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IP: IgG AEG-1 IP Input AEG-1 Akt3 AEG-1 Akt3 IB: Supplementary Figure 1.

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Presentation on theme: "IP: IgG AEG-1 IP Input AEG-1 Akt3 AEG-1 Akt3 IB: Supplementary Figure 1."— Presentation transcript:

1 IP: IgG AEG-1 IP Input AEG-1 Akt3 AEG-1 Akt3 IB: Supplementary Figure 1

2 A B Supplementary Figure 2

3 Supplementary Figure 3

4 AEG-1 Myc tag Akt2 Actin p-Akt2-S474 Vector Control Akt2-PH Akt1-PHAEG-1Akt2-PH+AEG1Akt1-PH+ AEG1 Ctrl siRNA Akt2 -siRNA Ctrl -siRNA Akt1-siRNA Akt2 p-Akt2-S474 Akt1 ttAkt Actin p-Akt-S473 AB AEG-1 Akt2 Akt1 Actin AEG-1 — — + — + + Akt2-siRNA — + + — — — Akt1-siRNA — — — + + — AEG-1 Akt2 Akt1 Actin Myc tag AEG-1 — — + — + + Akt2-PH — + + — — — Akt1-PH — — — + + — C D Supplementary Figure 4

5 Supplementary Figure 5 A B

6 0’ 15’ 30’ 1h 2h 3h p-Akt S473 p-Akt T308 ttAkt Actin ControlAEG-1-siRNAAEG-1 OE CtrlAEG-1 OEAEG-1-siRNA S473 1/slope T308 1/slope P values (S/T)0.0038/ / / Linear regression analysis of p-Akt S473 and T308 half-life Supplementary Figure 6 +LY294002: A B

7 Supplementary Figure 7 AEG-1 — — — — Akt2-PH — + — + — + — + CAPE — — + + — — + + AEG-1 p-Akt2-S474 Akt2 p-P65 P65 Actin

8 Supplementary Figure 8

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13 Supplementary Table 1

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25 S-Table 2Source: US BIOMAX GL801Brain tumor TMAScores of AEG1 and Akt staining PositionGenderAgeOrganPathologyGradeTypeAEG1 scoreAkt2 score A1F68BrainAstrocytoma of right temporal lobeIIMalignant43 A2F44BrainGanglioglioma of right frontal lobe-Malignant54 A3F36BrainAstrocytoma of left temporal lobeIIMalignant86 A4M41BrainAstrocytoma of right frontal lobeIMalignant33 A5F14BrainAstrocytomaIIMalignant22 A6M39BrainAstrocytomaIMalignant45 A7M36BrainAstrocytoma of frontal lobeIMalignant32 A8F30BrainAstrocytomaIMalignant22 A9F37BrainAstrocytoma of right temporal lobeIIMalignant22 A10M52BrainAstrocytoma of brainIMalignant33 B1F42BrainAstrocytoma of left occipital parietal lobeIIMalignant45 B2M16BrainAstrocytoma of right occipital lobeIIMalignant55 B3M47BrainGanglioglioma of left temporoparietal lobe-Malignant76 B4M17BrainAstrocytoma of fourth ventricleIIMalignant59 B5M31BrainAstrocytoma of left temporal lobeIIMalignant22 B6F34BrainAstrocytomaIIMalignant32 B7F47BrainAstrocytoma of left parietal lobeIIIMalignant66 B8F34BrainColloid cell hyperplasia of left brain-Malignant43 B9F40BrainAstrocytoma of right temporoparietal lobeIIIMalignant33 B10F40BrainAstrocytoma of frontal lobeIIIMalignant23 C1M53BrainAstrocytoma of foramen magnumIIIMalignant54 C2M58BrainAstrocytoma of left temporal lobeIIMalignant86 C3M33BrainAstrocytomaIIMalignant97 C4M43BrainBrain tissue-Malignant55 C5F48BrainAstrocytoma of right temporal lobeIIMalignant54 C6F30BrainAstrocytoma of fourth ventricleIIMalignant34 C7M53BrainAstrocytoma of right temporal lobeIIMalignant45 C8M65BrainAstrocytomaIIIMalignant44 C9F33BrainAstrocytoma of left temporoparietal lobeIIMalignant55 C10M43BrainAstrocytoma of right cerebral ventricleIIMalignant22 D1M40BrainOligodendroglioma of right frontal lobe-Malignant64 D2M39BrainAstrocytoma of brainIIIMalignant64 D3F36BrainAstrocytoma of brainIIIMalignant810 D4F36BrainAstrocytoma of left temporal lobeIIMalignant53 D5M53BrainAstrocytoma of left temporal lobeIIMalignant63 D6F47BrainAstrocytoma of intracranialIIMalignant43 D7F16BrainAstrocytoma of cerebellumIIIMalignant35 D8M39BrainAstrocytoma of left temporal lobeIIIMalignant33 D9M35BrainAstrocytoma with necrosis of left frontal lobeIIIMalignant43 Supplementary Table 2

