Presentation is loading. Please wait.

Presentation is loading. Please wait.

Identifying Sources of Microbial Pollution Using Traditional Microbial and Molecular Source Tracking Techniques Julie Gaertner University of Hawai`i Hilo.

Published byCitlali Calcutt Modified over 9 years ago

Similar presentations

Presentation on theme: "Identifying Sources of Microbial Pollution Using Traditional Microbial and Molecular Source Tracking Techniques Julie Gaertner University of Hawai`i Hilo."— Presentation transcript:

1 Identifying Sources of Microbial Pollution Using Traditional Microbial and Molecular Source Tracking Techniques Julie Gaertner University of Hawai`i Hilo M.S. Tropical Conservation Biology & Environmental Science

2 Microbial Methods Clostridium perfringens Enterococcus spp. Enumerated with Enterolert Kits by IDEXX Laboratories Inc. Enumerated by Membrane Filtration Method Bisson and Cabelli 1979. Photo Credit: Santuarysimon.org Photo Credit: Oxiod.com

3 Molecular Method Human Specific Genetic Markers Bacteroidales 16S rRNA Bacteroides-Prevotella group Length heterogeneity PCR Terminal restriction fragment length polymorphism (T-RFLP) PCR detection of human-specific Bacteroides genetic marker from sewage (Bower et al. 2005).

4 Water Quality Parameters Total Nitrogen Total Dissolved Nitrogen Nitrate + Nitrite Ammonium Total Phosphorus Phosphate Light Levels Salinity Temperature Specific Conductivity Dissolved Oxygen Turbidity pH Weather & Climate Data

5 High Resolution GIS Mapping CYCLOPS-7 Optical Brightener Fluorometer Probe DataBank Data Logger – GPS Enabled Map Credit: Ambyr-Mokiao-Lee Photo Credit: Turnerdesigns.com

6 Acknowledgements Dr. Tracy Wiegner Dr. Jon Awaya Dr. Stephanie Molloy Dr. Misaki Takabayashi Dr. Mazen Hamad Ambyr Mokiao-Lee Eric Johnson Lucas Mead Narrissa Spies Steven Bradshaw University of Hawai`i Hilo EPSCoR Analytical Hawai`i Department of Health

Download ppt "Identifying Sources of Microbial Pollution Using Traditional Microbial and Molecular Source Tracking Techniques Julie Gaertner University of Hawai`i Hilo."

Similar presentations

West Virginia Department of Agriculture Water Quality Monitoring Program 2009 Photo by Paul Morley.

West Virginia Department of Agriculture Water Quality Monitoring Program 2009 Photo by Paul Morley.
Gina Barrier NW Outreach Coordinator College of Physical and Mathematical Sciences.

Gina Barrier NW Outreach Coordinator College of Physical and Mathematical Sciences.
Erik Johnson TCBES Masters program University of Hawaii at Hilo Surface water metabolism potential of groundwater-fed near shore waters on the leeward.

Erik Johnson TCBES Masters program University of Hawaii at Hilo Surface water metabolism potential of groundwater-fed near shore waters on the leeward.
Corals: ‘canaries’ in coastal communities? Misaki Takabayashi, John Burns, Narrissa Spies, Makani Gregg, Patrick Yasukawa Marine Science Department / Tropical.

Corals: ‘canaries’ in coastal communities? Misaki Takabayashi, John Burns, Narrissa Spies, Makani Gregg, Patrick Yasukawa Marine Science Department / Tropical.
Spatial and Temporal Nutrient Patterns in Coastal Waters of Kaloko-Honokōhau Tracy Wiegner 1, Jason Adolf 1, Jim Beets 1, Rebecca Most 2, Erik Johnson.

Spatial and Temporal Nutrient Patterns in Coastal Waters of Kaloko-Honokōhau Tracy Wiegner 1, Jason Adolf 1, Jim Beets 1, Rebecca Most 2, Erik Johnson.
The MOBI Aerator in the Stockton Deep Water Ship Channel Gary M. Litton Russ Brown Marshall Haueter Stephanie Kong Nitrification in the San Joaquin River.

