Presentation on theme: "Mechanisms of transcription"— Presentation transcript:
1 Mechanisms of transcription Welcome to Chapter 12Mechanisms of transcription
2 IntroductionUp to this point we have been considering maintenance to the genome ,that is ,how the genetic material is organized ,protected, and replicated.In the next parts ,we will describe the basic processed responsible for gene expression.First let us go into the world of transcription
3 Transcription Vs Replication Transcription is chemically and enzymatically, very similar to DNA replication.Both involve enzymes that synthesize a new strand of nucleic acid complementary to DNA template strand.Moreover ,there are many differences between them.
4 The differences go as follows: RNA is made of ribonucleotidesRNA polymerase ,which catalyzes the reaction,needs no primerThe newly synthesized RNA does not remain base-paired to the template DNA strandLess accurate ,one mistake occurs in 10,000Because of different purpose ,transcription selectively copies only certain parts of the genome and makes anything from one to several hundred,or even thousand.
5 Question :why transcription is less accurate than replication? I think the difference makes good sense if we associate it with the results of the mistakes.DNA is the molecule in which the genetic material is stored,and DNA replication si the process by which that genetic material is passed on.Any mistake can easily be catastrophic:it becomes permanent in the genome of that individual and also gets passed on to subsequent generations.
6 Transcription ,in contrast,produces only transient copies and normally several from each transcribed region.Thus ,a mistake during transcription will rarely do more harm than render one out of many transient transcripts defective.
7 Outline 1. RNA polymerase & Transcription cycle 2. The transcription cycle in bacteria3.Transcription in eukaryotes
8 RNA polymerase & The transcription cycle Topic 1:RNA polymerase & The transcription cycle
9 RNA polymeraseRNA pol come in different forms ,but share many features,especially in those parts of the enzyme directly involved with catalyzing the synthesis of RNARNA pol performs essentially the same reaction in all cells,from bacteria to humans.
10 1 The structure of RNA pol From bacteria to mammals ,the cellular RNA polymerase are made up of multiple subunits .Bacteria have only a single RNA pol ,which is the core enzyme capable of synthesizing RNAEukaryotic cells have three, namely RNA pol I ,II ,and III .They are responsible for synthesis of different kinds of RNA
12 “Crab claw” shape of RNAP (The shape of DNA pol is__) Active center cleft
13 The same color indicate the homologous of the two enzymes a w prokaryoticb’Fig RNAP ComparisonabThe same color indicate the homologous of the two enzymesaweukaryoticRPB2RPB3RPB1RPB11RPB6
14 RNA pol II is the focus ,which is responsible for transcribing most genes-indeed,essentially all protein-encoding genes.RNA Pol I transcribes the large ribosomal RNA precursor gene.RNA Pol III transcribes tRNA genes,some small nuclear RNA genes,and the 5S rRNA gene
15 Since the structure of RNA Pol is this,there come the question:How do they function? Or how do they realize the process of transcription?
16 Transcription by RNA Pol proceeds in a series of steps InitiationElongationTerminationLet us go deep into the details
17 Process 1: Initiation(1)Promoter :the DNA sequence that initially binds the RNA pol(2)Promoter-polymerase complex undergoes structural changes(3)The DNA around the point where transcription unwinds,forming a “bubble”( similar to DNA replication)(4)Again like DNA replication,the direction of transcription is from 5’ to 3’
18 Additionally ,unlike replication,only one of the DNA strands acts as a template on which the RNA strand is built.
19 Transcription Initiation Invoves 3 Defined Steps Form closed complexForm open complexForm stable ternaty complex
21 Closed complex Initial binding of pol to a promoter In this form ,DNA remains double-stranded,and the enzyme is bound to one face of the helix.
