Presentation on theme: "EXPERIMENTAL DESIGN EXTRACTION OF LIMONENE FROM ORANGE PEELS"— Presentation transcript:
1EXPERIMENTAL DESIGN EXTRACTION OF LIMONENE FROM ORANGE PEELS This case of steam distillation is used to isolatethe essential oil of limonene from the orangepeels. Orange peels are cut into pieces, andplaced into water. The peel of oranges are boiledin water and the oil produced (limonene) distilledin steam at a temperature just below 100oc, wellbelow its normal boiling point. The immiscible oilcan then be separated.
4MODEL SYSTEM: invivoRats will be injected with carcinogen continuously until a large tumor mass is detected. Rats will be placed on about 10 percent limonene for 21 weeks.EXPECTED RESULTLimonene will cause about 80 percent inhibition of mammary tumors.
7EXPECTED RESULT IN HUMANS The rat metabolism of limonene closely parallels that of humans in terms of both the specific metabolites formed and the time in which they formed. These results suggest that limonene will have similar cancer‑treating benefits in humans.
8limonene inhibits cancer formation by increasing the levels of phase II liver enzymes that bind a wide variety of carcinogens to form less toxic conjugates that are readily eliminated by the digestive and respiratory systems.
9REFERENCES 1. American Society of Nutrition REFERENCES 1.American Society of Nutrition. “Prevention and Therapy of Cancer by Dietary Monoterpenes.”Journal of Nutrition. N.P Web.<http://www.jn.nutrition.org/content/129/3/775S.full> 26 Sept 2.Margo Upson. “Causes of Tumors”. Ed. Bronwyn Harris. N.P. 16 July Web <http://www.wisegeek.org/what-causes-tumors.htm> 25 Sept Young Life Research Clinic.” Lmonene and Tumor”. Young Living Research. P Sept Web < 26 Sept Memorial Sloan-Kettering Cancer Center. “About Herbs, Botanicals & other products”. Integrative Medicine. N.P Aug Web < 28 Sept 2012.