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Single Molecule Biophysics Reading: van Holde Chapter 16 Homework: due Wednesday, April 9 (in class) Van Holde: 16.1, 16.2, 16.3, 16.4 (consult original.

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Presentation on theme: "Single Molecule Biophysics Reading: van Holde Chapter 16 Homework: due Wednesday, April 9 (in class) Van Holde: 16.1, 16.2, 16.3, 16.4 (consult original."— Presentation transcript:

1 Single Molecule Biophysics Reading: van Holde Chapter 16 Homework: due Wednesday, April 9 (in class) Van Holde: 16.1, 16.2, 16.3, 16.4 (consult original papers) Graduate students: Please schedule presentations Remember: Project papers are due last day of class (April 30) Overview: 1.Why single-molecule biophysics? 2.Single-molecule fluorescence, TIRF illumination & applications 3.Atomic Force Microscopy (AFM) & applications (AFM imaging, AFM single- molecule force measurements) 4.Optical tweezers (laser tweezers, laser traps) & applications

2 Single molecule biophysics – Why? Most previous studies have been on the average, ensemble behavior of molecules. Why study single molecules? How any one molecule behaves is not revealed in bulk studies.  An individual enzyme may exist in two or more states of activity that are not revealed by bulk studies. (e.g speed of RNA polymerase transcription).  Analogy: How insurance actuary and novelist look at human behavior.  Static heterogeneity: Different enzyme molecules function at different rates (e.g. a lame population and a fast population)  Dynamic heterogeneity: A given single enzyme can switch between different rates. Can take force measurements (force spectroscopy) on single molecules (previously impossible). E.g. motor proteins, unfolding-refolding proteins Observation and physical manipulation of single, dynamic biomolecules. A recently emerged, new field offering much deeper analysis of molecular behavior. Quick example: The graph shows the speed of a population of polymerase molecules. What is the approximate, ensemble-average speed (as measured by bulk experiments)? What additional information can you obtain from single molecules?

3 Single molecule fluorescence Like Chapter 11 (bulk fluorescence), except on a single fluorophores Challenges: –Photobleaching: Most fluorophores photobleach, i.e. after absorbing many photons ( usually a few million; one photon is one absorption/emission cycle), they chemically rearrange and stop fluorescing (end of experiment). –Signal to noise ratio (need to reduce noise and have sensitive detection). –Must have very, very clean sample. –Detecting weak signals requires very sensitive instrumentation. Needed: –A strong fluorophore (high absorbance and Q-yield). –Intense light source (often laser). –Very sensitive camera/detector –Eliminate all background light. –Illuminate a very small region only Confocal microscopy (images only slices (stacks) of a sample) Use TIR (total internal reflection).(On white board): From Snell’s law : When light encounters a lower index of refraction medium the light gets totally reflected when the incident angle is larger than the critical angle.  We get an evanescent wave (evanescent = tending to vanish like vapor).

4 Prism-based TIR (total internal reflection) illumination n 1 …index of refraction of glass slide n 2 …index of refraction of water  … incident angle … wavelength of light TIRFNo TIRF Relative light intensity 0.125 0.25 0.5 Only molecules close to surface will fluoresce Distance above cover slip 44 nm 88 nm 132 nm Laser Prism and cover slip Microscope objective No signal (background) from molecules far from surface.  TIRF is a technique to only excite fluorophoses on/close to surface  Background noise is strongly reduced L. Peng et al. Microscopy Research & Technique (2007) 70, 372-81

5 Objective-based TIRF illumination Total reflection 1: Objective, 2: Immersion oil n = 1.518, 3: Cover slip n = 1.518, 4: Evanescent field, 5: Mountant n = 1.33…1.38 www.zeiss.com Ray paths (schematic): Angle of incidence smaller than the critical angle. See applet and info at: http://www.olympusmicro.com/primer/techniques/fluorescence/tirf/tirfhome.html 5

6 Application: Single molecule FRET G. Bokinsky et al. “Single-molecule transition-state analysis of RNA folding” (2003) PNAS 100: 9302 SA The hairpin ribozyme:The docking and undocking conformations and a single molecule FRET trace:

7 Application: Single molecule FRET G. Bokinsky et al. “Single-molecule transition-state analysis of RNA folding” (2003) PNAS 100: 9302 2.Rate constants can be extracted from dwell times!! 3.They found a single rate constant for docking k dock = 0.018 sec -1. 4.They found four different rate constants for undocking (k u = 0.01, 0.1, 0.8, 6 sec -1 ), which would have been hard to find in bulk studies). On-time, docked time (msec) Occurrence From figure on previous page: The ribozyme exhibits fluctuations between two states, docked and undocked  FRET signal changes abruptly when a transition occurs. For single rate. 1/e = 37% max 

