Presentation on theme: "Viral immune evasion strategies Frank Momburg Division of Molecular Immunology German Cancer Research Center, Heidelberg"— Presentation transcript:
Viral immune evasion strategies Frank Momburg Division of Molecular Immunology German Cancer Research Center, Heidelberg firstname.lastname@example.org
Viral immune evasion strategies 1. Viral evasion of MHC class I-mediated antigen presentation 2. Viral evasion of MHC class II-mediated antigen presentation 3. Viral evasion of natural killer cell activation 4. Viral interference of chemokine/cytokine action 5. Viral evasion of antibody responses and complement attack 6. Viral interference with apoptosis
Life styles and survival strategies of viruses > In most cases: elimination of the virus Small RNA virus e.g. human influenza virus Large DNA virus e.g. CMV Interference with adaptive immunity Replication at immunoprivileged sites Molecular latency > Persistent infection „hit and run“„hit and hide“ Interference with innate immunity, typically type I interferon Blockade of host gene transcription Antigenic hypervariability
Cytomegalovirus genome (235 kbp, > 200 open reading frames) Hepatitis B virus Influenzavirus Adenovirus Vaccinia virus CMV
Antigenic drift and antigenic shift of influenza virus
Acute infection and latent phase (herpes simplex virus)
Presentation of antigenic peptides by MHC class I molecules T cell receptor 1111 2222 3333 VVVV VVVV CCCC CCCC 2m2m2m2m CD8 coreceptor Cytotoxic T cell Antigen presenting cell Antigen presenting cell
Role of MHC class I-mediated antigen presentation in antiviral immune responses The antigen-specific T cell receptors of CD8 + cytotoxic T cells (CTL) recognize MHC class I molecules displayed on the surface of virus-infected cells or on professional antigen presenting cells (dendritic cells, macrophages, B cells). These MHC-I molecules present peptide antigens that are cleaved out of endogenously synthesized viral proteins by proteasomes and other intracellular proteases. Alternatively, viral antigens can be cross-presented by professional APC after uptake of viral particles or of apoptotic, virus-infected cells.
Viral subversion of MHC class I-mediated antigen presentation 1. Interference with the biosynthesis of MHC-I molecules AdenovirusE1ATranscriptional inhibition (via decrease of NF B) of MHC-I heavy chain, TAP1/2, and LMP2/7 expression 2. Interference with antigen processing by proteasomes HCMVpp65Kinase (IE phase), inhibits generation of antigenic peptides from a 72 kDa transcription factor (important antigen in immediate early phase) EBVEBNA-1Contains a 239 residue Gly-Ala repeat that blocks proteasomal processing MuLVenv-p15ESingle amino acid change (K R) in FMR subtypes loss of CTL epitope through altered proteasomal cleavage site
Viral subversion of MHC class I-mediated antigen presentation 2. Interference with antigen processing by proteasomes HCMVpp65Kinase (IE phase), inhibits generation of antigenic peptides of a 72 kDa transcription factor (important antigen in immediate early phase) EBVEBNA-1Contains a 239 residue Gly-Ala repeat that blocks proteasomal processing MuLVenv-p15ESingle amino acid change (K R) in FMR subtypes loss of CTL epitope through altered proteasomal cleavage site
Viral subversion of MHC class I-mediated antigen presentation 3. Interference with peptide transport by TAP HSV1/2ICP47Cytosolic protein blocking peptide binding to TAP HCMVUS6ER protein that inhibits peptide translocation (inducing a conformational change that prevents ATP binding to TAP1) BHV/EHV/PRVUL49.5 Inhibition of peptide translocation (inducing a transport-incompetent arrest and proteasomal degradation of TAP) HIV?Inhibition of TAP
Viral subversion of MHC class I-mediated antigen presentation 4. Interference with the heterotrimeric assembly of MHC-I molecules in the ER HCMVUS2, US11Dislocation of MHC-I through the Sec61p channel to the cytosol proteasomal degradation HIVVpuDislocation from ER to cytosol proteasomal degradation Adenovirus E3/19K Retains MHC-I in the ER, inhibits TAP/tapasin association HCMVUS3Retains MHC-I in the ER, inhibition of tapasin- dependent peptide loading M HV-68mK3Ubiquitination/degradation of TAP/tapasin associated MHC-I HPVE5Retains MHC-I in the Golgi complex
Viral factors interfering with the peptide loading complex and assembly of MHC class I molecules
