Presentation on theme: "Valvular Interstitial Cell Calcification Liwen Gu July 5, 2005 Prof C. Simmons."— Presentation transcript:
Valvular Interstitial Cell Calcification Liwen Gu July 5, 2005 Prof C. Simmons
Background Endothelial Cells Interstitial Cells http://www.ctsnet.org/edmunds/Chapter28section2.html http://www.ctsnet.org/edmunds/Chapter28section2.html (Fig 28-4) 20 000 deaths caused by valvular diseases in 2002 (US) Aortic sclerosis: thickening of the valve leaftlets calcification Calcification process The American Journal of Cardiology Vol.94; 2004
Objectives How calcification occurs in valvular interstitial cells (VIC) Effects of endothelial cell (EC) paracrine factors on interstitial cells - signaling process Short-term/immediate goal: induce calcification in VIC culture
1 st Experiment Purpose: to induce calcification in VIC culture by providing an osteogenic supplemented (OS) media Method: - Porcine Aortic Valvular Interstitial Cells (PAVIC) – 14 days -added 10 mmol/L βGP to VIC media Hypothesis: -more nodule formation compared to control -alkaline phosphatase activity -mineralization Ann Thorac Surg 2003; 75:457-66
1 st Experiment (cont’d) Results: +OS control (-OS) Explanations: - βGP is not enough -ALP protocol-no positive control
2 nd Experiment Method: -3 time points: 0, 14, 21 days -10 mmol/L βGP + 50μg/mL ascorbic acid + 50ng RANKL (J.J. Kaden et al. Journal of Molecular and Cellular Cardiology 36(2004)57-66)
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