8 Schematic of an AFM Sample Laser Photodetector Cantilever Force controlled by feedback Substrate Piezo- electric transducer Images from NT-MDT web page

9 Atomic Force Microscopy Advantages: Can achieve atomic resolution on hard, crystalline surfaces. Can often achieve nanometer resolution on biological samples. Imaging can be done in buffer  can image (biological) processes. Can also be used to mechanically manipulate molecules (more in a bit). Gold surface (atomic resolution) S. Dutta, M. J. Snyder, D. Rosile, K. L. Binz, E. H. Roll, J. Suryadi, U. Bierbach, M. Guthold “PT-ACRAMTU, A Platinum–Acridine Anticancer Agent, Lengthens and Aggregates, but does not Stiffen or Soften DNA”, Cell Biochemistry and Biophysics (2013), DOI 10.1007/s12013-013-9614-8 (11 pages)

10 Contact mode AFM Tapping mode AFM scan On Off On Off On Off Tapping 0 nm 4 nm 2  m scan Contact mode (constant force mode): Use cantilever deflection as feedback signal, (use softer cantilevers, can still have lateral forces pushing molecules around) Tapping mode: oscillated cantilever at its resonance frequency (10 kHz to 300 kHz), use cantilever amplitude or phase as feedback signal. (Lateral forces mostly eliminated).

11 A T = 0 s B T = 80 s C T = 130 s 0 nm 5.0 nm E T = 210 s F T = 250 s G T = 290 s D T = 170 s Transcribing RNA Polymerase Imaged by AFM 1,2 100 nm 1. Kasas et al. (1997) Biochemistry 36(3), 461-468 2. Guthold et al. (1999) Biophys. J. 77, 2284-2294 H Kasas moviemovie

12 Normal force measurements. Example 1. Protein unfolding (a) The principal AFM components. (b) Mechanical unfolding of repeating immunoglobulin-like domains (1). As the distance between the surface and tip increases (from state 1 to state 2), the molecule extends and generates a restoring force that bends the cantilever. When a domain unfolds (state 3), the free length of the protein increases, relaxing the force on the cantilever. Further extension again results in a restoring force (state 4). The last peak represents the final extension of the unfolded molecule before detachment from the SFM tip (state 5). (1) Carrion-Vazquez et al. “Mechanical and chemical unfolding of a single protein: a comparison” (1999) PNAS 96 3694-99 Figure from Bustamante, Macosko, Wuite “Grabbing the cat by the tail: Manipulating molecules one by one. Nature reviews Molecular Cell Biology 1 131-6 Force: F = k·  x;k … spring constant of cantilever  x … deflection of cantilever

13 Normal force measurements. Example 2. Ligand binding forces and how they related to the k off rate (force spectroscopy). Protein-ligand is spanned between the tip and the substrate. The tip is then retracted, and, thus, applying a force to the bonds under investigation. If the force is measured as a function of the pulling rate, it is termed force spectroscopy. Figure from: Guthold, M., Superfine, R., Taylor, R. (2001). The rules are changing: Force measurements on single molecules and how they relate to bulk kinetics. Biomedical Microdevices, 3, 9-18

14 Connection between rupture force and off-rate k -1 Assume a two-state model for the reaction. Bell model: an applied force lowers the activation energy. Dissociation rate without an applied force: Dissociation rate with applied force: G. Bell (1978) Science 200, 616-627; E. Evans & K Ritchie (1997) Biophys. J. 72, 1541-55

15 Connection between rupture force and off-rate, k -1 F … rupture force T … temperature k -1 … off-rate x -1 … width of potential k B …Boltzmann constant The rupture force is related to the off-rate Experiment: Measure rupture force as a function of pulling rate. (here done with two different proteins). For this treatment, we assume the reaction proceeds far from equlibrium. The faster you pull the higher the rupture force. Data from F. Schwesinger et al. (2000) PNAS 97, 9972-77, First done by Rief at al. Science (1997) 276, 1109-12