The MOBI Aerator in the Stockton Deep Water Ship Channel Gary M. Litton Russ Brown Marshall Haueter Stephanie Kong Nitrification in the San Joaquin River.
Water Quality Analysis of the Golden Tract West Wetland Mitigation at New Brittany Kelli R Braightmeyer and Bruce B Smith Department of Biological Science,

Water Quality Analysis of the Golden Tract West Wetland Mitigation at New Brittany Kelli R Braightmeyer and Bruce B Smith Department of Biological Science,
1.4.5 Environmental Factors. 2 Need to know Define and give examples of the following as applied to terrestrial (land) and aquatic (water) environments:

1.4.5 Environmental Factors. 2 Need to know Define and give examples of the following as applied to terrestrial (land) and aquatic (water) environments:
The Environment By: Kyle Olivia Jessica Heather Sarah Tyler.

The Environment By: Kyle Olivia Jessica Heather Sarah Tyler.
West Virginia Non-tidal Monitoring Network: 2010 Update West Virginia Department of Environmental Protection West Virginia Department of Agriculture USGS.

West Virginia Non-tidal Monitoring Network: 2010 Update West Virginia Department of Environmental Protection West Virginia Department of Agriculture USGS.
Lecture 21: Molecular Tools of Genetic Diagnosis Reading Assignment: Chapter 42, pgs. 488-504; Harper’s Biochemistry (25 th edition). Objective: To understand.

Lecture 21: Molecular Tools of Genetic Diagnosis Reading Assignment: Chapter 42, pgs ; Harper’s Biochemistry (25 th edition). Objective: To understand.
Assessing Aquatic Ecosystems & Measurement. Aquatic Ecosystem Assessment The health of an aquatic ecosystem can be determined by examining a variety of.

Assessing Aquatic Ecosystems & Measurement. Aquatic Ecosystem Assessment The health of an aquatic ecosystem can be determined by examining a variety of.
T EACHER W ORKSHOP Dr. Carla M. Koretsky Department of Geosciences Environmental Studies Program Western Michigan University July 18, 2011.

T EACHER W ORKSHOP Dr. Carla M. Koretsky Department of Geosciences Environmental Studies Program Western Michigan University July 18, 2011.
Bioaugmentation and Biostimulation

Bioaugmentation and Biostimulation
Vibrio species are present in The Great Bay, Durham, NH. All three species which are monitored, V. parahaemolyticus, V. vulnificus, and V. cholerae, were.

Vibrio species are present in The Great Bay, Durham, NH. All three species which are monitored, V. parahaemolyticus, V. vulnificus, and V. cholerae, were.
Problem Description: To develop an autonomous network for monitoring aquatic environment Problem Description: To develop an autonomous network for monitoring.

Problem Description: To develop an autonomous network for monitoring aquatic environment Problem Description: To develop an autonomous network for monitoring.
Examination of Cyanobacteria (Blue-Green Algae) Microcystin- LR Toxin in Callander Bay Presented by: Jamie Lavigne Supervised by: Dr. Reehan Mirza Department.

Examination of Cyanobacteria (Blue-Green Algae) Microcystin- LR Toxin in Callander Bay Presented by: Jamie Lavigne Supervised by: Dr. Reehan Mirza Department.
Water Quality Analysis Methods and Applications. Water Quality Analysis Physical Factors including suspended materials (called suspended solids) and dissolved.

Water Quality Analysis Methods and Applications. Water Quality Analysis Physical Factors including suspended materials (called suspended solids) and dissolved.
Flowchart for the Laboratory Sampling Sample Subsample Sub- suplying lab LIMS Analyte A Analyte B Analyte C Analyte D Analyte Y Result A Result B Result.

Flowchart for the Laboratory Sampling Sample Subsample Sub- suplying lab LIMS Analyte A Analyte B Analyte C Analyte D Analyte Y Result A Result B Result.
Using GIS with Real-time Water Quality Assessment to Guide Scientific Inquiry and Learning in a Community College Environmental Studies Program Matthew.

Using GIS with Real-time Water Quality Assessment to Guide Scientific Inquiry and Learning in a Community College Environmental Studies Program Matthew.

Similar presentations

Ads by Google