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Photobiological Hydrogen Production Using Bioengineered Algae.

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Presentation on theme: "Photobiological Hydrogen Production Using Bioengineered Algae."— Presentation transcript:

1 Photobiological Hydrogen Production Using Bioengineered Algae

2 Outline

3 Why Hydrogen? Highest energy/mass density of all known fuel types Produced Domestically Sustainable (if produced from water) Environmentally Friendly Fuel Cell: No pollutants or greenhouse gases ICE: only (NOx)

4 Chlamydomonas reinhardtii Single-celled green Algae 1939: German researcher Hans Gaffron discovered hydrogen metabolism Hydrogenase Catalyzed reaction, active only in absence of oxygen

5 Low light conversion effiency (~10%) Oxygen production inhibits Fe-hydrogenase Has not yet been overcome after 60 years of research

6 A hydrogen-producing C. reinhardtii culture.

7 Photosystem II Oxidation of Indegenous Cellular Substrate

8 Hydrogenase-related electron transport pathways in green algae.



11 Coordinated photosynthetic and respiratory electron transport and coupled phosphorylation during H2 production.

12 Regulation of Hydrogenase Grown under photo-autotrphopic condions algae neither consume or produce hydrogen Fe hydrogenase gene induced upon incubation of cells under anaerobic conditions in the dark, or S deprivation Results suggest oxygen is a positive suppresser of Fe hydrogenase gene expression at transcriptional level

13 Possible Solutions to Current Limitations Low light conversion efficiency: Truncate chlorophyll antenna size of PS-II using RNAi method Availability of reduced Ferrodoxin for Fe-Hydrogenase: Change Ferrodoxin affinity for (FNR) without affecting Fe-hydrogenase interaction




17 The Future

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