26 D10M59BrainAstrocytoma of left temporal lobeIIIMalignant45 E1M48BrainAstrocytoma of temporoparietal lobeIIIMalignant97 E2M36BrainAstrocytoma of right cerebelliIIIMalignant99 E3F51BrainAstrocytomaIIIMalignant85 E4M47BrainAstrocytomaIIIMalignant106 E5M44Brain Brain tissue with colloid cell hyperplasia of right temporal lobe-Malignant87 E6M68BrainAstrocytomaIIIMalignant75 E7F41BrainAstrocytoma of brainIIIMalignant86 E8M60BrainAstrocytoma of left frontal lobeIIIMalignant55 E9M53BrainAstrocytoma of brainIIMalignant44 E10F48BrainAstrocytoma of left frontal lobeIIIMalignant24 F1M35BrainAstrocytoma of left temporal lobeIIMalignant52 F2M40BrainAstrocytoma of left occipitalIIIMalignant85 F3F47BrainAstrocytoma of left temporal lobeIIIMalignant87 F4M9BrainAstrocytoma of right temporal lobeIVMalignant109 F5F48BrainAstrocytoma of left temporal lobeIIIMalignant97 F6M68BrainAstrocytoma of right temporal lobeIIIMalignant98 F7F49BrainAstrocytoma of right temporal lobeIIIMalignant99 F8M5BrainAstrocytoma of fourth ventricleIVMalignant96 F9M76BrainAstrocytomaIIMalignant44 F10F30BrainAstrocytoma of temporal lobeIVMalignant53 G1M45BrainTumor necrosis tissue of right frontal region-Malignant85 G2M53BrainAstrocytoma of left occipital parietal lobeIIIMalignant108 G3M33BrainMalignant oligodendroglioma-Malignant95 G4M17Brain Desmoplastic astrocytoma of left temporoparietal lobe-Malignant97 G5M44BrainAstrocytoma of intracranialIIMalignant65 G6F39BrainEpendymoblastoma of posterior fossa-Malignant109 G7M2BrainEpendymoma of brain parietooccipital-Malignant77 G8M48BrainEpendymoma of brain-Malignant87 G9M72BrainEpendymoma of right parietal lobe-Malignant77 G10F13BrainEpendymoma of intracranial-Malignant67 H1F68BrainMatched cerebrum tissue of No.01-Normal 3 H2F44BrainMatched cerebrum tissue of No.02-Normal33 H3F36BrainMatched cerebrum tissue of No.03-Normal54 H4M41BrainMatched cerebrum tissue of No.04-Normal53 H5F33BrainNormal cerebrum tissue-Normal43 H6F37BrainNormal cerebrum tissue-Normal45 H7M39BrainNormal cerebrum tissue-Normal22 H8M52BrainNormal cerebrum tissue-Normal43 H9F47BrainNormal cerebrum tissue-Normal44 H10M72BrainNormal cerebellum tissue-Normal33

27 Supplementary Figure 9 +LY294002: 0’ 15’ 30’ 1h 2h 3h Control AEG-1 OE AEG-1 KD p-Akt1 S473 Akt1 Actin


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