Retrotranslocation of MHC class I molecules from the ER to the cytosol for proteasomal degradation
Viral subversion of MHC class I-mediated antigen presentation 5. Interference with the intracellular trafficking of MHC-I molecules MCMVm152/gp40Retains MHC-I in cis-Golgi network MCMVm04/gp34Blocks antigen presentation by MHC-I on the cell surface MCMVm06/gp48Targets MHC-I to late endosomes/lysosomes degradation HPVE5Retains MHC-I in the Golgi complex HIVNefEnhanced endocytosis of MHC-I sequestration in trans-Golgi network HHV-8(KSHV) K3, K5Enhanced endocytosis of HLA-A, B, (C, E), ubiquitination/degradation HHV-7U21Diversion of MHC-I molecules to lysosomes degradation
Viral factors obstructing the intracellular trafficking of MHC class I molecules
Viral inhibition of MHC class I-mediated antigen presentation 6. Mutation of CTL epitopes Mutations in class I-binding peptides represent an important mechanism to prevent antiviral CTL responses, leading to loss of MHC-I binding, loss of CTL recognition, or antagonism of an existing CTL response. HIV, EBV, HBV, HCV, Influenza virus
Multiple immune evasion mechanisms employed by HIV
Role of MHC class II-mediated antigen presentation in antiviral immune responses Viruses that enter cells through phagocytosis or receptor- mediated endocytosis, or that are enveloped in the trans-Golgi network/early endosome, can undergo degradation into antigenic peptides through the action of endosomal/lysosomal proteases. Such peptides are loaded onto MHC class II (MHC-II) molecules for presentation to CD4 + T cells. MHC-II molecules are constitutively expressed only by B cells, macrophages, dendritic cells and endothelial cells, however, can be induced on a variety of other cell types by IFN- in the course of inflammtory responses. Activated CD4 + T cells function as helper T cells assisting the maturation of CD8 + CTL and thus coordinate antiviral responses, or may possess CTL activity themselves (in humans).
MHC class II-mediated presentation of exogenous viral antigens
MHC class II-mediated presentation of endogenous viral antigens
Viral evasion of MHC class II-mediated antigen presentation 1. Interference with the biosynthesis of MHC-II molecules MCMVM27IFN-induced transcriptional upregulation of MHC-II expression inhibited (interference with Jak1/STAT pathway CIITA ) AdenovirusE1AIFN-induced transcriptional upregulation of MHC-II expression inhibited
Viral evasion of MHC class II-mediated antigen presentation 2. Interference with the intracellular trafficking of MHC-II molecules HCMVUS2Dislocation of MHC-II to cytosol proteasomal degradation EBVBZLF2Association with HLA-DR(DP, DQ) chains on the cell surface inhibition of antigen presentation BPVE6Interaction with AP-1 adaptor protein disturbs intracellular transport BPV/HPVE5Interaction with 16K subunit of the vacuolar ATPase endosomal acidification and MHC-II processing
Viral interference with MHC class II- mediated antigen presentation
Viral inhibition of MHC class II-mediated antigen presentation 3. Interference with the costimulatory molecule CD4 (T helper cells) HIV-1,-2, SIVNefEndocytosis of CD4 via AP-2 adaptor complex (endocytosis motif in Nef), transport to lysosomes via COPI association HIV-1VpuInduction of CD4 ubiquitination in the ER dislocation to the cytosol proteasomal degradation Myxoma virus?Internalization of CD4 lysosomal degradation T cell receptor MHC-II/peptide 1111 1111 2222 VVVV VVVV CCCC CCCC CD4 coreceptor Helper T cell Antigen presenting cell Antigen presenting cell 2222
Molecular mechanisms employed by the HIV Nef protein
Role of NK cells in antiviral immune responses Natural Killer (NK) cells are important effector components of the innate immune system that function in the initial defense against viruses via direct cellular cytotoxicity and through the production of inflammatory cytokines that promote the influx of CD8 + T cells. For the control of certain viral infections in mice (RSV, MCMV), an early NK-mediated cytotoxicity and IFN- production plays an important role. In humans, a congenital lack of NK cells is associated with severe herpesvirus infections and low NK cell numbers correlate with more rapid progession towards AIDS in HIV-positive patients. NK cells are preferentially activated in the presence of low amounts of MHC-I molecules ("missing self") since particular allelic variants of polymorphic MHC-I molecules trigger inhibitory NK receptors.
