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RCSI Tissue Engineering Research Group Institute of Biomedical Sciences- UFRJ Research Initiative Fergal J. O’Brien, PhD Professor of Bioengineering &

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Presentation on theme: "RCSI Tissue Engineering Research Group Institute of Biomedical Sciences- UFRJ Research Initiative Fergal J. O’Brien, PhD Professor of Bioengineering &"— Presentation transcript:

1 RCSI Tissue Engineering Research Group Institute of Biomedical Sciences- UFRJ Research Initiative Fergal J. O’Brien, PhD Professor of Bioengineering & Regenerative Medicine Deputy Director of Research, RCSI Head of Tissue Engineering Research Group Deputy Director, Advanced Materials & BioEngineering Research (AMBER) Centre Chair of Executive Committee, Trinity Centre for Bioengineering Dept. of Anatomy, Royal College of Surgeons in Ireland Trinity Centre for Bioengineering, Trinity College Dublin AMBER, RCSI & TCD @TissueEngDublin

2 Biomimetic ECM-derived biomaterials provide an opportunity to alleviate problems with traditional techniques to replace diseased or damaged organs Transplantation/allografting (lack of donors/donor tissue) Autografting (lack of tissue) Permanent prosthesis (not permanent)

3 Research Focus Biomaterial scaffolds - Collagen-glycosaminoglycan/elastin/fibrin - Collagen-ceramic composites - Chitosan, alginate, fibrin & hyularonic acid hydrogels Cells -Tissue-derived mature cells -Bone marrow-derived mesenchymal stem cells -Neural stem cells (OECs) -Adipose stem cells -Amniotic fluid-derived stem cells -Cardiac stem cells -Limbal stem cells Cell signalling - Bioreactors & Mechanobiology - role of biophysical stimuli in regulating stem cell differentiation and tissue formation - Growth factors (controlled release) - Nucleic acids: pDNA/ siRNA/ microRNAs - Platelet-rich plasma Bone Cartilage Cornea Nerve Respiratory Cardiovascular Applications Angiogenesis +GFs +Genes + Co-cultures + Hypoxia + Flow perfusion +Endochondral Ossification

4 Enterprise Ireland Commercialisation Fund POC PC/2007/331 & CFTD TD/2007/112 Patent: WO 2008/096334 HydroxyColl™ A Viable Alternative to Autogenous Bone

5 ChondroColl™ Hyaline Cartilage Regeneration Enterprise Ireland Commercialisation Fund POC PC/2005/226 & CFTD TD/2009/104 Empty Defect ChondroColl Restoration of hyaline cartilage on articular surface following implantation with ChondroColl

6 June, 2012 Intertrade Ireland SEEDCORN Business Competition `Best Emerging Company’ Regional Winner, Nov. 2011

7 - Cells - Tissue - Genes (non-viral technologies) - Growth factors Platelet-rich plasma Scaffolds for controlled, sustained, transient release Scaffolds as Delivery Systems

8 (A) Controlled release of BMP from a collagen-based scaffold at two different rates using two different types of polymeric microcapsules (B) SEM image of growth factor-containing microparticles embedded in the collagen matrix of the scaffold Collagen Scaffolds as Drug Delivery Systems EU and PCT Patent (Pending): O'Brien FJ, Lopez-Noriega A, Quinlan E, Partap S, Kelly H, Cryan SA. Application number: EP12188511.5 (Priority Date, October 15, 2012) López-Noriega et al. (2014) Advanced Healthcare Materials. 3(6):854-9. Lopez Noriega et al. (2014) Journal of Controlled Release 10;187:158-66 Quinlan et al. (2015) Journal of Controlled Release Jan 28;198C:71-79

9 Gene-activated Scaffolds Tierney et al. (2012) Journal of Controlled Release 158(2):304-11 Tierney et al. (2013) Journal of Controlled Release 165(3):173-82 Tierney et al. (2013) Organogenesis. 9(1). 158-164 Raftery et al. (2013) Molecules 18(5):5611-47. Stem cellNano particle + gene Transfected Cell Cell-based manufacture of therapeutic In Vivo Healing Curtin et al. (2015) Advanced Healthcare Materials. [Epub ahead of print]