16 How does a laser trap work? Light “consists of photons, which carry momentum. Momentum is conserved.  When light is absorbed, reflected or refracted, tiny forces on the order of piconewtons are generated. For a laser trap we need a light gradient (light is focused). Opposing scattering (down) and gradient forces (up), trap a bead in the focus. If moved from the focus, bead is pulled back toward the focus.  Particle radius has to be larger than wavelength of light (Mie scattering regime).  Need transparent dielectric bead with index of refraction larger than surrounding medium. F scatt. F grad Laser beam lens Bead is below center of focus  force on bead toward focus

17 A trap exerts a linear restoring force proportional to trap stiffness (force is linear to displacement). Using optical tweezers, one can apply pico-newton sized loads and measure nanometer level displacements. Restoring force of a laser trap From Hubmayr lab, Mayo Clinic): http://mayoresearch.mayo.edu/mayo/research/hubmayr/ Ray-diagram for a bead to the left and higher than laser focus

18 Laser tweezers Force clamp: Force on molecule is kept constant by always having bead at the same position in laser trap (  feedback loop moving bead or stage). Position clamp: Position of molecule is kept constant  bead is pulled out of trap and, thus, force increases.

19 Applications of laser tweezers 1.Transcription by single RNA polymerase. Set-up for measuring force-velocity relation of a transcribing RNA polymerase (Wang et al. (1998) Science 282, 902-7. Stall force is about 20 pN.

20 From Block lab: http://www.stanford.edu/group/blocklab/RNAP.html Applications of laser tweezers 2.Transcription by single RNA polymerase. Individual RNA polymerase molecules switch between a fast and a slow mode (Davenport et al. (2000) Science 287, 2497-500 Dumbbell set-up used for some experiments

21 Mechanical properties of DNA Stretching of double-stranded - phage DNA; Length for B DNA ~ 16  m). 1.Up to a length of about 15  m: worm-like chain is straightened (entropy), little force needed. 2.Steep part of curve corresponds to elastic stretching of extended chain. 3.At ~ 17  m a major conformational change occurs  conversion to S-DNA (stretched-DNA). 4.Then DNA denatures and becomes single-stranded. From Smith et al. (1996) Science 271, 795

22 A few more applications Force-producing properties of various molecular motors, including kinesin moving along microtubules 13, actomyosin complexes 28, RNA polymerase 36, 48 and DNA polymerase 11 (Fig. 4b). Mechanical properties of DNA 47132836481147 Mechanical unfolding of proteins 52, 535253 Unfolding of RNA molecules (54) For a somewhat recent review see: Bustamante et al. “Grabbing the cat by the tail: Manipulating molecules one by one.” (2000) Nature reviews Molecular Cell Biology 1 131-6

23 11. Wuite, G. J., Smith, S. B., Young, M., Keller, D. & Bustamante, C. Single-molecule studies of the effect of template tension on T7 DNA polymerase activity. Nature 404, 103-106 (2000). | Article | PubMed | ISI | ChemPort |ArticlePubMedISIChemPort 13. Svoboda, K., Schmidt, C. F., Schnapp, B. J. & Block, S. M. Direct observation of kinesin stepping by optical trapping interferometry. Nature 365, 721-727 (1993). | Article | PubMed | ISI | ChemPort |ArticlePubMedISIChemPort 28. Essevaz-Roulet, B., Bockelmann, U. & Heslot, F. Mechanical separation of the complementary strands of DNA. Proc. Natl Acad. Sci USA 94, 11935-11940 (1997). | Article | PubMed | ChemPort |ArticlePubMedChemPort 47. Smith, S. B., Cui, Y. & Bustamante, C. Overstretching B-DNA: the elastic response of individual double-stranded and single-stranded DNA molecules. Science 271, 795-799 (1996). | PubMed | ISI | ChemPort |PubMedISIChemPort 48. Yin, H. et al. Transcription against an applied force. Science 270, 1653-1657 (1995). | PubMed | ISI | ChemPort |PubMedISIChemPort 52. Kellermayer, M. S., Smith, S. B., Granzier, H. L. & Bustamante, C. Folding-unfolding transitions in single titin molecules characterized with laser tweezers. Science 276, 1112-1116 (1997); erratum 277, 1117 (1997). | PubMed | ISI | ChemPort |PubMedISIChemPort 53.Tskhovrebova, L., Trinick, J., Sleep, J. A. & Simmons, R. M. Elasticity and unfolding of single molecules of the giant muscle protein titin. Nature 387, 308-312 (1997). | Article | PubMed | ISI | ChemPort |ArticlePubMedISIChemPort 54.(J. Liphardt et al. “Reversible Unfolding of Single RNA Molecules by Mechanical Force” (2001) Science 292, 733-737


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