Viral evasion of NK cell activation 1. MHC-I homologs binding to inhibitory NK receptors MCMVm144Peptide-free MHC-I homolog, 2 -microglobulin- associated, inhibitory NK receptor unknown MCMVm157Distant MHC-I homolog; binding to Ly49I inhibitory receptor in CMV-susceptible mouse strain, to Ly49H activating receptor in CMV-resistant strain HCMVUL18MHC-I homolog, 2 -microglobulin and peptide- associated, binds to NK-inhibitory LIR-1/ILT-2 receptor
Viral evasion of NK cell lysis 2. Selective expression of NK-inhibitory MHC-I molecules HIV-1Nef No downregulation of NK-inhibitory HLA-C, -E, -G molecules HCMVUS2, US11 No downregulation of NK-inhibitory HLA-C, -E, -G molecules HCMVUL40 Encodes a leader sequence-derived peptide that is loaded onto inhibitory HLA-E molecules in a TAP-independent fashion 3. Downmodulation of costimulatory molecules HHV-8K5Decreased surface expression and ubiquitination of costimulatory (KSHV) ICAM-1 and B7-2 molecules HCVE2Ligation of costimulatory CD81(TAPA-1) tretaspan molecule
TAP-independent NK cell inhibition by HLA-E and the HCMV UL40 protein
Viral evasion of NK cell lysis 4. Inhibition of NK-activating ligands HCMVUL16Intracellular retention of the NK cell activating NKG2D ligands MICB and ULBP1,2 (does not affect MICA and ULBP3) MCMVm152/gp40Inhibits surface expression of NK activating ligand H-60
Role of cytokines and chemokines in antiviral responses Cytokines and chemokines are secreted polypeptides that coordinate inflammation, cellular activation, proliferation, differentiation, and chemotaxis. Cytokines and chemokines are immune mediators that are produced early upon virus infection. They induce and maintain innate as well as adaptive immune responses. Cytokines are responsible for flu-like symptoms such as myalgia, fever, headache and drowsiness which are common manifestations of acute virus infections. Cytokines can be powerful antiviral mediators, allowing clearance of virus infection in the majority of cases. Double-stranded viral RNA type-I interferons (IFN- / ) protein translation , cell proliferation , cellular RNases for viral RNA . Pro-inflammatory cytokines are of particular importance and frequently targeted by viruses: IL-1, IL-12, TNF- , IFN- / , IFN- , and several chemokines that activate leukocyte migration.