10 Clinical Collaborators University of Sydney (Orthopaedics) University of Aachen (Cadiovascular) Lund University Hospital, Sweden (Orthopaedics) Kyoto University (Oral and Maxillofacial Surgery) Beaumont Hospital (Otolaryngology) Cappagh Hospital (Orthopaedics) Temple Street (Cranio/maxillofacial Surgery & ENT) Mater Hospital (Otolaryngology) Royal Victoria Eye & Ear Hospital (Ophthalmology) Prof. David Little Prof. Stefan Jockenhoevel Prof. Magnus Tagil Prof. Kazuhisa Bessho Prof. Michael Walsh Prof. John O’Byrne, Prof. Cathal Moran Mr. Dylan Murray, Mr. Alec Blayney Mr. Stephen Kieran Prof. Conor Murphy, Dr. Billy Power Industrial Collaborators Integra Life Sciences, NJ, USA Marigot Ltd SurgaColl Technologies Dr. Simon Archibald Dr. Denise O’Gorman, Pat Forristal, John Gleeson National Academic Collaborators RCSI: School of Pharmacy Molecular and Cellular Therapeutics TCD: Trinity Centre for Bioengineering (TCBE) Dept. of Zoology CRANN UCD: Dept. of Anatomy DCU: National Insitiute for Cellular Biotechnology Dept. of Mechanical Engineering NUI Maynooth: Institute of Immunology Dept. of Biology NUI Galway: National Centre for Bioengineering Science REMEDI University of Limerick: Mech Eng & MSSI Drs. Sally Ann Cryan, Helena Kelly Prof. Ray Stallings, Caroline Jefferies, Dr. Steve Kerrigan, Dr. Danny Kelly, Dr. Conor Buckley Prof. Veronica Campbell, Dr. Bruce Murphy Dr. Paula Murphy Profs. Jonathan Coleman, Ed Lavelle Valeria Nicolosi, Graham Cross Dr. Tom Flanagan Prof. Martin Clynes, Dr. Finbarr O’Sullivan Dr. Triona Lally, Prof. Bernard Mahon Dr. Shirley O’Dea Prof. Peter McHugh, Dr. Laoise McNamara Prof. Frank Barry, Prof. Tim O’Brien, Dr. Mary Murphy, Dr. David Hoey International Academic Collaborators Colombia University: Dept. of Biomedical Engineering Harvard Medical School: Dept. of Orthopaedic Surgery Georgia Institute of Technology: Institute for Bioengineering Wake Forest University: Institute for Regen Medicine Colarado State University: Biomedical Engineering Imperial College London: Biomaterials & Regen. Medicine University College London: Inst of Orthopaedics University of Adelaide: Bone and Joint Research Laboratory Utrecht University: Dept. of Biomedical Engineering Erasmus University, Rotterdam Johannes Gutenberg Univ, Mainz: Institute of Pathology, Federal University of Rio de Janeiro (UFUJ) Prof. Chris Jacobs Prof. David Mooney, Prof. Conor Walsh Prof. Robert Guldberg Prof. Shay Soker Prof. Seth Donahue Prof. Molly Stevens Prof. Robert Brown Prof. Nick Fazzalari, Dr. Ian Parkinson Prof. Wim Hennink & Prof. Gert Storm Prof. Gerjo van Osch, Drs. Eric Farrell & Y. Bastiaansens Prof. James Kirkpatrick Profs. Marcia Cury, Marcos Farina, Valeria de Mello Coelho

11 Research Program in Bioengineering and Cellular Therapies Federal University of Rio de Janeiro Institute of Biomedical Sciences-IBS Rio de Janeiro- Brazil Valeria de Mello Coelho Associate Professor Bioengineering and Cellular Therapies Research Program Coordinator Laboratory of Immunophysiology, Head Histotechniques Core Facility, Head coelhova@histo.ufrj.br

12 Largest Federal University in Brazil 2nd oldest medical school in Brazil (founded in 1808) Tradition in biomedical research - Institute of Biophysics Carlos Chagas Filho, since 1940s - Institute of Biomedical Sciences, since 1960s Focus on engineering and biomedical sciences (biochemistry, cell and tissue biology, neurobiology and stem cell research) http://www.ufrj.br

13 04 Undergraduate Teaching Programs: Anatomy, Cell biology and Developmental biology, Histology and Pharmacology (Attend all Undergraduate Courses in the biomedical field) 02 Graduate Programs: Morphological Sciences Pharmacology and Medicinal Chemistry 07 Research Programs: Bioengineering and Cellular Therapies, Cell Biology, Cellular Differentiation, Drug development, Glicobiology, Pharmacology and Inflammation, Neurosciences Institute of Biomedical Sciences Federal University of Rio de Janeiro http://www.icb.ufrj.br/