22 Open complex DNA strands separate around the transcription site The transcription bubble forms
23 Stable ternary complex Enzyme escape the promoter once it gets further than the 10 bpStable ternary complex contains enzyme,DNA and RNAThen the elongation phase comes
24 Process 2 : ElongationBegins when the enzyme has synthesized a short stretch of RNA (about 10 bp)The RNA pol undergoes further comformational changes to grip the template more firmlyThe enzyme functions:RNA synthesis ,unwind the DNA chains in front,re-anneal it behind,dissociate the growing RNA chain from the template
26 Process 3: TerminationOnce the length of the gene has been transcribed ,the RNA pol must stop and release the productIn some cells ,there are specific,well-characterized sequences.In other cells,it remains to be seen what instructs the termination
28 2-1 Bacterial promoters vary in strength & sequence,but have certain defining features The bacterial core RNA pol can ,in principle ,initiate transcription at any point on a DNA molecule .In cells,polymerase initiates transcription only at promoters.It is the addition of initiation factor called σthat converts core enzyme into the form that initiates only at promoters.That form of the enzyme is called holoenzyme ,which is made up of core enzyme and σfactor
29 Fig 12-5a: bacterial promoter The distance is conserveds70 promoters contain recognizable –35 and –10 regions, but the sequences are not identical.Comparison of many different promoters derives the consensus sequences reflecting preferred –10 and –35 regions
30 The details of σ factorStructure : composed of 4 regions called σregion 1 through σregion 4Function :recognize the site of promoter, mediates binding of polymerase to the promoter
31 Fig 12-6: regions of sRegion 4 recognizes -35 element Region 2 recognizes -10 elementRegion 3 recognizes the extended –10 element
32 Holoenzyme= factor + core enzyme Figure 12-4 In cell, RNA polymerase initiates transcription only at promoters. Who confers the polymerase binding specificity?,
33 Take E.coli as a exampleIn the case of E.coli ,the predominant σfactor is calledσ70 factor .Promoters recognized by σ70 factor share the following characteristic structure:two conserved sequences,each of six nucleotides,are separated by a nonspecific stretch of 17-19nucleotides.The two defined sequences are centered,respectively,at about 10 bp and at about 35 bp upstream of the site where RNA synthesis starts.The sequences are thus called the –35 and –10 regions,or elements.Position +1is the transcription start site.
34 Consensus sequenceAlthough the vast majority of σ70 promoters contain recognizable –35 and –10 regions,the sequences are not identical.Comparison of many different sequences reflecting preferred –10 and –35 regionsPromoters with sequences closer to the consensus are generally “stronger” than those that match less well.By the strength of a promoter,we mean how many transcripts it initiates in a given time.
35 Consensus sequence of the -35 and -10 region BOX 12-1 Figure 1
36 Up-elementAn additional DNA element that binds RNA polymerase is found in some strong promotersUp-element can increases polymerase binding by providing an additional specific interaction between the enzyme and DNAThe magnificence is this : another class of σ70 –promoters lacks a –35region and instead gas a so called “extended-10” element,which compensates for the absence of –35 region.
37 UP-element is recognized by a carboxyl terminal domain of the a-subunit (aCTD), but not by s factor Fig 12-7 s and a subunits recruit RNA pol core enzyme to the promoter
38 Fig 12-5c bacterial promoter Another class of s70 promoter lacks a –35 region and has an “extended –10 element” compensating for the absence of –35 region
39 2-2 The features of transcription in bacteria 1.Transition to the open complex involves structural changes in RNA pol and in the promoter DNA (melting , isomerization, the active center cleft)2.Transcription is initiated by RNA pol without the need for a primer3.RNA pol synthesizes several short RNAs before entering the elongation phase. (Abortive initiation)
40 4.The elongating pol is a processive machine that synthesizes and proofreads RNA.(pyrophosphorolytic editing & hydrolytic editing)5.transcription is terminated by signals within the TNA sequence (Rho-independent Vs Rho-dependent, intrinsic terminators.)