Viral evasion of cytokine action 1. Interruption of cytokine production and maturation AdenovirusE1A Blocks IRF-3-induced transcription of IFN- / HPVE6 Blocks IRF-3-induced transcription of IFN- / HCMV? Inhibition of transcription of MCP-1 chemokine Myxoma virusSERP-2 Inhibition of IL-1 converting enzyme (ICE) IL-1 Cowpox virusCrmA/Spi-2 Inhibits several caspases, including ICE Vaccinia virusB13R Inhibits several caspases, including ICE Measles virusHemagglutinin Binding to CD46 (complement receptor/regulator for C3b/C4b) on infected macrophages/DC IL-12 production Th1 response (IFN- production by T and NK cells)
Viral evasion of chemokine/cytokine action 2. Interference with the receipt of the chemokine/cytokine signal A. Viral chemokine receptor homologs HCMVUS28Functional CCR1 chemokine receptor (binds MCP-1, MIP-1 , RANTES), co-receptor for HIV entry HCMVUL33CCR1 chemokine receptor, expressed in viral coat MCMVM33CCR1 homolog, role in salivary gland dissemination and replication HVSECRF3Functional CXCR2 chemokine receptor HHV-8ORF74Constitutively active, agonist-independent CXCR2 receptor HHV-6/7?Downregulation of cellular CXCR4 chemokine receptor in infected CD4 + T cells response to ligand SDF-1 B. Viral chemokine/cytokine homologs HHV-8vMIP-IIBroad spectrum CC-, CXC- and CX3C chemokine antagonist MCVMC-148PBroad spectrum CC- and CXC chemokine antagonist HHV-8vMIP-IMIP-1 homolog, CCR8 agonist, Th2 response HHV-6U83MIP-1 homolog, CC chemokine agonist MCMVm131CC chemokine homolog, promotes virus dissemination HHV-8 vIL-6IL-6 homolog, increases angiogenesis and hematopoesis (role in Kaposi sarkoma, IL-6R + ) EBVvIL-10IL-10 homolog, antagonizes Th1 responses MCVMC-51LSecreted, binds IL-18 NK activation , Th1 response C. Virus-encoded secreted cytokine receptors and chemokine-binding proteins Cowpox virus CrmB, CrmC Secreted TNF-R homologs, sequester TNF- and LT- Myxoma virus MT-2 Secreted TNF-R homolog, sequesters and inhibits TNF- HCMV UL144 TNF-R homolog, retained intracellularly Vaccinia virus B18-R Secreted type I IFN-R homolog, sequesters IFN- Vaccinia virus B8-R Secreted type II IFN-R homolog, sequesters IFN- Myxoma virus M-T7 Secreted type II IFN-R homolog, sequesters IFN- Vaccinia virus B15-R Secreted IL-1 -R homolog, sequesters IL-1 EBV BARF-1 Secreted protein, sequesters CSF-1
Viral evasion of chemokine/cytokine action 3. Interference with interferon-mediated effector functions A. Inhibition of IFN-induced gene transcription Adenovirus E1ADepletion of STAT1 or p48 ISGF3 IFN-induced transcriptional activation HPV E7Sequestering of IRF1 IFN-induced gene transcription HCMV ?Depletion of Jak1 kinase and p48 ISGF3 HHV-8 vIRF K9IRF antagonist, competition with IFN-induced transcription B. Interference with IFN-induced cellular defence mechanisms EBV EBNA2IRF antagonist, competition with IFN-induced transcription HCV E2Inhibits phosphorylation of eIF2 by dsRNA-dependent protein kinase (PKR) HSV 1 34.5Activates protein phosphorylase 1 to dephosphorylate eIF2 Vaccinia virus K3LeIF2 homolog, inhibits PKR Adenovirus VAI RNAViral RNA, inhibits PKR EBV EBER IViral RNA, inhibits PKR Vaccinia virus E3LSequesters ds-RNA, prevents PKR and 2’5’-OS activation HSV 2'-5'-(A) analogInhibits 2'-5'-oligoadenylate synthase RNaseL activity
Viral evasion of antiviral cytokine effector functions
Role of antibodies and complement in antiviral reponses Protective antibodies bind to virus surface structures and can block their interaction with cellular receptors. Antibody-tagged (opsonized) viruses can be cleared from the circulation via IgG-Fc receptors expressed by phagocytes or by germinal center follicular dendritic cells ( viral spread possible). Fc receptors (CD16) instruct NK cells to lyse antibody-coated virus-infected cells by antibody-dependent cellular cytotoxicity (ADCC). IgM- and IgG-coated viruses can be neutralized by the classical complement pathway. Viruses tagged with the complement component C3b (or C4b, C3bi) can be phagocytosed via the CR1 (CD35) complement receptor. Viruses coated with C3b cleavage products (C3d, C3bi) can activate the CR2 (CD21) complement receptor of B cells. Follicular dendritic cells expressing CR1/CR2 complement receptors trap opsonized viruses and stimulate antibody production by germinal center B cells. Phagocytosis of opsonized viruses elicits antigen processing, release of pro-inflammatory cytokines and T cell activation. Byproducts of the complement pathway (C3a, C4a, C5a) function as chemotactic peptides and amplify the inflammatory process.