14 Mission: the conduction of scientific research aiming at the development of new technologies for regenerative medicine Bioengineering and Cellular Therapies Research Program 17 ICB INTRAMURAL MEMBERS CLAUDIA BATISTA FELIPE LEITE DE OLIVEIRA HELIO DUTRA JOSE BRITO KATIA ARCANJO KATIA CARNEIRO LEONARDO RODRIGUES ANDRADE MARCIA CURY EL-CHEIKH MARCOS FARINA – DEPUTY COORDINATOR MARIA ISABEL DORIA ROSSI MICHELLE BOTELHO PAULO HENRIQUE R CASTRO PAULO LOUZADA RADOVAN BOROJEVIC TATIANA SAMPAIO VALERIA DE MELLO COELHO - COORDINATOR 10 EXTRAMURAL MEMBERS CHRISTINA TAKYIA- UFRJ- IBCCF PRISCILLA FINOTELLI- UFRJ- FF GLORIA SOARES- UFRJ/COPPE LEANDRA BAPTISTA- UFRJ- XEREM MONICA CALASANS MAIA-UFF ALEXANDRE ROSSI-CBPF ANDRE ROSSI- CBPF MAURO GRANJEIRO – INMETRO LEONARDO BOLDRINI- INMETRO JACQUES WECKERMANN - INMETRO ACHIEVEMENTS IN THE LAST 04 YEARS: MORE THAN 200 PUBLICATIONS 1 BOOK and 12 BOOK CHAPTERS 03 PATENTS 31 PhD concluded 39 Masters concluded 08 post-doctoral concluded 23 INTERNATIONAL EXCHANGE Visits/ Cooperation with France, Stanford University, National Institutes of Heath, USA/ Organization of international meetings BRAZILIAN FINANCIAL SUPPORT:

15 Marcos Farina UFRJ José Mauro Granjeiro UFF/INMETRO Radovan Borojevic UFRJ/INMETRO AMABLE, PAOLA ROMINA ; TEIXEIRA, MARCUS VINICIUS ; CARIAS, ROSANA BIZON ; Granjeiro, José Mauro ; BOROJEVIC, RADOVAN. Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly. Stem cell research & therapy, v. 5, p. 53,2014. ROSSI, AL. ; MOLDOVAN, SIMONA ; Querido, W ; ROSSI, A; WERCKMANN, J; ERSEN, OVIDIU ; FARINA, MARCOS. Effect of strontium ranelate on bone mineral: Analysis of nanoscale compositional changes. Micron (Oxford. 1993), v. 56, p. 29-36, 2014. Alexandre Rossi CBPF Program Overview: Research focus LOPEZ EO, MELLO A, SENDAO H, COSTA LT, ROSSI AL, OSPINA RO, BORGHI FF, SILVA FILHO JG, ROSSI AM. Growth of Crystalline Hydroxyapatite Thin Films at Room Temperature by Tuning the Energy of the RF-Magnetron Sputtering Plasma ACS Appl Mater Interfaces. 2013 Oct 9;5(19):9435-45. Baptista, LS. ; SILVA, KR. ; PEDROSA, CSG. ; AMARAL, R J.F.C. ; BELIZÁRIO, JV ; BOROJEVIC, R ; GRANJEIRO, JOSÉ MAURO. Bioengineered Cartilage in a Scaffold-Free Method by Human Cartilage-Derived Progenitor Cells: A Comparison With Human Adipose-Derived Mesenchymal Stromal Cells. Artificial Organs, v. 37, p. 1068-1075, 2013. Monica Calasans UFF Valiense, HB ; Alves, A.T.N.N. ; Mavropoulus, E ; TANAKA, M. ; ROSSI, A. M. ; BARRETO, M. ; Granjeiro, JM ; CALASANS-MAIA, M. D.. In vitro and in vivo evaluation of strontium-containing nanostructured carbonated hydroxyapatite/sodium alginate for sinus lift in rabbits. Journal of Biomedical Materials Research. Part B, Applied Biomaterials, v. 1, p. 1-9, 2015.

16 BLOISE, F. F. ; OLIVEIRA, F. L. ; NOBREGA, A. F. ; VASCONCELLOS, R. ; CORDEIRO, A. ; PAIVA, L. S. ; TAUB, D. D. ; BOROJEVIC R. ; PAZOS-MOURA, C. C. ; DE MELLO-COELHO, V.. High levels of circulating triiodothyronine induce plasma cell differentiation. Journal of Endocrinology, 2014 TAKIYA, CM ; BOROJEVIC, R. Cardiac and renal cell therapies: similarities and differences. Kidney International Supplements, v. 1, p. 99-102, 2011. TRIGO, FMB. ; LUIZON,MR. ; DUTRA, HÉLIO S. ; MAIOLINO, Â ; NUCCI, M ; SIMÕES, BP. Interaction between IL-6 and TNF-α genotypes associated with bacteremia in multiple myeloma patients submitted to autologous stem cell transplantation (ASCT). Leukemia Research Reports, v. 3, p. 76-78, 2014. BRAND, C. ; OLIVEIRA F. L ; Ricon, L. ; FEMINO, M. L. ; BOLDRINI, L. C. ; Hsu, D. K ; Liu, F.-T. ; CHAMMAS, R. ; BOROJEVIC, R. ; FARINA, M. ; EL-CHEIKH, MC.. The bone marrow compartment is modified in the absence of Galectin-3. Cell and Tissue Research (Print), v. 346, p. 427-437, 2011. ANDRADE, LR. ; LINS, U ; FARINA, M ; KACHAR, B ; THALMANN, R. Immunogold TEM of otoconin 90 and otolin relevance to mineralization of otoconia, and pathogenesis of benign positional vertigo. Hearing Research, v. 292, p. 14-25, 2012. ROSADO DE CASTRO PH, Pimentel-Coelho PM, Gutfilen B, Lopes de Souza SA, de Freitas GR, Mendez- Otero R, Barbosa da Fonseca LM 3.Radiopharmaceutical Stem Cell Tracking for Neurological Diseases. Biomed Res Int. 2014;2014:417091. MENEZES, K; NASCIMENTO, MA ; GONÇALVES, JP ; CRUZ, AS; LOPES, DV ; CURZIO, B; BONAMINO, M; DE MENEZES, JL ; BOROJEVIC, R; ROSSI, MID ; COELHO-SAMPAIO, T. Human Mesenchymal Cells from Adipose Tissue Deposit Laminin and Promote Regeneration of Injured Spinal Cord in Rats. Plos One, v. 9, p. e96020, 2014. Isabel Rossi Helio Dutra Tatiana Sampaio Felipe L Oliveira Christina Takiya Leonardo Andrade Leandra Baptista Priscilla Finotelli Paulo H R Castro Michelle Botelho Katia Carneiro Jose Brito Paulo Lousada Claudia Batista

