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EXPERIMENTAL DESIGN  EXTRACTION OF LIMONENE FROM ORANGE PEELS This case of steam distillation is used to isolate the essential oil of limonene from the.

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Presentation on theme: "EXPERIMENTAL DESIGN  EXTRACTION OF LIMONENE FROM ORANGE PEELS This case of steam distillation is used to isolate the essential oil of limonene from the."— Presentation transcript:

1 EXPERIMENTAL DESIGN  EXTRACTION OF LIMONENE FROM ORANGE PEELS This case of steam distillation is used to isolate the essential oil of limonene from the orange peels. Orange peels are cut into pieces, and placed into water. The peel of oranges are boiled in water and the oil produced (limonene) distilled in steam at a temperature just below 100 o c, well below its normal boiling point. The immiscible oil can then be separated.

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4 MODEL SYSTEM: invivo Rats will be injected with carcinogen continuously until a large tumor mass is detected. Rats will be placed on about 10 percent limonene for 21 weeks.  EXPECTED RESULT Limonene will cause about 80 percent inhibition of mammary tumors.

5 Carcinogen in Albino Rats AB

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7 EXPECTED RESULT IN HUMANS The rat metabolism of limonene closely parallels that of humans in terms of both the specific metabolites formed and the time in which they formed. These results suggest that limonene will have similar cancer ‑ treating benefits in humans.

8 limonene inhibits cancer formation by increasing the levels of phase II liver enzymes that bind a wide variety of carcinogens to form less toxic conjugates that are readily eliminated by the digestive and respiratory systems.

9 REFERENCES 1.American Society of Nutrition. “Prevention and Therapy of Cancer by Dietary Monoterpenes.”Journal of Nutrition. N.P Web. 26 Sept Margo Upson. “Causes of Tumors”. Ed. Bronwyn Harris. N.P. 16 July Web 25 Sept Young Life Research Clinic.” Lmonene and Tumor”. Young Living Research. P Sept Web 26 Sept Memorial Sloan-Kettering Cancer Center. “About Herbs, Botanicals & other products”. Integrative Medicine. N.P. Aug Web 28 Sept 2012.


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