41 Rho-independent terminator contains a short inverted repeat (~20 bp) and a stretch of ~8 A:T base pairs.Fig 12-9
42 Fig 12-11 the r transcription terminator RNA tread trough the “ring”Hexamer,Open ring
44 Transcription in bacteria Vs in eukaryotes Eukaruotes have three different pol (I,II,III), whereas bacteria have only one.Bacteria require only one additional initiation factor(σfactor ) , but several initiation factors are required for efficient and promoter-specific initiation in eukaryotes,which is called the general transcription factors(GTFs)
45 The factors needed for transcription in vivo GTFsPolymeraseMediator complexDNA-binding regulatory proteinsChromatin-modifying enzymes
46 However ,in vitro, the general transcription factors are all that is required,together with pol II . One reason for the difference is that the DNA template in vivo is packaged into nucleosomes and chromatin .This condition complicates binding to the promoter of pol and its associated factors.
47 Core promoterCore promoter refers to the minimal set of sequence elements required for accurate transcription initiation by the pol II machinery.A core promoter is typically about 40 nucleotides long, extending either upstream or downstream of the transcription start site.
48 Fig 12-12: Pol II core promoter TFIIB recognition element (BRE)The TATA element/boxInitiator (Inr)The downstream promoter element (DPE)
49 Fore elements in core promoter BRE : the TFIIB recognition elementThe TATA elementInr : the initiatorDPE: the downstream promoterGenerally , a promoter includes only two or three of these four elements .
50 Regulatory sequencesBeyond the core promoter, there are other sequence elements required for efficient transcription in vivo.These elements constitute the regulatory sequences.They can be grouped into varous categories, reflecting their location, and the organism in question ,as much as their function
51 The regulatory sequences include Promoter proximal elementsUpstream activator sequences (UASs)EnhancersA series of repressing elements called silencers,boundary elements ,insulators .All of them bind regulatory elements ,which help or hinder transcription .
52 Details of GTFsThey can help pol bind to the promoter and melt the DNA.Also help pol escape from the promoter and embark on the elongation phase.Pre-initiation complex = GTFs + promoter ,can initiate the transcription .
53 Formation of pre-initiation complex TFIID recognizes the TATA elementTBP formed when TFIID binds to the TATA elementAnother subunits on this complex are called TAFs ,for TBP associated factors .Other GTFs involved are TFIIA ,B, F,E, H
54 Something about TBPTBP binds to and distorts DNA using a βsheet inserted into the minor groove ,while typically proteins recognize DNA using αhelices inserted into the major groove of DNA.The reason for TBP’s unorthodox recognition mechanism is linked to the need for that protein to distort the local DNA structure.
55 The transcription in eukaryotes TBP binds to and distorts DNA using a b sheet inserted into the minor grooveUnusual (P367 for the detailed mechanism)The need for that protein to distort the local DNA structure
56 The transcription in eukaryotes TBP binds to and distorts DNA using a b sheet inserted into the minor grooveUnusual (P367 for the detailed mechanism)The need for that protein to distort the local DNA structure
57 The other GTFs also have specific roles in initiation 1.TAFsTwo of them bind DNA elements at the promoter;several of them have structural homology to histone proteins :Another appears to regulate the binding of TBP to DNA ,using an inhibitory2.TFIIBThis protein ,a single polypeptide chain,enter the pre-initiation complex after TBP
58 3.TFIIEIt has two subunits ,associating with pol II and recruited to the promoter together with that enzyme.4.TFIIE&TFIIHTFIIE,which ,like TFIIF, consists of two subunits ,binds next and has roles in the recruitment and regulation of TFIIH,which controls the ATP-dependent transition of the pro-initiation complex to the open complex
59 The C-terminal Domain The contraction is CTD In the shape of tail Containing a series of the heptapeptide sequence.Involved in the abortive initiation , promoter escape.Control later steps involving processing of the RNA
60 Mediator complexConsists of many subunits (more than 20), some conserved from yeast to human .There are 7 subunits of 20 ones showing sequence homology between the two organisms.Few of them have any identified function.Only one is essential for transcription of essentially all pol II genes in vivo.