17 1. BIOMATERIALS FOR TISSUE REPAIR: production and characterization of biomaterials for application in the repair of various tissues such as skin, nerve, bone and cartilage; 2. CHARACTERIZATION OF MINERAL PARTICLES: The chemical, biophysical and morphological characterization of nanoparticles and of crystals obtained upon experimental biomineralization; 3. CONTROL OF CELL PROLIFERATION AND DIFFERENTIATION: aiming at an efficient manipulation of stem or progenitor cells for use in cell therapy; 4. IMMUNOMODULATION STUDIES associated to cell therapy and to natural processes such as aging, degenerative diseases or cancer; 5. THE UNDERSTANDING OF THE ROLE OF TISSUE MICROENVIRONMENTS as a determinant of cell behavior in several pathophysiological processes. Program overview: research focus

18 Month seminars (at UFRJ, InMetro, CBPF, UFF): -Thesis presentation -Intramural and Extramural Researchers -International Researchers. Visiting Researchers Annual Workshop -Presentation of projects under development by Members of the Bioengineering Researcher program -To stimulate collaboration and plan organization of meetings and application to Research grants Annual discipline at the Graduate Program in Morphological Sciences: Bioengineering and Tissue Repair (2 weeks- Lectures and scientific papers presentation) – participation of all Program members and International speakers In 2015 – will be offered to the undergraduate courses of Biophysics, Nanotechnology, and Biomedical Sciences - at UFRJ Program overview: annual activities

19 BRAZILIAN GROUPIRISH GROUP PLATELET-RICH PLASMASCAFFOLDS CELL BIOLOGYCARTILAGE TISSUE ENGINEERING BASIC SCIENCEAPPLIED SCIENCE RONALDO AMARAL PhD student SCIENTIFIC KNOW-HOW Platelet-rich plasma released differently stimulates cellular commitment towards the chondrogenic lineage according to concentration. Ronaldo JFC do Amaral, Amos Matsiko, Marcel RP Tomazette, Wanessa KR Rocha, Eric Cordeiro-Spinetti, Tanya J Levingstone, Marcos Farina, Fergal J O’Brien, Marcia C El-Cheikh, Alex Balduino SUBMITTED AT JOURNAL OF TISSUE ENGINEERING FERGAL O`BRIEN 2014

20 AMOS MATSIKO IRISH GROUPBRAZILIAN GROUP SCAFFOLDSIMMUNOLOGY 3D CELL CULTUREEPIGENETICS APPLIED SCIENCEBASIC SCIENCE SCIENTIFIC KNOW-HOW Myeloid differentiation in collagen-hyaluronic acid scaffolds under epigenetic control Mariana Cabanel, Amos Matsiko, Ronaldo JFC do Amaral, Katia Carneiro, Marcos Farina, Fergal J O’Brien, Marcia C El-Cheikh SUBMIITED TO BRAZILIAN AGENCIES FOR FUNDING MARCIA CURY EL-CHEIKH 2014

21 Brazilian system for ST&I in Health is strong in basic science and the Government is investing in decreasing the gap between science and applied technology We have plenty of academic freedom (plasticity) We have a lot of young people engaged in research It is very easy to network Competencies in basic science can be identified using different tools (Pubmed, Lattes, Google, keywords and e- mails Lattes: http://buscatextual.cnpq.br/buscatextual/busca.do Brazil - Ireland Cooperation: What we can offer

22 OBRIGADA!


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