Viral evasion of antibody responses Viral Fc receptor homologs HSV-1gE-gIFc R homolog for monomeric or aggregated IgG, expressed on virus particles or infected cells, gE contains YXXL internalization motif for endocytosis of immune complexes inhibition of ADCC HSV-2gEFc R homolog MCMVFcr1Fc R homolog PRVgE-gIFc R homolog VZVgE-gIFc R homolog MVNPLigates cellular inhibitory Fc RII receptor (CD32) downregulation of antibody production by B cells
Evasion of antibody responses by viral Fc receptors
Viral interference with apoptosis (programmed cell death) Replicating viruses may stimulate suicide of the host cell directly or provoke recognition by cytolytic T cells and NK cells. These immune effector cells induce apoptosis by secretion of cytotoxic cytokines (such as TNF ) and by processes requiring direct cell-cell contact (perforin/granzyme system or CD95/CD95-L system). Premature cell death would limit the time available for the production of new virions and interrupt cycles of latency and reactivation used by persistent viruses.
Viral evasion of the extrinsic apoptosis pathway 1. Inhibition of TNF/Fas-mediated apoptosis Myxoma virusMT-2 TNF-R homolog, secreted form blocks TNF- induced apoptosis, intracellular form protects T cells from apoptosis Cowpox virusCrmB, C, D Secreted TNF-R homologs, neutralize TNF- and LT- AdenovirusE3-10.4/ Multimeric complex (RID) that forces internalization and 14.5K lysosomal degradation of CD95 (Fas, APO-1) HCMVUL144 TNF-R homolog, retained intracellularly, function unclear 2. Death effector domain (DED)-containing viral proteins (vFLIPs) HHV-8K13 Viral FLICE inhibitory proteins (vFLIPs) with 2 DEDs, bind HVSORF71 FADD/TRADD adaptor proteins, prevent activation of MCVMC159, 160 FLICE (Caspase-8)/Caspase-10 following ligation of CD95, BHV-4BORFE2 TNFR1, TRAMP, TRAIL-R1/R2 death receptors
Viral regulation of the extrinsic apoptosis pathway
Viral evasion of the intrinsic apoptosis pathway 3. Viral caspase inhibitors Cowpox virusCrmA/SPI-2 Serpin, inhibits caspase-8, caspase-1 (ICE), granzyme B Vaccinia virusSPI-2/B13R2Serpin, inhibits caspase-8, caspase-1 (ICE), granzyme B Baculovirusesp35 Inhibits multiple caspases vIAPInhibits initiator caspase-9 and effector caspases-3 and -7 Adenovirus14.7KInhibits caspase-8 4. Viral antiapoptotic Bcl-2 homologs EBVBHRF1Bcl-2 homologs, prevent cytochrom c release from BALF1 mitochondria activation of caspase-9 HHV-8, HVSORF16dito AdenovirusE1B/19Kdito EBVLMP-1Bcl-2 transcription mimics anti-apoptotic TNF-R signals
Viral regulation of the intrinsic apoptosis pathway
Viral immune evasion strategies Co-evolution of viruses with their hosts for millions of years has led to a host immune system of high complexicity and, likewise, sophisticated viral mechanisms to antagonize immunity. Viral antagonists interfere with all relevant effector pathways of the innate and the adaptive immune system including the MHC class I-mediated antigen presentation activating cytotoxic T cells the MHC class II-mediated antigen presentation activating helper T cells the activation of natural killer cells the function chemokines and cytokines orchestrating cellular antiviral responses the function of antiviral antibodies the function of the complement system the induction of the extrinsic and intrinsic pathways of programmed cell death