Rats. Plos One , v. 9, p. e96020, 2014.TAKIYA, CM ; BOROJEVIC, R . Cardiac and renal cell therapies: similarities and differences. Kidney International Supplements, v. 1, p , 2011.BLOISE, F. F. ; OLIVEIRA, F. L. ; NOBREGA, A. F. ; VASCONCELLOS, R. ; CORDEIRO, A. ; PAIVA, L. S. ; TAUB, D. D. ; BOROJEVIC R. ; PAZOS-MOURA, C. C. ; DE MELLO-COELHO, V. . High levels of circulating triiodothyronine induce plasma cell differentiation. Journal of Endocrinology, 2014TRIGO, FMB. ; LUIZON,MR. ; DUTRA, HÉLIO S. ; MAIOLINO, Â ; NUCCI, M ; SIMÕES, BP. Interaction between IL-6 and TNF-α genotypes associated with bacteremia in multiple myeloma patients submitted to autologous stem cell transplantation (ASCT). Leukemia Research Reports, v. 3, p , 2014.BRAND, C. ; OLIVEIRA F. L ; Ricon, L. ; FEMINO, M. L. ; BOLDRINI, L. C. ; Hsu, D. K ; Liu, F.-T. ; CHAMMAS, R. ; BOROJEVIC, R. ; FARINA, M. ; EL-CHEIKH, MC. . The bone marrow compartment is modified in the absence of Galectin-3. Cell and Tissue Research (Print) , v. 346, p , 2011.ANDRADE, LR. ; LINS, U ; FARINA, M ; KACHAR, B ; THALMANN, R. Immunogold TEM of otoconin 90 and otolin relevance to mineralization of otoconia, and pathogenesis of benign positional vertigo. Hearing Research, v. 292, p , 2012.ROSADO DE CASTRO PH, Pimentel-Coelho PM, Gutfilen B, Lopes de Souza SA, de Freitas GR, Mendez-Otero R, Barbosa da Fonseca LM3.Radiopharmaceutical Stem Cell Tracking for Neurological Diseases. Biomed Res Int. 2014;2014:
17 Program overview: research focus 1. BIOMATERIALS FOR TISSUE REPAIR: production and characterization of biomaterials for application in the repair of various tissues such as skin, nerve, bone and cartilage;2. CHARACTERIZATION OF MINERAL PARTICLES: The chemical, biophysical and morphological characterization of nanoparticles and of crystals obtained upon experimental biomineralization;3. CONTROL OF CELL PROLIFERATION AND DIFFERENTIATION: aiming at an efficient manipulation of stem or progenitor cells for use in cell therapy;4. IMMUNOMODULATION STUDIES associated to cell therapy and to natural processes such as aging, degenerative diseases or cancer;5. THE UNDERSTANDING OF THE ROLE OF TISSUE MICROENVIRONMENTS as a determinant of cell behavior in several pathophysiological processes.
18 Program overview: annual activities Month seminars (at UFRJ, InMetro, CBPF, UFF):Thesis presentationIntramural and Extramural ResearchersInternational Researchers . Visiting ResearchersAnnual WorkshopPresentation of projects under development by Members of the Bioengineering Researcher programTo stimulate collaboration and plan organization of meetings and application to Research grantsAnnual discipline at the Graduate Program in Morphological Sciences: Bioengineering and Tissue Repair (2 weeks- Lectures and scientific papers presentation) – participation of all Program members and International speakersIn 2015 – will be offered to the undergraduate courses of Biophysics, Nanotechnology, and Biomedical Sciences - at UFRJ
19 2014RONALDO AMARALPhD studentFERGAL O`BRIENSCIENTIFIC KNOW-HOWBRAZILIAN GROUPIRISH GROUPPLATELET-RICH PLASMASCAFFOLDSCELL BIOLOGYCARTILAGE TISSUE ENGINEERINGBASIC SCIENCEAPPLIED SCIENCEPlatelet-rich plasma released differently stimulates cellular commitment towards the chondrogenic lineage according to concentration. Ronaldo JFC do Amaral, Amos Matsiko, Marcel RP Tomazette, Wanessa KR Rocha, Eric Cordeiro-Spinetti, Tanya J Levingstone, Marcos Farina, Fergal J O’Brien, Marcia C El-Cheikh, Alex BalduinoSUBMITTED AT JOURNAL OF TISSUE ENGINEERING
20 2014 AMOS MATSIKO MARCIA CURY EL-CHEIKH SCIENTIFIC KNOW-HOW IRISH GROUPBRAZILIAN GROUPSCAFFOLDSIMMUNOLOGY3D CELL CULTUREEPIGENETICSAPPLIED SCIENCEBASIC SCIENCEMyeloid differentiation in collagen-hyaluronic acid scaffolds under epigenetic control Mariana Cabanel, Amos Matsiko, Ronaldo JFC do Amaral, Katia Carneiro, Marcos Farina, Fergal J O’Brien, Marcia C El-CheikhSUBMIITED TO BRAZILIAN AGENCIES FOR FUNDING
21 Brazil - Ireland Cooperation: What we can offer Brazilian system for ST&I in Health is strong in basic science and the Government is investing in decreasing the gap between science and applied technologyWe have plenty of academic freedom (plasticity)We have a lot of young people engaged in researchIt is very easy to networkCompetencies in basic science can be identified using different tools (Pubmed, Lattes, Google, keywords and sLattes:
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