61 Fig 12-17 comparison of the yeast and human mediators
62 Fig assembly of the pre-initiation complex in presence of mediator, nucleosome modifiers and remodelers, and transcriptional activators
63 RNA Pol II holoenzyme ?The dissociation arises the question that whether the RNA Pol II holoenzyme existsThe enzyme is a complex consisting of pol II,Mediator, and some of the GTFsSometimes ,the complex can be isolated from cells as a single one in the absence of DNA
64 Elongation factorsA new set of factors stimulate pol II elongation and RNA proofreading.(1)CTDThe phosphorylation of the CTD leads to an exchange of initiation factors for those factors required for elongation and RNA processing.
65 Beside CDT,various proteins are thought to stimulate elongation by pol II : The kinase P-TEFb :recruited to polymerase by transcriptional activators.TAT-SF1 :recruited by P-TEFbTEIIS : does not affect initiation , but stimulates elongation; contributes to proofreading by pol .
66 RNA processingElongating pol is associated with a new set of protein factors required for various types for RNA processingOnce transcribed, eukaryotic RNA has to be processed in various ways before being exported from the nucleus where it can be translated.In fact ,elongation , termination of transcription,and RNA processing are interconnected-presumably to ensure their proper coordination
67 Fig 12-18 RNA processing enzymes are recruited by the tail of polymerase
68 The processing events include : Splicing :the most complicatedCapping of the 5’ end of RNA :the first RNA processing event ,involving the addition of a modified guanine base to the 5’ end of the RNA .Polyadenylation of the 3’ end of the RNA :mediated by poly-A polymerase .
69 RNA processing 1 5’ end capping The “cap”: a methylated guanine joined to the RNA transcript by a 5’-5’ linkageThe linkage contains 3 phosphates3 sequential enzymatic reactionsOccurs early
70 RNA processing 1 5’ end capping The “cap”: a methylated guanine joined to the RNA transcript by a 5’-5’ linkageThe linkage contains 3 phosphates3 sequential enzymatic reactionsOccurs early
71 Splicing: joining the protein coding sequences Dephosphorylation of Ser5 within the CTD tail leads to dissociation of capping machineryFurther phosphorylation of Ser2 recruits the splicing machinery
72 Fig 12-20 polyadenylation and termination 1. CPSF (cleavage and polyadenylation specificity factor) & CstF (cleavage stimulation factor) bind to the poly-A signal, leading to the RNA cleavage2. Poly-A polymerase (PAP) adds ~ 200 As at the 3’ end of the RNA, using ATP as a substrateFig polyadenylation and termination
73 RNA Pol I&RNA Pol IIIRNA Pol I and III recognize distinct promoters ,using distinct sets of transcription factors ,but still require TBPDifferent from RNA Pol II, they transcribe distinct genes encoding specialized RNAs ,rather than proteins.
74 RNA Pol IRequred for the expression of only one gene ,that encoding the rRNA precursor .The gene transcribed by RNA Pol I is expressed at a extremely high level.Comprises of two parts : the core element & the UCEInitiates with existence of SL1&UBF
75 Pol I promoter recognition Upstream control elementUBF binds to the upstream of UCE, bring SL1 to the downstream part of UCE. SL1 in turn recruits RNAP I to the core promoter for transcriptionFig Pol I promoter region
76 RNA Pol IIIPol III promoters come in various forms,and the vast majority have the unusual feature of being located downstream of the transcription start site.The factors required for transcription are called TFIIIB and TFIIIC ,and those plus TFIIIA for the 5S rRNA gene.
77 Pol III promoter recognition 1. Different forms, 2 Pol III promoter recognition 1. Different forms, 2. locates downstream of the transcription siteTFIIIC binds to the promoter, recruiting TFIIIB, which in turn recruits RNAP IIIFig Pol III core promoter
78 SUMMARYRNA polymerase : crab claw structure & function (mediated the transcription)Transcription Vs replicationTranscription cycle :initiation ,elongation, and terminationTranscription in bacteria (σfactor )Transcription in eukaryotes (RNA pol I , II ,III ;GTFs , core promoters ;regulatory sequence )